Values CatLog read from this paper
"Pinching" the ammonia tunnel of CTP synthase unveils coordinated catalytic and allosteric-dependent control of ammonia passage
PMID 30251661doi:10.1016/j.bbagen.2018.08.008Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 31 records (63 values) from this paper: CTP synthase (EcCTPS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| CTP synthase (EcCTPS) | Escherichia coli | ammonia | 7.7 | 1.4 | 5.3 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS), ammonia |
| CTP synthase (EcCTPS) | Escherichia coli | hydroxylamine | 13.4 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS), hydroxylamine |
| CTP synthase (EcCTPS)variant V60A | Escherichia coli | adenosine 5'-triphosphate | 5.6 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60A, adenosine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60A | Escherichia coli | ammoniaionic strength 0.15 M with KCl | 3.3 | 0.7 | 5.1 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60A, ammonia |
| CTP synthase (EcCTPS)variant V60A | Escherichia coli | ammoniaionic strength 0.15 M with NaOAc | 3.12 | 0.61 | 5.1 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60A, ammonia |
| CTP synthase (EcCTPS)variant V60A | Escherichia coli | L-glutamine | 5.2 | 0.37 | 14.2 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60A, L-glutamine |
| CTP synthase (EcCTPS)variant V60A | Escherichia coli | uridine 5'-triphosphate | 5.69 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60A, uridine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60C | Escherichia coli | adenosine 5'-triphosphate | 5.1 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60C, adenosine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60C | Escherichia coli | ammoniaionic strength 0.15 M with KCl | 5.7 | 3.3 | 1.8 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60C, ammonia |
| CTP synthase (EcCTPS)variant V60C | Escherichia coli | ammoniaionic strength 0.15 M with NaOAc | 7.5 | 4.2 | 1.8 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60C, ammonia |
| CTP synthase (EcCTPS)variant V60C | Escherichia coli | L-glutamine | 5.14 | 0.24 | 21.6 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60C, L-glutamine |
| CTP synthase (EcCTPS)variant V60C | Escherichia coli | uridine 5'-triphosphate | 5.1 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60C, uridine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60D | Escherichia coli | adenosine 5'-triphosphate | 1.5 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60D, adenosine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60D | Escherichia coli | ammoniaionic strength 0.15 M with KCl | 0.99 | 3.6 | 0.27 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60D, ammonia |
| CTP synthase (EcCTPS)variant V60D | Escherichia coli | ammoniaionic strength 0.15 M with NaOAc | 1.1 | 1.6 | 0.7 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60D, ammonia |
| CTP synthase (EcCTPS)variant V60D | Escherichia coli | L-glutamine | 1.6 | 0.5 | 2.9 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60D, L-glutamine |
| CTP synthase (EcCTPS)variant V60D | Escherichia coli | uridine 5'-triphosphate | 1.7 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60D, uridine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | adenosine 5'-triphosphate | 0.99 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, adenosine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | adenosine 5'-triphosphateNH3 source 150 mM NH4OAc (Table 2 footnote g); 1.0 mM GTP | 1.68 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, adenosine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | ammoniaionic strength 0.15 M with KCl | 1.2 | 1.6 | 0.73 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, ammonia |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | ammoniaionic strength 0.15 M with NaOAc | 2.2 | 3.1 | 0.72 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, ammonia |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | ammoniaionic strength 0.15 M with NaOAc | 0.16 | 0.94 | 0.17 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, ammonia |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | hydroxylamine | 5 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, hydroxylamine |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | L-glutamine | 0.82 | 5.9 | 0.14 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, L-glutamine |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | uridine 5'-triphosphate | 1.05 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, uridine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60F | Escherichia coli | uridine 5'-triphosphateNH3 source 150 mM NH4OAc (Table 2 footnote g); 1.0 mM GTP | 1.86 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60F, uridine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60W | Escherichia coli | adenosine 5'-triphosphate | 1.46 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60W, adenosine 5'-triphosphate |
| CTP synthase (EcCTPS)variant V60W | Escherichia coli | ammoniaionic strength 0.15 M with KCl | 0.95 | 4.1 | 0.25 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60W, ammonia |
| CTP synthase (EcCTPS)variant V60W | Escherichia coli | ammoniaionic strength 0.15 M with NaOAc | 1.6 | 1.5 | 1.1 | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60W, ammonia |
| CTP synthase (EcCTPS)variant V60W | Escherichia coli | L-glutamine | 0.08 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60W, L-glutamine |
| CTP synthase (EcCTPS)variant V60W | Escherichia coli | uridine 5'-triphosphate | 1.4 | — | — | pH 8, 37 °C | Open record: CTP synthase (EcCTPS) variant V60W, uridine 5'-triphosphate |