Values CatLog read from this paper
Kinetic analysis of Escherichia coli 2-C-methyl-D-erythritol-4-phosphate cytidyltransferase, wild type and mutants, reveals roles of active site amino acids
PMID 15379557doi:10.1021/bi0487241Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 47 records (65 values) from this paper: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 0.37 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD), 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 25.9 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD), 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 48.4 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD), 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 131 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD), 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) | Escherichia coli | cytidine 5'-triphosphate | 54.1 | 0.76 | 71 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD), cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant D106E | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 15.5 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant D106E, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant D106E | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 0.54 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant D106E, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant D106E | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 0.9 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant D106E, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant D106E | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 0.06 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant D106E, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant D106E | Escherichia coli | cytidine 5'-triphosphate | 0.93 | 1.4 | 0.66 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant D106E, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant K27S | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 3 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant K27S, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant K27S | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 0.04 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant K27S, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant K27S | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 0.75 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant K27S, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant K27S | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 0.25 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant K27S, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant K27S | Escherichia coli | cytidine 5'-triphosphate | 2.05 | 1.1 | 0.5 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant K27S, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R109A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 131.5 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R109A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R109A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 0.27 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R109A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R109A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 0.4 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R109A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R109A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 0.003 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R109A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R109A | Escherichia coli | cytidine 5'-triphosphate | 0.26 | 3 | 0.1 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R109A, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R109A | Escherichia coli | cytidine 5'-triphosphateapparent kcat | 0.17 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R109A, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 2.98 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 19.6 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 36.8 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 12 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19A | Escherichia coli | cytidine 5'-triphosphate | 27.5 | 2.8 | 10 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19A, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 0.74 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 23.2 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 47.2 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 64 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R19K | Escherichia coli | cytidine 5'-triphosphate | 46.4 | 0.85 | 54.6 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R19K, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 3.28 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 22.6 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 52.7 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20A | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 16 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20A, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20A | Escherichia coli | cytidine 5'-triphosphate | 40.2 | 6.6 | 6 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20A, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 0.31 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 6.7 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 21.4 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20K | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 69 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20K, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant R20K | Escherichia coli | cytidine 5'-triphosphate | 53.6 | 3.7 | 14.5 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant R20K, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant T140V | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | 49 | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant T140V, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant T140V | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphateapparent kcat | 2.7 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant T140V, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant T140V | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | 4 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant T140V, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant T140V | Escherichia coli | 2-C-methyl-D-erythritol 4-phosphate | — | — | 0.08 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant T140V, 2-C-methyl-D-erythritol 4-phosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant T140V | Escherichia coli | cytidine 5'-triphosphate | 1.8 | 2 | 0.9 | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant T140V, cytidine 5'-triphosphate |
| 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD)variant T140V | Escherichia coli | cytidine 5'-triphosphateapparent kcat | 1.21 | — | — | 23 °C | Open record: 2-C-methyl-D-erythritol 4-phosphate cytidyltransferase (YgbP, IspD) variant T140V, cytidine 5'-triphosphate |