Values CatLog read from this paper
Acetolactate synthase from Bacillus subtilis serves as a 2-ketoisovalerate decarboxylase for isobutanol biosynthesis in Escherichia coli
PMID 19684168doi:10.1128/aem.01160-09Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: acetolactate synthase (AlsS) from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetolactate synthase (AlsS) | Bacillus subtilis | 2-ketoisovalerate | 8.9 | 300 | 0.03 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS), 2-ketoisovalerate |
| acetolactate synthase (AlsS) | Bacillus subtilis | pyruvate | 121 | 13.6 | 8.9 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS), pyruvate |
| acetolactate synthase (AlsS)variant Q487A | Bacillus subtilis | 2-ketoisovalerate | 1.1 | 186 | 0.006 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487A, 2-ketoisovalerate |
| acetolactate synthase (AlsS)variant Q487A | Bacillus subtilis | pyruvate | 58 | 8.7 | 6.7 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487A, pyruvate |
| acetolactate synthase (AlsS)variant Q487G | Bacillus subtilis | 2-ketoisovalerate | 0.8 | 175 | 0.005 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487G, 2-ketoisovalerate |
| acetolactate synthase (AlsS)variant Q487G | Bacillus subtilis | pyruvate | 11 | 1.6 | 6.9 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487G, pyruvate |
| acetolactate synthase (AlsS)variant Q487I | Bacillus subtilis | 2-ketoisovalerate | 4.8 | 323 | 0.01 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487I, 2-ketoisovalerate |
| acetolactate synthase (AlsS)variant Q487L | Bacillus subtilis | 2-ketoisovalerate | 5.4 | 342 | 0.02 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487L, 2-ketoisovalerate |
| acetolactate synthase (AlsS)variant Q487S | Bacillus subtilis | 2-ketoisovalerate | 0.8 | 154 | 0.005 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487S, 2-ketoisovalerate |
| acetolactate synthase (AlsS)variant Q487S | Bacillus subtilis | pyruvate | 11 | 1.1 | 10 | pH 7, 37 °C | Open record: acetolactate synthase (AlsS) variant Q487S, pyruvate |