Values CatLog read from this paper
Many of the functional differences between acetohydroxyacid synthase (AHAS) isozyme I and other AHASs are a result of the rapid formation and breakdown of the covalent acetolactate-thiamin…
PMID 22443469doi:10.1111/j.1742-4658.2012.08577.xOpen in Browse
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CatLog read 20 records (48 values) from this paper: acetohydroxyacid synthase isozyme I (AHAS I) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| acetohydroxyacid synthase isozyme I (AHAS I) | Escherichia coli | pyruvatesteady-state AL formation from 2 pyruvate; 10 mM MgCl2 | 71.3 | 4.8 | 14.9 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I), pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I) | Escherichia coli | pyruvaterapid mixing-quench NMR experiment; Table 1 | 64 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I), pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I) | Escherichia coli | pyruvaterapid mixing-quench NMR experiment; Table 1 | 64 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I), pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant C83A | Escherichia coli | pyruvate | 20.2 | 1.5 | 13.5 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant C83A, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant C83S | Escherichia coli | pyruvate | 22.6 | 1.2 | 18.8 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant C83S, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant C83T | Escherichia coli | pyruvate | 2.02 | 0.33 | 6.1 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant C83T, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant E60A | Escherichia coli | pyruvate | 2 | 28 | 0.072 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant E60A, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant E60Q | Escherichia coli | pyruvate | 2.1 | 21 | 0.102 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant E60Q, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant L476M | Escherichia coli | pyruvatesteady-state AL formation from 2 pyruvate; 10 mM MgCl2 | 65.3 | 13 | 5.03 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant L476M | Escherichia coli | pyruvaterapid mixing-quench NMR experiment; Table 1 | 58 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant L476M | Escherichia coli | pyruvaterapid mixing-quench NMR experiment; Table 1 | 58 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant L476M/Q480W | Escherichia coli | pyruvate | 24.9 | 3.6 | 6.93 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant L476M/Q480W, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant M263A | Escherichia coli | pyruvate | 61.8 | 26 | 2.38 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant M263A, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant Q480W | Escherichia coli | pyruvatesteady-state AL formation from 2 pyruvate; 10 mM MgCl2 | 15.4 | 4.7 | 3.29 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant Q480W, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant Q480W | Escherichia coli | pyruvaterapid mixing-quench NMR experiment; Table 1 | 14 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant Q480W, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant Q480W | Escherichia coli | pyruvaterapid mixing-quench NMR experiment; Table 1 | 9 | — | — | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant Q480W, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant R289K | Escherichia coli | pyruvate | 85.5 | 50 | 1.71 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant R289K, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant R289Q | Escherichia coli | pyruvate | 6.4 | 124 | 0.052 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant R289Q, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant V391A | Escherichia coli | pyruvate | 8.2 | 17.9 | 0.46 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant V391A, pyruvate |
| acetohydroxyacid synthase isozyme I (AHAS I)variant V477I | Escherichia coli | pyruvate | 9.5 | 8 | 1.19 | pH 7.6, 37 °C | Open record: acetohydroxyacid synthase isozyme I (AHAS I) variant V477I, pyruvate |