Values CatLog read from this paper
Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes
PMID 15522862doi:10.1074/jbc.m411870200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (34 values) from this paper: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) from Nicotiana tabacum.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.582 | 0.00174 | 334.4 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | 3alpha-hydroxy-5-epiaristolochene | 0.2 | 0.00638 | 31.3 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 3alpha-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | 5-epiaristolochene | 0.493 | 0.01918 | 25.7 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | NADPH | — | 0.061 | — | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), NADPH |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant I486A | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.132 | 0.02124 | 6.2 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant I486A, 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant I486A | Nicotiana tabacum | 5-epiaristolochene | 0.293 | 0.0688 | 4.3 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant I486A, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368A | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.581 | 0.0062 | 93.7 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368A, 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368A | Nicotiana tabacum | 5-epiaristolochene | 0.154 | 0.02267 | 6.8 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368A, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368C | Nicotiana tabacum | 5-epiaristolochene | 0.278 | 0.03863 | 7.2 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368C, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368T | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.482 | 0.00997 | 48.3 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368T, 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368T | Nicotiana tabacum | 5-epiaristolochene | 0.037 | 0.01518 | 2.4 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368T, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368V | Nicotiana tabacum | 5-epiaristolochene | 0.553 | 0.01672 | 33.1 | pH 7.5, 30 °C | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368V, 5-epiaristolochene |