EC 2.2.1.6: acetolactate synthase

TransferasesOpen in Browse

CatLog read 62 records for EC 2.2.1.6 from 5 papers, for 3 organisms. Names in these papers: acetohydroxyacid synthase isozyme II (AHAS II), acetohydroxyacid synthase isozyme I (AHAS I), acetolactate synthase (AlsS), acetohydroxyacid synthase (AHAS).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 181 records for EC 2.2.1.6 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.2.1.6 in Browse.

Papers

Papers with records for EC 2.2.1.6
Paper	Organisms	Records
Many of the functional differences between acetohydroxyacid synthase (AHAS) isozyme I and other AHASs are a result of the rapid formation and breakdown of the covalent acetolactate-thiamin…	Escherichia coli	20
Role of a conserved arginine in the mechanism of acetohydroxyacid synthase: catalysis of condensation with a specific ketoacid substrate	Escherichia coli	13
The carboligation reaction of acetohydroxyacid synthase II: steady-state intermediate distributions in wild type and mutants by NMR	Escherichia coli	11
Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli	Bacillus subtilis	10
Engineering Herbicide-Tolerance Rice Expressing an Acetohydroxyacid Synthase with a Single Amino Acid Deletion	Oryza sativa (japonica rice)	8

Records

Records for EC 2.2.1.6 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
acetohydroxyacid synthase (AHAS)	Oryza sativa (japonica rice)	pyruvate	6.7	9.2	0.71	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS), pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvateno herbicide	6.6	9.4	0.7	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvatewith 100 µM herbicide sulfometuron-methyl (SM)	6.6	9.3	0.72	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvatewith 100 µM herbicide rimsulfuron (RS)	6.5	9.4	0.69	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvatewith 100 µM herbicide chlorimuron-methyl (CM)	6.5	9.2	0.71	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvatewith 100 µM herbicide flucarbazone-sodium (FC)	5.9	9.4	0.63	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvatewith 100 µM herbicide bispyribac-sodium (BS)	5.8	11.3	0.51	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase (AHAS)variant ΔW548	Oryza sativa (japonica rice)	pyruvatewith 100 µM herbicide imazethapyr (IT)	5.1	12.1	0.42	pH 7.5, 37 °C	PMID 32070060	Open record: acetohydroxyacid synthase (AHAS) variant ΔW548, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)	Escherichia coli	pyruvatesteady-state AL formation from 2 pyruvate; 10 mM MgCl2	71.3	4.8	14.9	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I), pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)	Escherichia coli	pyruvaterapid mixing-quench NMR experiment; Table 1	64	—	—	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I), pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)	Escherichia coli	pyruvaterapid mixing-quench NMR experiment; Table 1	64	—	—	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I), pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant C83A	Escherichia coli	pyruvate	20.2	1.5	13.5	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant C83A, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant C83S	Escherichia coli	pyruvate	22.6	1.2	18.8	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant C83S, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant C83T	Escherichia coli	pyruvate	2.02	0.33	6.1	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant C83T, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant E60A	Escherichia coli	pyruvate	2	28	0.072	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant E60A, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant E60Q	Escherichia coli	pyruvate	2.1	21	0.102	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant E60Q, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant L476M	Escherichia coli	pyruvatesteady-state AL formation from 2 pyruvate; 10 mM MgCl2	65.3	13	5.03	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant L476M	Escherichia coli	pyruvaterapid mixing-quench NMR experiment; Table 1	58	—	—	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant L476M	Escherichia coli	pyruvaterapid mixing-quench NMR experiment; Table 1	58	—	—	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant L476M/Q480W	Escherichia coli	pyruvate	24.9	3.6	6.93	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M/Q480W, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant M263A	Escherichia coli	pyruvate	61.8	26	2.38	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant M263A, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant Q480W	Escherichia coli	pyruvatesteady-state AL formation from 2 pyruvate; 10 mM MgCl2	15.4	4.7	3.29	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant Q480W, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant Q480W	Escherichia coli	pyruvaterapid mixing-quench NMR experiment; Table 1	14	—	—	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant Q480W, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant Q480W	Escherichia coli	pyruvaterapid mixing-quench NMR experiment; Table 1	9	—	—	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant Q480W, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant R289K	Escherichia coli	pyruvate	85.5	50	1.71	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant R289K, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant R289Q	Escherichia coli	pyruvate	6.4	124	0.052	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant R289Q, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant V391A	Escherichia coli	pyruvate	8.2	17.9	0.46	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant V391A, pyruvate
acetohydroxyacid synthase isozyme I (AHAS I)variant V477I	Escherichia coli	pyruvate	9.5	8	1.19	pH 7.6, 37 °C	PMID 22443469	Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant V477I, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)	Escherichia coli	pyruvate	—	—	6.6	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II), pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)	Escherichia coli	pyruvatenatural holoenzyme expressed without fusion	23.3	5.2	4.49	pH 7.6	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II), pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)	Escherichia coli	pyruvateN-terminal hexahistidine fusion (His'-, pRSET leader)	19.8	6	3.305	pH 7.6	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II), pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant F109L	Escherichia coli	pyruvate	—	—	0.056	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant F109L, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant F109M	Escherichia coli	pyruvate	—	—	0.14	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant F109M, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant F109W	Escherichia coli	pyruvate	—	—	0.036	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant F109W, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant M250A	Escherichia coli	2-ketobutyrate	25	—	—	pH 7.6, 37 °C	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, 2-ketobutyrate
acetohydroxyacid synthase isozyme II (AHAS II)variant M250A	Escherichia coli	pyruvate	—	—	0.13	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant M250A	Escherichia coli	pyruvate	3.03	36	0.084	pH 7.6	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant M250A	Escherichia coli	pyruvate	3	—	—	pH 7.6, 37 °C	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant M250A, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276F	Escherichia coli	pyruvate	—	—	0.014	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276F, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276K	Escherichia coli	2-ketobutyrate	9.1	—	—	pH 7.6, 37 °C	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, 2-ketobutyrate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276K	Escherichia coli	pyruvate	—	—	0.17	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276K	Escherichia coli	pyruvate	3.27	38	0.085	pH 7.6	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276K	Escherichia coli	pyruvate	4.6	—	—	pH 7.6, 37 °C	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276K, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276M	Escherichia coli	pyruvate	—	—	0.011	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276M, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant R276Q	Escherichia coli	pyruvate	—	—	0.008	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant R276Q, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464A	Escherichia coli	pyruvate	—	—	0.691	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464A, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464L	Escherichia coli	2-ketobutyrate	13	—	—	pH 7.6, 37 °C	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, 2-ketobutyrate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464L	Escherichia coli	pyruvate	—	—	2.5	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464L	Escherichia coli	pyruvate	13.9	11.2	1.24	pH 7.6	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464L	Escherichia coli	pyruvate	13	—	—	pH 7.6, 37 °C	PMID 15640355	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464L, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464Q	Escherichia coli	pyruvate	—	—	0.69	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464Q, pyruvate
acetohydroxyacid synthase isozyme II (AHAS II)variant W464Y	Escherichia coli	pyruvate	—	—	1.75	pH 7.6	PMID 15044456	Open record: acetohydroxyacid synthase isozyme II (AHAS II) variant W464Y, pyruvate
acetolactate synthase (AlsS)	Bacillus subtilis	2-ketoisovalerate	8.9	300	0.03	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS), 2-ketoisovalerate
acetolactate synthase (AlsS)	Bacillus subtilis	pyruvate	121	13.6	8.9	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS), pyruvate
acetolactate synthase (AlsS)variant Q487A	Bacillus subtilis	2-ketoisovalerate	1.1	186	0.006	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487A, 2-ketoisovalerate
acetolactate synthase (AlsS)variant Q487A	Bacillus subtilis	pyruvate	58	8.7	6.7	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487A, pyruvate
acetolactate synthase (AlsS)variant Q487G	Bacillus subtilis	2-ketoisovalerate	0.8	175	0.005	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487G, 2-ketoisovalerate
acetolactate synthase (AlsS)variant Q487G	Bacillus subtilis	pyruvate	11	1.6	6.9	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487G, pyruvate
acetolactate synthase (AlsS)variant Q487I	Bacillus subtilis	2-ketoisovalerate	4.8	323	0.01	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487I, 2-ketoisovalerate
acetolactate synthase (AlsS)variant Q487L	Bacillus subtilis	2-ketoisovalerate	5.4	342	0.02	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487L, 2-ketoisovalerate
acetolactate synthase (AlsS)variant Q487S	Bacillus subtilis	2-ketoisovalerate	0.8	154	0.005	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487S, 2-ketoisovalerate
acetolactate synthase (AlsS)variant Q487S	Bacillus subtilis	pyruvate	11	1.1	10	pH 7, 37 °C	PMID 19684168	Open record: acetolactate synthase (AlsS) variant Q487S, pyruvate