Values CatLog read from this paper

Alternative substrates for wild-type and L109A E. coli CTP synthases: kinetic evidence for a constricted ammonia tunnel

PMID 15511226doi:10.1111/j.1432-1033.2004.04360.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (42 values) from this paper: CTP synthase from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 6.3.4.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
CTP synthase	Escherichia coli	ammonia	9.5	2.15	4.43	pH 8, 37 °C	Open record: CTP synthase, ammonia
CTP synthase	Escherichia coli	hydrazine	—	—	0.147	pH 8, 37 °C	Open record: CTP synthase, hydrazine
CTP synthase	Escherichia coli	hydroxylamine	14	82.8	0.169	pH 8, 37 °C	Open record: CTP synthase, hydroxylamine
CTP synthase	Escherichia coli	L-gamma-glutamyl hydrazideCTP formation; continuous spectrophotometric assay at 291 nm	1.41	39.4	0.036	pH 8, 37 °C	Open record: CTP synthase, L-gamma-glutamyl hydrazide
CTP synthase	Escherichia coli	L-gamma-glutamyl hydrazide1 mM ATP; 1 mM UTP; 0.25 mM GTP	—	—	0.034	pH 8, 37 °C	Open record: CTP synthase, L-gamma-glutamyl hydrazide
CTP synthase	Escherichia coli	L-gamma-glutamyl hydroxamateCTP formation; continuous spectrophotometric assay at 291 nm	0.453	0.165	2.77	pH 8, 37 °C	Open record: CTP synthase, L-gamma-glutamyl hydroxamate
CTP synthase	Escherichia coli	L-gamma-glutamyl hydroxamate1 mM ATP; 1 mM UTP; 0.25 mM GTP	0.93	0.324	3.06	pH 8, 37 °C	Open record: CTP synthase, L-gamma-glutamyl hydroxamate
CTP synthase	Escherichia coli	L-glutamineCTP formation; continuous spectrophotometric assay at 291 nm	6.1	0.354	17.8	pH 8, 37 °C	Open record: CTP synthase, L-glutamine
CTP synthase	Escherichia coli	L-glutamine1 mM ATP; 1 mM UTP; 0.25 mM GTP	5.62	0.327	17.2	pH 8, 37 °C	Open record: CTP synthase, L-glutamine
CTP synthasevariant L109A	Escherichia coli	ammonia	10.1	2.17	4.63	pH 8, 37 °C	Open record: CTP synthase variant L109A, ammonia
CTP synthasevariant L109A	Escherichia coli	hydrazine	—	—	0.128	pH 8, 37 °C	Open record: CTP synthase variant L109A, hydrazine
CTP synthasevariant L109A	Escherichia coli	hydroxylamine	14.1	75.3	0.187	pH 8, 37 °C	Open record: CTP synthase variant L109A, hydroxylamine
CTP synthasevariant L109A	Escherichia coli	L-gamma-glutamyl hydroxamateCTP formation; continuous spectrophotometric assay at 291 nm	0.063	0.25	0.26	pH 8, 37 °C	Open record: CTP synthase variant L109A, L-gamma-glutamyl hydroxamate
CTP synthasevariant L109A	Escherichia coli	L-gamma-glutamyl hydroxamate1 mM ATP; 1 mM UTP; 0.25 mM GTP	0.31	0.26	1.26	pH 8, 37 °C	Open record: CTP synthase variant L109A, L-gamma-glutamyl hydroxamate
CTP synthasevariant L109A	Escherichia coli	L-glutamineCTP formation; continuous spectrophotometric assay at 291 nm	1.86	0.497	3.85	pH 8, 37 °C	Open record: CTP synthase variant L109A, L-glutamine
CTP synthasevariant L109A	Escherichia coli	L-glutamine1 mM ATP; 1 mM UTP; 0.25 mM GTP	5.06	0.55	9.22	pH 8, 37 °C	Open record: CTP synthase variant L109A, L-glutamine