Values CatLog read from this paper
NADH/NADPH bi-cofactor-utilizing and thermoactive ketol-acid reductoisomerase from Sulfolobus acidocaldarius
PMID 29739976doi:10.1038/s41598-018-25361-4Open in Browse
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CatLog read 14 records (41 values) from this paper: ketol-acid reductoisomerase (Sac-KARI) from Sulfolobus acidocaldarius.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate2 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH | 0.058 | 0.184 | 0.32 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate10 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH | 0.068 | 0.14 | 0.49 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate2 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH | 0.192 | 0.157 | 1.23 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate10 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH | 0.195 | 0.074 | 2.63 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate2 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH | 0.957 | 0.093 | 10.3 | pH 8, 60 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate10 mM MgSO4; saturating NADPH 0.22 mM; cosubstrate NADPH | 0.876 | 0.06 | 14.58 | pH 8, 60 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate2 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH | 0.055 | 0.469 | 0.12 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate10 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH | — | 0.188 | 0.33 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate2 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH | 0.157 | 0.165 | 0.95 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate10 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH | 0.164 | 0.099 | 1.66 | pH 8, 37 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate2 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH | 0.526 | 0.144 | 3.66 | pH 8, 60 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | (S)-2-acetolactate6 mM MgSO4; saturating NADH 0.22 mM; cosubstrate NADH | 0.444 | 0.091 | 4.88 | pH 8, 60 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), (S)-2-acetolactate |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | NADH | 0.056 | 0.006 | 9.5 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), NADH |
| ketol-acid reductoisomerase (Sac-KARI) | Sulfolobus acidocaldarius | NADPH | 0.064 | 4 × 10⁻⁴ | 185.8 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (Sac-KARI), NADPH |