Values CatLog read from this paper
Mechanistic roles of Ser-114, Tyr-155, and Lys-159 in 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni
PMID 15572373doi:10.1074/jbc.m411751200Open in Browse
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CatLog read 11 records (26 values) from this paper: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) from Comamonas testosteroni.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) | Comamonas testosteroni | androsterone | 318 | 0.0055 | 5.8 × 10⁴ | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR), androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) | Comamonas testosteroni | androsterone | — | — | 4.3 × 10⁴ | 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR), androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant K159A | Comamonas testosteroni | androsterone | 18.9 | 0.065 | 290 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant K159A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant K159A | Comamonas testosteroni | androsterone | 16 | — | 240 | 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant K159A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant S114A | Comamonas testosteroni | androsterone | 0.12 | 0.007 | 17 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant S114A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant S114A | Comamonas testosteroni | androsterone | — | — | 7.3 | 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant S114A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant S114A/Y155F | Comamonas testosteroni | androsterone | 0.0028 | 4.4 × 10⁻⁴ | 6.2 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant S114A/Y155F, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant Y155F | Comamonas testosteroni | androsterone | 1.5 | 0.074 | 20 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant Y155F, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant Y155F | Comamonas testosteroni | androsterone | 1.2 | — | 17 | 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant Y155F, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant Y155F/K159A | Comamonas testosteroni | androsterone | 0.003 | 0.1 | 0.03 | pH 10.5, 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant Y155F/K159A, androsterone |
| 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR)variant Y155F/K159A | Comamonas testosteroni | androsterone | 0.0046 | — | 0.071 | 25 °C | Open record: 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase (3alpha-HSD/CR) variant Y155F/K159A, androsterone |