EC 1.14.14.149: 5-epiaristolochene 1,3-dihydroxylase
OxidoreductasesOpen in Browse
CatLog read 16 records for EC 1.14.14.149 from 2 papers, for 1 organism. Names in these papers: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 5-epi-aristolochene hydroxylase (EAH, CYP71D20).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes | Nicotiana tabacum | 12 |
| Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates | Nicotiana tabacum | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| 5-epi-aristolochene hydroxylase (EAH, CYP71D20) | Nicotiana tabacum | premnaspirodiene | 0.4 | 0.0092 | 40 | pH 7.5, 30 °C | PMID 17715131 | Open record: 5-epi-aristolochene hydroxylase (EAH, CYP71D20), premnaspirodiene |
| 5-epi-aristolochene hydroxylase (EAH, CYP71D20)variant S368V | Nicotiana tabacum | premnaspirodiene | 0.6 | 0.0114 | 50 | pH 7.5, 30 °C | PMID 17715131 | Open record: 5-epi-aristolochene hydroxylase (EAH, CYP71D20) variant S368V, premnaspirodiene |
| 5-epi-aristolochene hydroxylase (EAH, CYP71D20)variant S368V/I484V | Nicotiana tabacum | premnaspirodiene | 0.4 | 0.0076 | 50 | pH 7.5, 30 °C | PMID 17715131 | Open record: 5-epi-aristolochene hydroxylase (EAH, CYP71D20) variant S368V/I484V, premnaspirodiene |
| 5-epi-aristolochene hydroxylase (EAH, CYP71D20)variant S368V/S482V | Nicotiana tabacum | premnaspirodiene | 0.6 | 0.0065 | 90 | pH 7.5, 30 °C | PMID 17715131 | Open record: 5-epi-aristolochene hydroxylase (EAH, CYP71D20) variant S368V/S482V, premnaspirodiene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.582 | 0.00174 | 334.4 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | 3alpha-hydroxy-5-epiaristolochene | 0.2 | 0.00638 | 31.3 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 3alpha-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | 5-epiaristolochene | 0.493 | 0.01918 | 25.7 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20) | Nicotiana tabacum | NADPH | — | 0.061 | — | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20), NADPH |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant I486A | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.132 | 0.02124 | 6.2 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant I486A, 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant I486A | Nicotiana tabacum | 5-epiaristolochene | 0.293 | 0.0688 | 4.3 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant I486A, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368A | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.581 | 0.0062 | 93.7 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368A, 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368A | Nicotiana tabacum | 5-epiaristolochene | 0.154 | 0.02267 | 6.8 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368A, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368C | Nicotiana tabacum | 5-epiaristolochene | 0.278 | 0.03863 | 7.2 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368C, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368T | Nicotiana tabacum | 1beta-hydroxy-5-epiaristolochene | 0.482 | 0.00997 | 48.3 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368T, 1beta-hydroxy-5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368T | Nicotiana tabacum | 5-epiaristolochene | 0.037 | 0.01518 | 2.4 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368T, 5-epiaristolochene |
| 5-epiaristolochene dihydroxylase (EAH, CYP71D20)variant S368V | Nicotiana tabacum | 5-epiaristolochene | 0.553 | 0.01672 | 33.1 | pH 7.5, 30 °C | PMID 15522862 | Open record: 5-epiaristolochene dihydroxylase (EAH, CYP71D20) variant S368V, 5-epiaristolochene |