EC 1.1.1.86: ketol-acid reductoisomerase (NADP+)

OxidoreductasesOpen in Browse

CatLog read 187 records for EC 1.1.1.86 from 7 papers, for 11 organisms. Names in these papers: ketol-acid reductoisomerase (KARI), ketol acid reductoisomerase (KARI), ketol-acid reductoisomerase (KARI, IlvC), ketol-acid reductoisomerase (Sac-KARI) and 13 more.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 110 records for EC 1.1.1.86 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.1.86 in Browse.

Papers

Papers with records for EC 1.1.1.86
Paper	Organisms	Records
Molecular annotation of ketol-acid reductoisomerases from Streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity	Corynebacterium glutamicum, Escherichia coli and 7 more	81
Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site	Escherichia coli	44
Reversal of the nucleotide specificity of ketol acid reductoisomerase by site-directed mutagenesis identifies the NADPH binding site	Escherichia coli	17
Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli	Escherichia coli	14
NADH/NADPH bi-cofactor-utilizing and thermoactive ketol-acid reductoisomerase from Sulfolobus acidocaldarius	Sulfolobus acidocaldarius	14
doi:10.3390/cryst9110551	Streptococcus pneumoniae	14
Crystal Structure and Biochemical Characterization of Ketol-Acid Reductoisomerase from Corynebacterium glutamicum	Corynebacterium glutamicum	3

Records

Records for EC 1.1.1.86 read from the paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Paper	Open record
ketol acid reductoisomerase (KARI)	Escherichia coli	NADH	0.0018	0.206	0.00873	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI), NADH
ketol acid reductoisomerase (KARI)	Escherichia coli	NADP	0.052	0.0042	12.3	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI), NADP
ketol acid reductoisomerase (KARI)	Escherichia coli	NADPH	0.12	0.0073	16.5	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI), NADPH
ketol acid reductoisomerase (KARI)variant K69L	Escherichia coli	NADP	3.2 × 10⁻⁴	0.0089	0.035	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant K69L, NADP
ketol acid reductoisomerase (KARI)variant K69L	Escherichia coli	NADPH	0.212	0.0076	28	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant K69L, NADPH
ketol acid reductoisomerase (KARI)variant K75Q	Escherichia coli	NADP	6.7 × 10⁻⁴	0.0112	0.06	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant K75Q, NADP
ketol acid reductoisomerase (KARI)variant K75Q	Escherichia coli	NADPH	0.0093	0.0025	3.8	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant K75Q, NADPH
ketol acid reductoisomerase (KARI)variant R68D/K69L/K75V/R76D	Escherichia coli	NADH	0.088	0.019	4.5	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R68D/K69L/K75V/R76D, NADH
ketol acid reductoisomerase (KARI)variant R68D/K69L/K75V/R76D	Escherichia coli	NADPH	0.0328	0.222	0.148	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R68D/K69L/K75V/R76D, NADPH
ketol acid reductoisomerase (KARI)variant R68Q	Escherichia coli	NADP	1.5 × 10⁻⁴	0.029	0.0052	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R68Q, NADP
ketol acid reductoisomerase (KARI)variant R68Q	Escherichia coli	NADPH	0.0067	0.0245	0.27	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R68Q, NADPH
ketol acid reductoisomerase (KARI)variant R76D	Escherichia coli	NADH	0.088	0.082	1.08	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R76D, NADH
ketol acid reductoisomerase (KARI)variant R76D	Escherichia coli	NADPH	0.03	0.401	0.075	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R76D, NADPH
ketol acid reductoisomerase (KARI)variant R76Q	Escherichia coli	NADP	2 × 10⁻⁴	0.072	0.0028	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R76Q, NADP
ketol acid reductoisomerase (KARI)variant R76Q	Escherichia coli	NADPH	0.0062	0.0365	0.17	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R76Q, NADPH
ketol acid reductoisomerase (KARI)variant R76Q/R68A	Escherichia coli	NADH	0.037	0.193	18	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R76Q/R68A, NADH
ketol acid reductoisomerase (KARI)variant R76Q/R68A	Escherichia coli	NADPH	0.0043	0.16	0.027	pH 8, 22 °C	PMID 9015391	Open record: ketol acid reductoisomerase (KARI) variant R76Q/R68A, NADPH
ketol-acid reductoisomerase (CgKARI)	Corynebacterium glutamicum	(S)-2-acetolactate	0.2348	2.161	0.1087	pH 8	PMID 31298526	Open record: ketol-acid reductoisomerase (CgKARI), (S)-2-acetolactate
ketol-acid reductoisomerase (CgKARI)	Corynebacterium glutamicum	NADH	0.002747	0.05353	0.02995	pH 8	PMID 31298526	Open record: ketol-acid reductoisomerase (CgKARI), NADH
ketol-acid reductoisomerase (CgKARI)	Corynebacterium glutamicum	NADPH	0.2176	0.02258	9.638	pH 8	PMID 31298526	Open record: ketol-acid reductoisomerase (CgKARI), NADPH
ketol-acid reductoisomerase (KARI)	Escherichia coli	2-acetolactate	2.231	0.25	9.02	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 2-acetolactate
ketol-acid reductoisomerase (KARI)	Escherichia coli	2-Ketobutyrate	0.167	4.56	0.036	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 2-Ketobutyrate
ketol-acid reductoisomerase (KARI)	Escherichia coli	2-Ketoisovalerate	0.182	6.91	0.026	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 2-Ketoisovalerate
ketol-acid reductoisomerase (KARI)	Escherichia coli	2-Ketopantoate	0.194	0.17	1.104	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 2-Ketopantoate
ketol-acid reductoisomerase (KARI)	Escherichia coli	2-Ketovalerate	0.05	3.15	0.016	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 2-Ketovalerate
ketol-acid reductoisomerase (KARI)	Escherichia coli	3-Hydroxy-2-ketobutyrate	0.594	0.21	2.829	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 3-Hydroxy-2-ketobutyrate
ketol-acid reductoisomerase (KARI)	Escherichia coli	3-Hydroxy-3-methyl-2-ketobutyrate	3.511	0.27	13.20	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 3-Hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI)	Escherichia coli	3-hydroxypyruvate	5.376	2.96	1.818	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)	Escherichia coli	NADPH	—	0.00253	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), NADPH
ketol-acid reductoisomerase (KARI)	Escherichia coli	Pyruvate	0.021	1.54	0.014	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI), Pyruvate
ketol-acid reductoisomerase (KARI)variant D217N	Escherichia coli	3-hydroxy-3-methyl-2-ketobutyrate	—	7.64	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant D217N, 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI)variant D217N	Escherichia coli	NADPH	—	0.00508	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant D217N, NADPH
ketol-acid reductoisomerase (KARI)variant E213D	Escherichia coli	2-acetolactate	—	0.922	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E213D, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant E213D	Escherichia coli	3-hydroxypyruvate	—	3.67	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E213D, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant E213D	Escherichia coli	NADPH	—	0.016	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E213D, NADPH
ketol-acid reductoisomerase (KARI)variant E213Q	Escherichia coli	3-hydroxypyruvate	—	0.441	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E213Q, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant E221D	Escherichia coli	2-acetolactate	—	0.356	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E221D, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant E221D	Escherichia coli	3-hydroxypyruvate	—	1.37	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E221D, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant E221D	Escherichia coli	NADPH	—	0.0203	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E221D, NADPH
ketol-acid reductoisomerase (KARI)variant E389D	Escherichia coli	2-acetolactate	—	2.028	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E389D, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant E389D	Escherichia coli	3-hydroxypyruvate	—	8.5	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E389D, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant E389D	Escherichia coli	NADPH	—	0.023	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E389D, NADPH
ketol-acid reductoisomerase (KARI)variant E389Q	Escherichia coli	3-hydroxypyruvate	—	8.88	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E389Q, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant E393D	Escherichia coli	3-hydroxypyruvate	—	3.32	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E393D, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant E393D	Escherichia coli	NADPH	—	0.00476	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E393D, NADPH
ketol-acid reductoisomerase (KARI)variant E393Q	Escherichia coli	3-hydroxypyruvate	—	0.588	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant E393Q, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant H132K	Escherichia coli	3-hydroxypyruvate	—	0.818	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant H132K, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant H132K	Escherichia coli	NADPH	—	0.00312	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant H132K, NADPH
ketol-acid reductoisomerase (KARI)variant H132Q	Escherichia coli	2-acetolactate	—	0.929	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant H132Q, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant H132Q	Escherichia coli	3-hydroxypyruvate	—	7.43	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant H132Q, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant H132Q	Escherichia coli	NADPH	—	0.0696	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant H132Q, NADPH
ketol-acid reductoisomerase (KARI)variant K155E	Escherichia coli	3-hydroxypyruvate	—	2.66	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155E, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant K155E	Escherichia coli	NADPH	—	0.00804	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155E, NADPH
ketol-acid reductoisomerase (KARI)variant K155Q	Escherichia coli	3-hydroxypyruvate	—	15.3	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155Q, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant K155Q	Escherichia coli	NADPH	—	0.0093	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155Q, NADPH
ketol-acid reductoisomerase (KARI)variant K155R	Escherichia coli	2-acetolactate	—	1.218	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155R, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant K155R	Escherichia coli	3-hydroxypyruvate	—	13.6	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155R, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant K155R	Escherichia coli	NADPH	—	0.00727	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant K155R, NADPH
ketol-acid reductoisomerase (KARI)variant S414A	Escherichia coli	2-acetolactate	—	0.711	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant S414A, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant S414A	Escherichia coli	3-hydroxypyruvate	—	0.334	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant S414A, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant S414A	Escherichia coli	NADPH	—	0.00837	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant S414A, NADPH
ketol-acid reductoisomerase (KARI)variant S414T	Escherichia coli	2-acetolactate	—	0.414	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant S414T, 2-acetolactate
ketol-acid reductoisomerase (KARI)variant S414T	Escherichia coli	3-hydroxypyruvate	—	1.101	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant S414T, 3-hydroxypyruvate
ketol-acid reductoisomerase (KARI)variant S414T	Escherichia coli	NADPH	—	0.00507	—	pH 8, 37 °C	PMID 15654896	Open record: ketol-acid reductoisomerase (KARI) variant S414T, NADPH
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	2-hydroxy-2-ethyl-3-ketobutyrate	0.9	75	0.012	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	2-hydroxy-2-methyl-3-ketobutyrate	3.2	8	0.4	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	2-methylacetoacetate	0.4	40	0.01	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), 2-methylacetoacetate
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	3-hydroxy-3-ethyl-2-ketobutyrate	0.18	5	0.036	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	3-hydroxy-3-methyl-2-ketobutyrate	0.9	2.3	0.391	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	hydroxypyruvate	0.5	40	0.06	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), hydroxypyruvate
ketol-acid reductoisomerase (KARI, Cgl)	Corynebacterium glutamicum	pyruvate	8.6	1.07 × 10³	0.008	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Cgl), pyruvate
ketol-acid reductoisomerase (KARI, Eco)	Escherichia coli	2-hydroxy-2-ethyl-3-ketobutyrate	3.5	0.3	11.7	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Eco), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Eco)	Escherichia coli	2-hydroxy-2-methyl-3-ketobutyrate	2	0.28	7.142	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Eco), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Eco)	Escherichia coli	3-hydroxy-3-ethyl-2-ketobutyrate	3.8	0.29	13.10	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Eco), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Eco)	Escherichia coli	3-hydroxy-3-methyl-2-ketobutyrate	3.1	0.26	11.92	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Eco), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, IlvC)	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	0.3	1.08	0.3	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC), nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	3.6	0.04	87.3	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC), nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110A	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	2.6	0.04	60.3	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110A, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110A	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	0.6	0.57	1.1	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110A, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	2.3	0.03	74	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110V, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	0.2	0.65	0.4	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110V, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110A	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	3.1	0.28	11	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110A, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110A	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	7.2	0.024	302	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110A, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110V	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	3.3	0.14	24.8	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110V, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110V	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	7.2	0.013	569	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110V, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	3.6	0.13	27.6	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	2.5	0.66	3.8	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	2	0.065	31.8	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D/Q110V, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	2.2	0.065	34.2	pH 7	PMID 21515217	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D/Q110V, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	2-hydroxy-2-ethyl-3-ketobutyrate	1.3	6.7	0.194	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	2-hydroxy-2-methyl-3-ketobutyrate	0.63	2	0.315	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	2-methylacetoacetate	1.2	40	0.03	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), 2-methylacetoacetate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	3-hydroxy-3-ethyl-2-ketobutyrate	0.9	20	0.045	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	3-hydroxy-3-methyl-2-ketobutyrate	0.8	4	0.2	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	hydroxypyruvate	1	50	0.02	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), hydroxypyruvate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	pyrroline-5-carboxylate	—	—	0.0055	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), pyrroline-5-carboxylate
ketol-acid reductoisomerase (KARI, Sam)	Streptomyces ambofaciens	pyruvate	6.3	1.05 × 10³	0.006	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sam), pyruvate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	2-hydroxy-2-ethyl-3-ketobutyrate	0.4	2.6	0.153	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	2-hydroxy-2-methyl-3-ketobutyrate	0.24	0.8	0.3	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	2-methylacetoacetate	1.8	30	0.06	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), 2-methylacetoacetate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	3-hydroxy-3-ethyl-2-ketobutyrate	0.8	40	0.02	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	3-hydroxy-3-methyl-2-ketobutyrate	1.2	13	0.52	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	hydroxypyruvate	2	60	0.05	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), hydroxypyruvate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	pyrroline-5-carboxylate	—	—	0.0056	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), pyrroline-5-carboxylate
ketol-acid reductoisomerase (KARI, Sav)	Streptomyces avermitilis	pyruvate	3.6	1.2 × 10³	0.003	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sav), pyruvate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	2-hydroxy-2-ethyl-3-ketobutyrate	0.44	2.5	0.176	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	2-hydroxy-2-methyl-3-ketobutyrate	0.42	1.2	0.35	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	2-methylacetoacetate	1.05	35	0.03	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), 2-methylacetoacetate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	3-hydroxy-3-ethyl-2-ketobutyrate	1	35	0.028	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	3-hydroxy-3-methyl-2-ketobutyrate	0.5	8	0.85	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	hydroxypyruvate	1.5	30	0.04	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), hydroxypyruvate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	pyrroline-5-carboxylate	—	—	0.0024	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), pyrroline-5-carboxylate
ketol-acid reductoisomerase (KARI, Sgr)	Streptomyces griseus	pyruvate	1	1.06 × 10³	0.001	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Sgr), pyruvate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	2-hydroxy-2-ethyl-3-ketobutyrate	0.18	10	0.018	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	2-hydroxy-2-methyl-3-ketobutyrate	3.6	9	0.4	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	2-methylacetoacetate	2	40	0.05	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), 2-methylacetoacetate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	3-hydroxy-3-ethyl-2-ketobutyrate	1.4	50	0.028	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	3-hydroxy-3-methyl-2-ketobutyrate	5.2	8.6	0.53	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	hydroxypyruvate	2	60	0.03	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), hydroxypyruvate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	pyrroline-5-carboxylate	—	—	0.0028	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), pyrroline-5-carboxylate
ketol-acid reductoisomerase (KARI, Spr)	Streptomyces pristinaespiralis	pyruvate	3	1.08 × 10³	0.003	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase (KARI, Spr), pyruvate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate2 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH	0.058	0.184	0.32	pH 8, 25 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate10 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH	0.068	0.14	0.49	pH 8, 25 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate2 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH	0.192	0.157	1.23	pH 8, 37 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate10 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH	0.195	0.074	2.63	pH 8, 37 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate2 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH	0.957	0.093	10.3	pH 8, 60 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate10 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH	0.876	0.06	14.58	pH 8, 60 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate2 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH	0.055	0.469	0.12	pH 8, 25 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate10 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH	—	0.188	0.33	pH 8, 25 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate2 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH	0.157	0.165	0.95	pH 8, 37 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate10 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH	0.164	0.099	1.66	pH 8, 37 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate2 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH	0.526	0.144	3.66	pH 8, 60 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	(S)-2-acetolactate6 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH	0.444	0.091	4.88	pH 8, 60 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	NADH	0.056	0.006	9.5	pH 8, 25 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), NADH
ketol-acid reductoisomerase (Sac-KARI)	Sulfolobus acidocaldarius	NADPH	0.064	4 × 10⁻⁴	185.8	pH 8, 25 °C	PMID 29739976	Open record: ketol-acid reductoisomerase (Sac-KARI), NADPH
ketol-acid reductoisomerase IlvC (SpIlvC)	Streptococcus pneumoniae	2-acetolactate	0.1	0.07	1.4	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC), 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant D191G	Streptococcus pneumoniae	2-acetolactate	0.15	0.355	0.42	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant D191G, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant D191K	Streptococcus pneumoniae	2-acetolactate	0.22	1.036	0.21	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant D191K, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant D83G	Streptococcus pneumoniae	2-acetolactate	0.13	0.33	0.39	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant D83G, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant E195A	Streptococcus pneumoniae	2-acetolactate	0.12	0.732	0.16	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant E195A, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant E195K	Streptococcus pneumoniae	2-acetolactate	0.18	0.895	0.2	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant E195K, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant E195S	Streptococcus pneumoniae	2-acetolactate	0.25	1.467	0.17	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant E195S, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant R49A	Streptococcus pneumoniae	2-acetolactate	0.18	0.588	0.3	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant R49A, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant R49E	Streptococcus pneumoniae	2-acetolactate	0.18	0.472	0.38	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant R49E, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant R49G	Streptococcus pneumoniae	2-acetolactate	0.16	0.395	0.43	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant R49G, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant S53A	Streptococcus pneumoniae	2-acetolactate	0.17	0.677	0.25	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant S53A, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant S53G	Streptococcus pneumoniae	2-acetolactate	0.11	0.199	0.55	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant S53G, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant S53K	Streptococcus pneumoniae	2-acetolactate	0.14	0.471	0.3	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant S53K, 2-acetolactate
ketol-acid reductoisomerase IlvC (SpIlvC)variant S53T	Streptococcus pneumoniae	2-acetolactate	0.12	0.345	0.34	pH 8, 30 °C	doi:10.3390/cryst9110551	Open record: ketol-acid reductoisomerase IlvC (SpIlvC) variant S53T, 2-acetolactate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	2-hydroxy-2-ethyl-3-ketobutyrate	3.2	67	0.047	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	2-hydroxy-2-methyl-3-ketobutyrate	1.1	1.6	0.687	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	2-methylacetoacetate	1	50	0.02	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), 2-methylacetoacetate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	3-hydroxy-3-ethyl-2-ketobutyrate	1.8	45	0.04	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	3-hydroxy-3-methyl-2-ketobutyrate	2.2	3	0.733	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	pyrroline-5-carboxylate	—	—	0.0024	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), pyrroline-5-carboxylate
ketol-acid reductoisomerase KARI1 (Sco1)	Streptomyces coelicolor	pyruvate	2.8	950	0.003	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sco1), pyruvate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	2-hydroxy-2-ethyl-3-ketobutyrate	3.6	71	0.05	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	2-hydroxy-2-methyl-3-ketobutyrate	0.7	1.2	0.583	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	2-methylacetoacetate	0.675	45	0.015	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), 2-methylacetoacetate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	3-hydroxy-3-ethyl-2-ketobutyrate	1.3	50	0.046	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	3-hydroxy-3-methyl-2-ketobutyrate	2.8	4	0.7	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	pyrroline-5-carboxylate	—	—	0.0037	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), pyrroline-5-carboxylate
ketol-acid reductoisomerase KARI1 (Sli1)	Streptomyces lividans	pyruvate	6.7	1.12 × 10³	0.006	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI1 (Sli1), pyruvate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	2-hydroxy-2-ethyl-3-ketobutyrate	0.39	12	0.032	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	2-hydroxy-2-methyl-3-ketobutyrate	0.24	12	0.02	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	2-methylacetoacetate	0.6	60	0.01	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), 2-methylacetoacetate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	3-hydroxy-3-ethyl-2-ketobutyrate	0.8	20	0.04	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	3-hydroxy-3-methyl-2-ketobutyrate	0.3	10	0.03	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	hydroxypyruvate	0.5	40	0.06	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), hydroxypyruvate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	pyrroline-5-carboxylate	—	—	0.003	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), pyrroline-5-carboxylate
ketol-acid reductoisomerase KARI2 (Sco2)	Streptomyces coelicolor	pyruvate	5.6	700	0.008	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sco2), pyruvate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	2-hydroxy-2-ethyl-3-ketobutyrate	0.12	5	0.048	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	2-hydroxy-2-methyl-3-ketobutyrate	0.28	9.3	0.03	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	2-methylacetoacetate	0.5	50	0.01	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), 2-methylacetoacetate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	3-hydroxy-3-ethyl-2-ketobutyrate	0.9	20	0.046	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	3-hydroxy-3-methyl-2-ketobutyrate	0.4	8.6	0.045	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	hydroxypyruvate	0.7	50	0.04	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), hydroxypyruvate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	pyrroline-5-carboxylate	—	—	0.0061	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), pyrroline-5-carboxylate
ketol-acid reductoisomerase KARI2 (Sli2)	Streptomyces lividans	pyruvate	3	1.01 × 10³	0.003	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Sli2), pyruvate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	2-hydroxy-2-ethyl-3-ketobutyrate	0.3	25	0.012	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), 2-hydroxy-2-ethyl-3-ketobutyrate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	2-hydroxy-2-methyl-3-ketobutyrate	2	1.8	1.111	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), 2-hydroxy-2-methyl-3-ketobutyrate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	2-methylacetoacetate	2	50	0.04	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), 2-methylacetoacetate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	3-hydroxy-3-ethyl-2-ketobutyrate	0.5	50	0.01	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), 3-hydroxy-3-ethyl-2-ketobutyrate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	3-hydroxy-3-methyl-2-ketobutyrate	2.5	5	0.5	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), 3-hydroxy-3-methyl-2-ketobutyrate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	hydroxypyruvate	2	50	0.05	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), hydroxypyruvate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	pyrroline-5-carboxylate	—	—	0.0038	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), pyrroline-5-carboxylate
ketol-acid reductoisomerase KARI2 (Svi2)	Streptomyces viridifaciens	pyruvate	5.7	1.14 × 10³	0.005	pH 8, 25 °C	PMID 25296650	Open record: ketol-acid reductoisomerase KARI2 (Svi2), pyruvate