EC 6.3.4.2: CTP synthase (glutamine hydrolysing)

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CatLog read 86 records for EC 6.3.4.2 from 4 papers, for 2 organisms. Names in these papers: CTP synthase, CTP synthase (EcCTPS), CTP synthase (CTPS).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 81 records for EC 6.3.4.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 6.3.4.2 in Browse.

Papers

Papers with records for EC 6.3.4.2
Paper	Organisms	Records
"Pinching" the ammonia tunnel of CTP synthase unveils coordinated catalytic and allosteric-dependent control of ammonia passage	Escherichia coli	31
Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity	Lactococcus lactis	24
Alternative substrates for wild-type and L109A E. coli CTP synthases: kinetic evidence for a constricted ammonia tunnel	Escherichia coli	16
The role of lysine residues 297 and 306 in nucleoside triphosphate regulation of E. coli CTP synthase: inactivation by 2',3'-dialdehyde ATP and mutational analyses	Escherichia coli	15

Records

Records for EC 6.3.4.2 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
CTP synthase	Escherichia coli	ammonia	9.5	2.15	4.43	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, ammonia
CTP synthase	Escherichia coli	hydrazine	—	—	0.147	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, hydrazine
CTP synthase	Escherichia coli	hydroxylamine	14	82.8	0.169	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, hydroxylamine
CTP synthase	Escherichia coli	L-gamma-glutamyl hydrazideCTP formation; continuous spectrophotometric assay at 291 nm	1.41	39.4	0.036	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, L-gamma-glutamyl hydrazide
CTP synthase	Escherichia coli	L-gamma-glutamyl hydrazide1 mM ATP; 1 mM UTP; 0.25 mM GTP	—	—	0.034	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, L-gamma-glutamyl hydrazide
CTP synthase	Escherichia coli	L-gamma-glutamyl hydroxamateCTP formation; continuous spectrophotometric assay at 291 nm	0.453	0.165	2.77	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, L-gamma-glutamyl hydroxamate
CTP synthase	Escherichia coli	L-gamma-glutamyl hydroxamate1 mM ATP; 1 mM UTP; 0.25 mM GTP	0.93	0.324	3.06	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, L-gamma-glutamyl hydroxamate
CTP synthase	Escherichia coli	L-glutamineCTP formation; continuous spectrophotometric assay at 291 nm	6.1	0.354	17.8	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, L-glutamine
CTP synthase	Escherichia coli	L-glutamine1 mM ATP; 1 mM UTP; 0.25 mM GTP	5.62	0.327	17.2	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase, L-glutamine
CTP synthasevariant L109A	Escherichia coli	ammonia	10.1	2.17	4.63	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, ammonia
CTP synthasevariant L109A	Escherichia coli	hydrazine	—	—	0.128	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, hydrazine
CTP synthasevariant L109A	Escherichia coli	hydroxylamine	14.1	75.3	0.187	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, hydroxylamine
CTP synthasevariant L109A	Escherichia coli	L-gamma-glutamyl hydroxamateCTP formation; continuous spectrophotometric assay at 291 nm	0.063	0.25	0.26	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, L-gamma-glutamyl hydroxamate
CTP synthasevariant L109A	Escherichia coli	L-gamma-glutamyl hydroxamate1 mM ATP; 1 mM UTP; 0.25 mM GTP	0.31	0.26	1.26	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, L-gamma-glutamyl hydroxamate
CTP synthasevariant L109A	Escherichia coli	L-glutamineCTP formation; continuous spectrophotometric assay at 291 nm	1.86	0.497	3.85	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, L-glutamine
CTP synthasevariant L109A	Escherichia coli	L-glutamine1 mM ATP; 1 mM UTP; 0.25 mM GTP	5.06	0.55	9.22	pH 8, 37 °C	PMID 15511226	Open record: CTP synthase variant L109A, L-glutamine
CTP synthase	Lactococcus lactis	ammonium chloride	6.3	54	0.12 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase, ammonium chloride
CTP synthase	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.134	0.94	0.14 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase, L-glutamine
CTP synthase	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.334	0.85	0.39 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase, L-glutamine
CTP synthase	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.11	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase, L-glutamine
CTP synthasevariant E362Q	Lactococcus lactis	ammonium chloride	10.1	78	0.13 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant E362Q, ammonium chloride
CTP synthasevariant E362Q	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.135	0.87	0.16 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant E362Q, L-glutamine
CTP synthasevariant E362Q	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.389	0.87	0.45 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant E362Q, L-glutamine
CTP synthasevariant E362Q	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.1	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant E362Q, L-glutamine
CTP synthasevariant G360A	Lactococcus lactis	ammonium chloride	7.5	56	0.13 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360A, ammonium chloride
CTP synthasevariant G360A	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.125	0.96	0.13 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360A, L-glutamine
CTP synthasevariant G360A	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.608	1.05	0.579 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360A, L-glutamine
CTP synthasevariant G360A	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.2	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360A, L-glutamine
CTP synthasevariant G360P	Lactococcus lactis	ammonium chloride	1.8	92	0.020 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360P, ammonium chloride
CTP synthasevariant G360P	Lactococcus lactis	L-glutaminekcat from a Hill fit, n = 0.68 ± 0.04	0.21	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360P, L-glutamine
CTP synthasevariant G360P	Lactococcus lactis	L-glutaminekcat from a Hill fit, n = 0.70 ± 0.03	0.22	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360P, L-glutamine
CTP synthasevariant G360P	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.01	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant G360P, L-glutamine
CTP synthasevariant R359M	Lactococcus lactis	ammonium chloride	6.9	57	0.12 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359M, ammonium chloride
CTP synthasevariant R359M	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.15	1.29	0.12 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359M, L-glutamine
CTP synthasevariant R359M	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.148	1.01	0.147 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359M, L-glutamine
CTP synthasevariant R359M	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.024	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359M, L-glutamine
CTP synthasevariant R359P	Lactococcus lactis	ammonium chloride	5.1	92	0.055 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359P, ammonium chloride
CTP synthasevariant R359P	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.072	0.74	0.097 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359P, L-glutamine
CTP synthasevariant R359P	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.0785	0.95	0.083 calc.	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359P, L-glutamine
CTP synthasevariant R359P	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.012	—	—	pH 8, 30 °C	PMID 15670165	Open record: CTP synthase variant R359P, L-glutamine
CTP synthase (CTPS)	Escherichia coli	adenosine 5'-triphosphate	12.8	—	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS), adenosine 5'-triphosphate
CTP synthase (CTPS)	Escherichia coli	ammonia	11.3	2.79	4.07	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS), ammonia
CTP synthase (CTPS)	Escherichia coli	L-glutamine[ATP] = 1 mM; [UTP] = 1 mM	—	0.345	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS), L-glutamine
CTP synthase (CTPS)	Escherichia coli	L-glutamine[ATP] = 3 mM; [UTP] = 2 mM (saturating)	1.5	0.206	7.3	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS), L-glutamine
CTP synthase (CTPS)	Escherichia coli	uridine 5'-triphosphate	13.7	—	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS), uridine 5'-triphosphate
CTP synthase (CTPS)variant K297A	Escherichia coli	adenosine 5'-triphosphate	10.8	—	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K297A, adenosine 5'-triphosphate
CTP synthase (CTPS)variant K297A	Escherichia coli	ammonia	8.59	1.54	5.6	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K297A, ammonia
CTP synthase (CTPS)variant K297A	Escherichia coli	L-glutamine	0.077	—	—		PMID 16427816	Open record: CTP synthase (CTPS) variant K297A, L-glutamine
CTP synthase (CTPS)variant K297A	Escherichia coli	uridine 5'-triphosphate	14	—	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K297A, uridine 5'-triphosphate
CTP synthase (CTPS)variant K306A	Escherichia coli	adenosine 5'-triphosphate	6.26	—	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K306A, adenosine 5'-triphosphate
CTP synthase (CTPS)variant K306A	Escherichia coli	ammonia[ATP] = 1 mM; [UTP] = 1 mM	2.19	0.627	3.5	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K306A, ammonia
CTP synthase (CTPS)variant K306A	Escherichia coli	ammonia[ATP] = 3 mM; [UTP] = 2 mM (saturating)	7.59	1.47	5.19	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K306A, ammonia
CTP synthase (CTPS)variant K306A	Escherichia coli	L-glutamine[ATP] = 1 mM; [UTP] = 1 mM	1.28	0.196	6.55	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K306A, L-glutamine
CTP synthase (CTPS)variant K306A	Escherichia coli	L-glutamine[ATP] = 3 mM; [UTP] = 2 mM (saturating)	1.35	0.424	3.27	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K306A, L-glutamine
CTP synthase (CTPS)variant K306A	Escherichia coli	uridine 5'-triphosphate	6.9	—	—	pH 8, 37 °C	PMID 16427816	Open record: CTP synthase (CTPS) variant K306A, uridine 5'-triphosphate
CTP synthase (EcCTPS)	Escherichia coli	ammonia	7.7	1.4	5.3	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS), ammonia
CTP synthase (EcCTPS)	Escherichia coli	hydroxylamine	13.4	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS), hydroxylamine
CTP synthase (EcCTPS)variant V60A	Escherichia coli	adenosine 5'-triphosphate	5.6	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60A, adenosine 5'-triphosphate
CTP synthase (EcCTPS)variant V60A	Escherichia coli	ammoniaionic strength 0.15 M with KCl	3.3	0.7	5.1	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60A, ammonia
CTP synthase (EcCTPS)variant V60A	Escherichia coli	ammoniaionic strength 0.15 M with NaOAc	3.12	0.61	5.1	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60A, ammonia
CTP synthase (EcCTPS)variant V60A	Escherichia coli	L-glutamine	5.2	0.37	14.2	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60A, L-glutamine
CTP synthase (EcCTPS)variant V60A	Escherichia coli	uridine 5'-triphosphate	5.69	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60A, uridine 5'-triphosphate
CTP synthase (EcCTPS)variant V60C	Escherichia coli	adenosine 5'-triphosphate	5.1	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60C, adenosine 5'-triphosphate
CTP synthase (EcCTPS)variant V60C	Escherichia coli	ammoniaionic strength 0.15 M with KCl	5.7	3.3	1.8	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60C, ammonia
CTP synthase (EcCTPS)variant V60C	Escherichia coli	ammoniaionic strength 0.15 M with NaOAc	7.5	4.2	1.8	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60C, ammonia
CTP synthase (EcCTPS)variant V60C	Escherichia coli	L-glutamine	5.14	0.24	21.6	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60C, L-glutamine
CTP synthase (EcCTPS)variant V60C	Escherichia coli	uridine 5'-triphosphate	5.1	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60C, uridine 5'-triphosphate
CTP synthase (EcCTPS)variant V60D	Escherichia coli	adenosine 5'-triphosphate	1.5	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60D, adenosine 5'-triphosphate
CTP synthase (EcCTPS)variant V60D	Escherichia coli	ammoniaionic strength 0.15 M with KCl	0.99	3.6	0.27	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60D, ammonia
CTP synthase (EcCTPS)variant V60D	Escherichia coli	ammoniaionic strength 0.15 M with NaOAc	1.1	1.6	0.7	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60D, ammonia
CTP synthase (EcCTPS)variant V60D	Escherichia coli	L-glutamine	1.6	0.5	2.9	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60D, L-glutamine
CTP synthase (EcCTPS)variant V60D	Escherichia coli	uridine 5'-triphosphate	1.7	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60D, uridine 5'-triphosphate
CTP synthase (EcCTPS)variant V60F	Escherichia coli	adenosine 5'-triphosphate	0.99	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, adenosine 5'-triphosphate
CTP synthase (EcCTPS)variant V60F	Escherichia coli	adenosine 5'-triphosphateNH3 source 150 mM NH4OAc (Table 2 footnote g); 1.0 mM GTP	1.68	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, adenosine 5'-triphosphate
CTP synthase (EcCTPS)variant V60F	Escherichia coli	ammoniaionic strength 0.15 M with KCl	1.2	1.6	0.73	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, ammonia
CTP synthase (EcCTPS)variant V60F	Escherichia coli	ammoniaionic strength 0.15 M with NaOAc	2.2	3.1	0.72	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, ammonia
CTP synthase (EcCTPS)variant V60F	Escherichia coli	ammoniaionic strength 0.15 M with NaOAc	0.16	0.94	0.17	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, ammonia
CTP synthase (EcCTPS)variant V60F	Escherichia coli	hydroxylamine	5	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, hydroxylamine
CTP synthase (EcCTPS)variant V60F	Escherichia coli	L-glutamine	0.82	5.9	0.14	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, L-glutamine
CTP synthase (EcCTPS)variant V60F	Escherichia coli	uridine 5'-triphosphate	1.05	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, uridine 5'-triphosphate
CTP synthase (EcCTPS)variant V60F	Escherichia coli	uridine 5'-triphosphateNH3 source 150 mM NH4OAc (Table 2 footnote g); 1.0 mM GTP	1.86	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60F, uridine 5'-triphosphate
CTP synthase (EcCTPS)variant V60W	Escherichia coli	adenosine 5'-triphosphate	1.46	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60W, adenosine 5'-triphosphate
CTP synthase (EcCTPS)variant V60W	Escherichia coli	ammoniaionic strength 0.15 M with KCl	0.95	4.1	0.25	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60W, ammonia
CTP synthase (EcCTPS)variant V60W	Escherichia coli	ammoniaionic strength 0.15 M with NaOAc	1.6	1.5	1.1	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60W, ammonia
CTP synthase (EcCTPS)variant V60W	Escherichia coli	L-glutamine	0.08	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60W, L-glutamine
CTP synthase (EcCTPS)variant V60W	Escherichia coli	uridine 5'-triphosphate	1.4	—	—	pH 8, 37 °C	PMID 30251661	Open record: CTP synthase (EcCTPS) variant V60W, uridine 5'-triphosphate