Values CatLog read from this paper

Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity

PMID 15670165doi:10.1111/j.1742-4658.2004.04525.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (40 values) from this paper: CTP synthase from Lactococcus lactis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 16 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 6.3.4.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
CTP synthase	Lactococcus lactis	ammonium chloride	6.3	54	0.12 calc.	pH 8, 30 °C	Open record: CTP synthase, ammonium chloride
CTP synthase	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.134	0.94	0.14 calc.	pH 8, 30 °C	Open record: CTP synthase, L-glutamine
CTP synthase	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.334	0.85	0.39 calc.	pH 8, 30 °C	Open record: CTP synthase, L-glutamine
CTP synthase	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.11	—	—	pH 8, 30 °C	Open record: CTP synthase, L-glutamine
CTP synthasevariant E362Q	Lactococcus lactis	ammonium chloride	10.1	78	0.13 calc.	pH 8, 30 °C	Open record: CTP synthase variant E362Q, ammonium chloride
CTP synthasevariant E362Q	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.135	0.87	0.16 calc.	pH 8, 30 °C	Open record: CTP synthase variant E362Q, L-glutamine
CTP synthasevariant E362Q	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.389	0.87	0.45 calc.	pH 8, 30 °C	Open record: CTP synthase variant E362Q, L-glutamine
CTP synthasevariant E362Q	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.1	—	—	pH 8, 30 °C	Open record: CTP synthase variant E362Q, L-glutamine
CTP synthasevariant G360A	Lactococcus lactis	ammonium chloride	7.5	56	0.13 calc.	pH 8, 30 °C	Open record: CTP synthase variant G360A, ammonium chloride
CTP synthasevariant G360A	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.125	0.96	0.13 calc.	pH 8, 30 °C	Open record: CTP synthase variant G360A, L-glutamine
CTP synthasevariant G360A	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.608	1.05	0.579 calc.	pH 8, 30 °C	Open record: CTP synthase variant G360A, L-glutamine
CTP synthasevariant G360A	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.2	—	—	pH 8, 30 °C	Open record: CTP synthase variant G360A, L-glutamine
CTP synthasevariant G360P	Lactococcus lactis	ammonium chloride	1.8	92	0.020 calc.	pH 8, 30 °C	Open record: CTP synthase variant G360P, ammonium chloride
CTP synthasevariant G360P	Lactococcus lactis	L-glutaminekcat from a Hill fit, n = 0.68 ± 0.04	0.21	—	—	pH 8, 30 °C	Open record: CTP synthase variant G360P, L-glutamine
CTP synthasevariant G360P	Lactococcus lactis	L-glutaminekcat from a Hill fit, n = 0.70 ± 0.03	0.22	—	—	pH 8, 30 °C	Open record: CTP synthase variant G360P, L-glutamine
CTP synthasevariant G360P	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.01	—	—	pH 8, 30 °C	Open record: CTP synthase variant G360P, L-glutamine
CTP synthasevariant R359M	Lactococcus lactis	ammonium chloride	6.9	57	0.12 calc.	pH 8, 30 °C	Open record: CTP synthase variant R359M, ammonium chloride
CTP synthasevariant R359M	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.15	1.29	0.12 calc.	pH 8, 30 °C	Open record: CTP synthase variant R359M, L-glutamine
CTP synthasevariant R359M	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.148	1.01	0.147 calc.	pH 8, 30 °C	Open record: CTP synthase variant R359M, L-glutamine
CTP synthasevariant R359M	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.024	—	—	pH 8, 30 °C	Open record: CTP synthase variant R359M, L-glutamine
CTP synthasevariant R359P	Lactococcus lactis	ammonium chloride	5.1	92	0.055 calc.	pH 8, 30 °C	Open record: CTP synthase variant R359P, ammonium chloride
CTP synthasevariant R359P	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.072	0.74	0.097 calc.	pH 8, 30 °C	Open record: CTP synthase variant R359P, L-glutamine
CTP synthasevariant R359P	Lactococcus lactis	L-glutamineapparent Km; Table 2	0.0785	0.95	0.083 calc.	pH 8, 30 °C	Open record: CTP synthase variant R359P, L-glutamine
CTP synthasevariant R359P	Lactococcus lactis	L-glutamine10 mM glutamine; 1 mM ATP; 1 mM UTP; cosubstrate ATP, UTP	0.012	—	—	pH 8, 30 °C	Open record: CTP synthase variant R359P, L-glutamine