Values CatLog read from this paper
Molecular annotation of ketol-acid reductoisomerases from Streptomyces reveals a novel amino acid biosynthesis interlock mediated by enzyme promiscuity
PMID 25296650doi:10.1111/1751-7915.12175Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 82 records (226 values) from this paper: ketol-acid reductoisomerase (KARI, Sam), ketol-acid reductoisomerase (KARI, Sav), ketol-acid reductoisomerase (KARI, Sgr) and 9 more from Streptomyces coelicolor, Streptomyces lividans, Streptomyces ambofaciens and 6 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.9 | 75 | 0.012 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | 2-hydroxy-2-methyl-3-ketobutyrate | 3.2 | 8 | 0.4 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | 2-methylacetoacetate | 0.4 | 40 | 0.01 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), 2-methylacetoacetate |
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | 3-hydroxy-3-ethyl-2-ketobutyrate | 0.18 | 5 | 0.036 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | 3-hydroxy-3-methyl-2-ketobutyrate | 0.9 | 2.3 | 0.391 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | hydroxypyruvate | 0.5 | 40 | 0.06 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), hydroxypyruvate |
| ketol-acid reductoisomerase (KARI, Cgl) | Corynebacterium glutamicum | pyruvate | 8.6 | 1.07 × 10³ | 0.008 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Cgl), pyruvate |
| ketol-acid reductoisomerase (KARI, Eco) | Escherichia coli | 2-hydroxy-2-ethyl-3-ketobutyrate | 3.5 | 0.3 | 11.7 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Eco), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Eco) | Escherichia coli | 2-hydroxy-2-methyl-3-ketobutyrate | 2 | 0.28 | 7.142 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Eco), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Eco) | Escherichia coli | 3-hydroxy-3-ethyl-2-ketobutyrate | 3.8 | 0.29 | 13.10 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Eco), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Eco) | Escherichia coli | 3-hydroxy-3-methyl-2-ketobutyrate | 3.1 | 0.26 | 11.92 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Eco), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | 2-hydroxy-2-ethyl-3-ketobutyrate | 1.3 | 6.7 | 0.194 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | 2-hydroxy-2-methyl-3-ketobutyrate | 0.63 | 2 | 0.315 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | 2-methylacetoacetate | 1.2 | 40 | 0.03 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), 2-methylacetoacetate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | 3-hydroxy-3-ethyl-2-ketobutyrate | 0.9 | 20 | 0.045 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | 3-hydroxy-3-methyl-2-ketobutyrate | 0.8 | 4 | 0.2 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | hydroxypyruvate | 1 | 50 | 0.02 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), hydroxypyruvate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | pyrroline-5-carboxylate | — | — | 0.0055 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase (KARI, Sam) | Streptomyces ambofaciens | pyruvate | 6.3 | 1.05 × 10³ | 0.006 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sam), pyruvate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.4 | 2.6 | 0.153 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | 2-hydroxy-2-methyl-3-ketobutyrate | 0.24 | 0.8 | 0.3 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | 2-methylacetoacetate | 1.8 | 30 | 0.06 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), 2-methylacetoacetate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | 3-hydroxy-3-ethyl-2-ketobutyrate | 0.8 | 40 | 0.02 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | 3-hydroxy-3-methyl-2-ketobutyrate | 1.2 | 13 | 0.52 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | hydroxypyruvate | 2 | 60 | 0.05 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), hydroxypyruvate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | pyrroline-5-carboxylate | — | — | 0.0056 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase (KARI, Sav) | Streptomyces avermitilis | pyruvate | 3.6 | 1.2 × 10³ | 0.003 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sav), pyruvate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.44 | 2.5 | 0.176 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | 2-hydroxy-2-methyl-3-ketobutyrate | 0.42 | 1.2 | 0.35 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | 2-methylacetoacetate | 1.05 | 35 | 0.03 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), 2-methylacetoacetate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | 3-hydroxy-3-ethyl-2-ketobutyrate | 1 | 35 | 0.028 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | 3-hydroxy-3-methyl-2-ketobutyrate | 0.5 | 8 | 0.85 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | hydroxypyruvate | 1.5 | 30 | 0.04 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), hydroxypyruvate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | pyrroline-5-carboxylate | — | — | 0.0024 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase (KARI, Sgr) | Streptomyces griseus | pyruvate | 1 | 1.06 × 10³ | 0.001 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Sgr), pyruvate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.18 | 10 | 0.018 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | 2-hydroxy-2-methyl-3-ketobutyrate | 3.6 | 9 | 0.4 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | 2-methylacetoacetate | 2 | 40 | 0.05 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), 2-methylacetoacetate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | 3-hydroxy-3-ethyl-2-ketobutyrate | 1.4 | 50 | 0.028 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | 3-hydroxy-3-methyl-2-ketobutyrate | 5.2 | 8.6 | 0.53 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | hydroxypyruvate | 2 | 60 | 0.03 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), hydroxypyruvate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | pyrroline-5-carboxylate | — | — | 0.0028 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase (KARI, Spr) | Streptomyces pristinaespiralis | pyruvate | 3 | 1.08 × 10³ | 0.003 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase (KARI, Spr), pyruvate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | 2-hydroxy-2-ethyl-3-ketobutyrate | 3.2 | 67 | 0.047 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | 2-hydroxy-2-methyl-3-ketobutyrate | 1.1 | 1.6 | 0.687 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | 2-methylacetoacetate | 1 | 50 | 0.02 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), 2-methylacetoacetate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | 3-hydroxy-3-ethyl-2-ketobutyrate | 1.8 | 45 | 0.04 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | 3-hydroxy-3-methyl-2-ketobutyrate | 2.2 | 3 | 0.733 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | pyrroline-5-carboxylate | — | — | 0.0024 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase KARI1 (Sco1) | Streptomyces coelicolor | pyruvate | 2.8 | 950 | 0.003 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sco1), pyruvate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | 2-hydroxy-2-ethyl-3-ketobutyrate | 3.6 | 71 | 0.05 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | 2-hydroxy-2-methyl-3-ketobutyrate | 0.7 | 1.2 | 0.583 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | 2-methylacetoacetate | 0.675 | 45 | 0.015 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), 2-methylacetoacetate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | 3-hydroxy-3-ethyl-2-ketobutyrate | 1.3 | 50 | 0.046 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | 3-hydroxy-3-methyl-2-ketobutyrate | 2.8 | 4 | 0.7 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | pyrroline-5-carboxylate | — | — | 0.0037 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase KARI1 (Sli1) | Streptomyces lividans | pyruvate | 6.7 | 1.12 × 10³ | 0.006 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI1 (Sli1), pyruvate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.39 | 12 | 0.032 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | 2-hydroxy-2-methyl-3-ketobutyrate | 0.24 | 12 | 0.02 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | 2-methylacetoacetate | 0.6 | 60 | 0.01 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), 2-methylacetoacetate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | 3-hydroxy-3-ethyl-2-ketobutyrate | 0.8 | 20 | 0.04 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | 3-hydroxy-3-methyl-2-ketobutyrate | 0.3 | 10 | 0.03 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | hydroxypyruvate | 0.5 | 40 | 0.06 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), hydroxypyruvate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | pyrroline-5-carboxylate | — | — | 0.003 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase KARI2 (Sco2) | Streptomyces coelicolor | pyruvate | 5.6 | 700 | 0.008 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sco2), pyruvate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.12 | 5 | 0.048 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | 2-hydroxy-2-methyl-3-ketobutyrate | 0.28 | 9.3 | 0.03 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | 2-methylacetoacetate | 0.5 | 50 | 0.01 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), 2-methylacetoacetate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | 3-hydroxy-3-ethyl-2-ketobutyrate | 0.9 | 20 | 0.046 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | 3-hydroxy-3-methyl-2-ketobutyrate | 0.4 | 8.6 | 0.045 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | hydroxypyruvate | 0.7 | 50 | 0.04 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), hydroxypyruvate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | pyrroline-5-carboxylate | — | — | 0.0061 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase KARI2 (Sli2) | Streptomyces lividans | pyruvate | 3 | 1.01 × 10³ | 0.003 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Sli2), pyruvate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | 2-hydroxy-2-ethyl-3-ketobutyrate | 0.3 | 25 | 0.012 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), 2-hydroxy-2-ethyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | 2-hydroxy-2-methyl-3-ketobutyrate | 2 | 1.8 | 1.111 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), 2-hydroxy-2-methyl-3-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | 2-methylacetoacetate | 2 | 50 | 0.04 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), 2-methylacetoacetate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | 3-hydroxy-3-ethyl-2-ketobutyrate | 0.5 | 50 | 0.01 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), 3-hydroxy-3-ethyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | 3-hydroxy-3-methyl-2-ketobutyrate | 2.5 | 5 | 0.5 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), 3-hydroxy-3-methyl-2-ketobutyrate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | hydroxypyruvate | 2 | 50 | 0.05 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), hydroxypyruvate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | pyrroline-5-carboxylate | — | — | 0.0038 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), pyrroline-5-carboxylate |
| ketol-acid reductoisomerase KARI2 (Svi2) | Streptomyces viridifaciens | pyruvate | 5.7 | 1.14 × 10³ | 0.005 | pH 8, 25 °C | Open record: ketol-acid reductoisomerase KARI2 (Svi2), pyruvate |
| pyrroline-5-carboxylate reductase (P5CR) | Streptomyces coelicolor | pyrroline-5-carboxylate | — | — | 0.601 | pH 8, 25 °C | Open record: pyrroline-5-carboxylate reductase (P5CR), pyrroline-5-carboxylate |