Values CatLog read from this paper
Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site
PMID 15654896doi:10.1111/j.1742-4658.2004.04506.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 44 records (62 values) from this paper: ketol-acid reductoisomerase (KARI) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 2-acetolactate | 2.231 | 0.25 | 9.02 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 2-acetolactate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 2-Ketobutyrate | 0.167 | 4.56 | 0.036 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 2-Ketobutyrate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 2-Ketoisovalerate | 0.182 | 6.91 | 0.026 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 2-Ketoisovalerate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 2-Ketopantoate | 0.194 | 0.17 | 1.104 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 2-Ketopantoate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 2-Ketovalerate | 0.05 | 3.15 | 0.016 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 2-Ketovalerate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 3-Hydroxy-2-ketobutyrate | 0.594 | 0.21 | 2.829 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 3-Hydroxy-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 3-Hydroxy-3-methyl-2-ketobutyrate | 3.511 | 0.27 | 13.20 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 3-Hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | 3-hydroxypyruvate | 5.376 | 2.96 | 1.818 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | NADPH | — | 0.00253 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), NADPH |
| ketol-acid reductoisomerase (KARI) | Escherichia coli | Pyruvate | 0.021 | 1.54 | 0.014 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI), Pyruvate |
| ketol-acid reductoisomerase (KARI)variant D217N | Escherichia coli | 3-hydroxy-3-methyl-2-ketobutyrate | — | 7.64 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant D217N, 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI)variant D217N | Escherichia coli | NADPH | — | 0.00508 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant D217N, NADPH |
| ketol-acid reductoisomerase (KARI)variant E213D | Escherichia coli | 2-acetolactate | — | 0.922 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E213D, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant E213D | Escherichia coli | 3-hydroxypyruvate | — | 3.67 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E213D, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant E213D | Escherichia coli | NADPH | — | 0.016 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E213D, NADPH |
| ketol-acid reductoisomerase (KARI)variant E213Q | Escherichia coli | 3-hydroxypyruvate | — | 0.441 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E213Q, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant E221D | Escherichia coli | 2-acetolactate | — | 0.356 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E221D, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant E221D | Escherichia coli | 3-hydroxypyruvate | — | 1.37 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E221D, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant E221D | Escherichia coli | NADPH | — | 0.0203 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E221D, NADPH |
| ketol-acid reductoisomerase (KARI)variant E389D | Escherichia coli | 2-acetolactate | — | 2.028 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E389D, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant E389D | Escherichia coli | 3-hydroxypyruvate | — | 8.5 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E389D, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant E389D | Escherichia coli | NADPH | — | 0.023 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E389D, NADPH |
| ketol-acid reductoisomerase (KARI)variant E389Q | Escherichia coli | 3-hydroxypyruvate | — | 8.88 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E389Q, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant E393D | Escherichia coli | 3-hydroxypyruvate | — | 3.32 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E393D, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant E393D | Escherichia coli | NADPH | — | 0.00476 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E393D, NADPH |
| ketol-acid reductoisomerase (KARI)variant E393Q | Escherichia coli | 3-hydroxypyruvate | — | 0.588 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant E393Q, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant H132K | Escherichia coli | 3-hydroxypyruvate | — | 0.818 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant H132K, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant H132K | Escherichia coli | NADPH | — | 0.00312 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant H132K, NADPH |
| ketol-acid reductoisomerase (KARI)variant H132Q | Escherichia coli | 2-acetolactate | — | 0.929 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant H132Q, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant H132Q | Escherichia coli | 3-hydroxypyruvate | — | 7.43 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant H132Q, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant H132Q | Escherichia coli | NADPH | — | 0.0696 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant H132Q, NADPH |
| ketol-acid reductoisomerase (KARI)variant K155E | Escherichia coli | 3-hydroxypyruvate | — | 2.66 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155E, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant K155E | Escherichia coli | NADPH | — | 0.00804 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155E, NADPH |
| ketol-acid reductoisomerase (KARI)variant K155Q | Escherichia coli | 3-hydroxypyruvate | — | 15.3 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155Q, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant K155Q | Escherichia coli | NADPH | — | 0.0093 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155Q, NADPH |
| ketol-acid reductoisomerase (KARI)variant K155R | Escherichia coli | 2-acetolactate | — | 1.218 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155R, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant K155R | Escherichia coli | 3-hydroxypyruvate | — | 13.6 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155R, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant K155R | Escherichia coli | NADPH | — | 0.00727 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant K155R, NADPH |
| ketol-acid reductoisomerase (KARI)variant S414A | Escherichia coli | 2-acetolactate | — | 0.711 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant S414A, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant S414A | Escherichia coli | 3-hydroxypyruvate | — | 0.334 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant S414A, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant S414A | Escherichia coli | NADPH | — | 0.00837 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant S414A, NADPH |
| ketol-acid reductoisomerase (KARI)variant S414T | Escherichia coli | 2-acetolactate | — | 0.414 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant S414T, 2-acetolactate |
| ketol-acid reductoisomerase (KARI)variant S414T | Escherichia coli | 3-hydroxypyruvate | — | 1.101 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant S414T, 3-hydroxypyruvate |
| ketol-acid reductoisomerase (KARI)variant S414T | Escherichia coli | NADPH | — | 0.00507 | — | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (KARI) variant S414T, NADPH |