CatLogenzyme kinetics catalog

Values CatLog read from this paper

Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates

PMID 17715131doi:10.1074/jbc.m703378200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 21 records (62 values) from this paper: premnaspirodiene oxygenase (HPO, CYP71D55), 5-epi-aristolochene hydroxylase (EAH, CYP71D20) from Hyoscyamus muticus, Nicotiana tabacum.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 1.14.14.149, EC 1.14.14.151

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
5-epi-aristolochene hydroxylase (EAH, CYP71D20)Nicotiana tabacumpremnaspirodiene0.40.009240pH 7.5, 30 °COpen
5-epi-aristolochene hydroxylase (EAH, CYP71D20)variant S368VNicotiana tabacumpremnaspirodiene0.60.011450pH 7.5, 30 °COpen
5-epi-aristolochene hydroxylase (EAH, CYP71D20)variant S368V/I484VNicotiana tabacumpremnaspirodiene0.40.007650pH 7.5, 30 °COpen
5-epi-aristolochene hydroxylase (EAH, CYP71D20)variant S368V/S482VNicotiana tabacumpremnaspirodiene0.60.006590pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticus(-)-alpha-cedrene0.10.02653.8 calc.pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticus(2S,4R,5S,7R)-spirovetiva-1(10),11(12)-dien-2-ol0.10.001280pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticus4-epi-eremophilene0.80.0078100pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticus5-epi-aristolochene0.20.003360pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticuspremnaspirodieneproduct: solavetivol2.10.014150pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticuspremnaspirodieneproduct: solavetivone0.10.001760pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticusValenceneproduct: α-nootkatol (2-epi-nootkatol)0.10.011510pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)Hyoscyamus muticusValenceneproduct: β-nootkatol (nootkatol)1.90.0074260pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V480I/A484IHyoscyamus muticus5-epi-aristolochene0.70.0025280pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V480I/A484IHyoscyamus muticuspremnaspirodiene12.70.00841.51 × 10³pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V480I/A484IHyoscyamus muticusValencene5.70.0192300pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V482IHyoscyamus muticus5-epi-aristolochene1.40.017980pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V482IHyoscyamus muticuspremnaspirodiene3.80.0062610pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V482IHyoscyamus muticusValencene5.10.0069740pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V482I/A484IHyoscyamus muticus5-epi-aristolochene2.80.0043650pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V482I/A484IHyoscyamus muticuspremnaspirodiene20.70.0131.59 × 10³pH 7.5, 30 °COpen
premnaspirodiene oxygenase (HPO, CYP71D55)variant V482I/A484IHyoscyamus muticusValencene15.90.01211.31 × 10³pH 7.5, 30 °COpen