Values CatLog read from this paper
The carboligation reaction of acetohydroxyacid synthase II: steady-state intermediate distributions in wild type and mutants by NMR
PMID 15640355doi:10.1073/pnas.0408210101Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 11 records (21 values) from this paper: acetohydroxyacid synthase isozyme II (AHAS II) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetohydroxyacid synthase isozyme II (AHAS II) | Escherichia coli | pyruvatenatural holoenzyme expressed without fusion | 23.3 | 5.2 | 4.49 | pH 7.6 | Open record: acetohydroxyacid synthase isozyme II (AHAS II), pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II) | Escherichia coli | pyruvateN-terminal hexahistidine fusion (His'-, pRSET leader) | 19.8 | 6 | 3.305 | pH 7.6 | Open record: acetohydroxyacid synthase isozyme II (AHAS II), pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant M250A | Escherichia coli | 2-ketobutyrate | 25 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, 2-ketobutyrate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant M250A | Escherichia coli | pyruvate | 3.03 | 36 | 0.084 | pH 7.6 | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant M250A | Escherichia coli | pyruvate | 3 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant R276K | Escherichia coli | 2-ketobutyrate | 9.1 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, 2-ketobutyrate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant R276K | Escherichia coli | pyruvate | 3.27 | 38 | 0.085 | pH 7.6 | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant R276K | Escherichia coli | pyruvate | 4.6 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant W464L | Escherichia coli | 2-ketobutyrate | 13 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, 2-ketobutyrate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant W464L | Escherichia coli | pyruvate | 13.9 | 11.2 | 1.24 | pH 7.6 | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, pyruvate |
| acetohydroxyacid synthase isozyme II (AHAS II)variant W464L | Escherichia coli | pyruvate | 13 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, pyruvate |