Values CatLog read from this paper
Nucleotide specificity of HIV-1 reverse transcriptases with amino acid substitutions affecting Ala-114
PMID 15548134doi:10.1042/bj20041056Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 43 records (125 values) from this paper: HIV-1 RT (reverse transcriptase) from HIV-1.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| HIV-1 RT (reverse transcriptase) | HIV-1 | 3'-azido-3'-deoxythymidine triphosphate | 0.0605 | 0.00242 | 25.15 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), 3'-azido-3'-deoxythymidine triphosphate |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dATPtemplate-primer D2cg/20PG5cg (38/20-mer DNA-DNA) | 0.0542 | 4.7 × 10⁻⁵ | 1160 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dATP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dATPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 3 × 10⁻⁴ | — | — | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dATP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dCTPtemplate-primer D38G/25PGG (DNA-DNA); D38G/25PGG (DNA-DNA) | 0.0498 | 1 × 10⁻⁴ | 495.7 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dCTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dCTPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 0.0273 | 1.693 | 0.0162 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dCTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | ddATP | 0.0388 | 6.36 × 10⁻⁴ | 61.2 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), ddATP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | ddCTP | 0.0222 | 9.6 × 10⁻⁴ | 23 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), ddCTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | ddTTP | 0.082 | 0.00856 | 9.58 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), ddTTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dGTP | 0.0273 | 0.537 | 0.052 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dGTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dTTPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 0.059 | 3.2 × 10⁻⁴ | 184.7 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dTTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dTTPtemplate-primer D2-47/PG5-25C | 0.0217 | 0.0413 | 0.53 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dTTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dTTPtemplate-primer D2-47/PG5-25A | 0.0025 | 0.266 | 0.0095 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dTTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | dTTPtemplate-primer D2-47/PG5-25G | 0.0032 | 0.513 | 0.0062 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), dTTP |
| HIV-1 RT (reverse transcriptase) | HIV-1 | rATP | 0.0172 | 0.62 | 0.028 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase), rATP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | 3'-azido-3'-deoxythymidine triphosphate | 0.0187 | 0.0091 | 2.05 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, 3'-azido-3'-deoxythymidine triphosphate |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dATP | 0.0898 | 0.00485 | 18.5 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dATP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dCTPtemplate-primer D38G/25PGG (DNA-DNA); D38G/25PGG (DNA-DNA) | 0.0408 | 0.00134 | 30.5 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dCTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dCTPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 0.00128 | 3.359 | 3.8 × 10⁻⁴ | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dCTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | ddATP | 0.0267 | 0.129 | 0.2 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, ddATP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | ddCTP | 0.0067 | 0.0265 | 0.248 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, ddCTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | ddTTP | 0.0188 | 0.385 | 0.048 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, ddTTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dGTP | 0.003 | 0.91 | 0.0033 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dGTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dTTPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 0.0233 | 0.00407 | 5.7 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dTTPtemplate-primer D2-47/PG5-25C | 0.013 | 0.858 | 0.0152 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dTTPtemplate-primer D2-47/PG5-25A | 5.3 × 10⁻⁴ | 0.824 | 6.5 × 10⁻⁴ | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | dTTPtemplate-primer D2-47/PG5-25G | 3.7 × 10⁻⁴ | 0.23 | 0.0016 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114G | HIV-1 | rATP | 7.5 × 10⁻⁴ | 0.577 | 0.00132 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114G, rATP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | 3'-azido-3'-deoxythymidine triphosphate | 0.0865 | 0.0115 | 7.52 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, 3'-azido-3'-deoxythymidine triphosphate |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dATP | 0.1718 | 0.00122 | 140.8 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dATP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dCTPtemplate-primer D38G/25PGG (DNA-DNA); D38G/25PGG (DNA-DNA) | 0.0917 | 5.75 × 10⁻⁴ | 159.3 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dCTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dCTPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 0.0083 | 1.408 | 0.006 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dCTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | ddATP | 0.037 | 0.00548 | 6.8 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, ddATP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | ddCTP | 0.0132 | 7.97 × 10⁻⁴ | 16.5 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, ddCTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | ddTTP | 0.0138 | 0.00456 | 3.03 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, ddTTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dGTP | 0.0028 | 0.121 | 0.023 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dGTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dTTPtemplate-primer D2-47/PG5-25 (47/25-mer DNA-DNA) | 0.0772 | 0.00202 | 38.2 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dTTPtemplate-primer D2-47/PG5-25C | 0.0273 | 0.28 | 0.098 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dTTPtemplate-primer D2-47/PG5-25A | 0.00163 | 0.319 | 0.0052 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | dTTPtemplate-primer D2-47/PG5-25G | 0.00187 | 0.415 | 0.0045 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114S | HIV-1 | rATP | 0.0077 | 0.83 | 0.0092 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114S, rATP |
| HIV-1 RT (reverse transcriptase)variant A114T | HIV-1 | dTTP | 0.088 | — | — | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114T, dTTP |
| HIV-1 RT (reverse transcriptase)variant A114V | HIV-1 | ddTTP | 0.0342 | 0.412 | 0.083 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114V, ddTTP |
| HIV-1 RT (reverse transcriptase)variant A114V | HIV-1 | dTTP | 0.0788 | 0.071 | 1.12 | pH 7, 37 °C | Open record: HIV-1 RT (reverse transcriptase) variant A114V, dTTP |