Values CatLog read from this paper

Reversal of the nucleotide specificity of ketol acid reductoisomerase by site-directed mutagenesis identifies the NADPH binding site

PMID 9015391doi:10.1006/abbi.1996.9802Open in Browse

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CatLog read 17 records (51 values) from this paper: ketol acid reductoisomerase (KARI) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.86

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
ketol acid reductoisomerase (KARI)	Escherichia coli	NADH	0.0018	0.206	0.00873	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI), NADH
ketol acid reductoisomerase (KARI)	Escherichia coli	NADP	0.052	0.0042	12.3	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI), NADP
ketol acid reductoisomerase (KARI)	Escherichia coli	NADPH	0.12	0.0073	16.5	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI), NADPH
ketol acid reductoisomerase (KARI)variant K69L	Escherichia coli	NADP	3.2 × 10⁻⁴	0.0089	0.035	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant K69L, NADP
ketol acid reductoisomerase (KARI)variant K69L	Escherichia coli	NADPH	0.212	0.0076	28	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant K69L, NADPH
ketol acid reductoisomerase (KARI)variant K75Q	Escherichia coli	NADP	6.7 × 10⁻⁴	0.0112	0.06	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant K75Q, NADP
ketol acid reductoisomerase (KARI)variant K75Q	Escherichia coli	NADPH	0.0093	0.0025	3.8	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant K75Q, NADPH
ketol acid reductoisomerase (KARI)variant R68D/K69L/K75V/R76D	Escherichia coli	NADH	0.088	0.019	4.5	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R68D/K69L/K75V/R76D, NADH
ketol acid reductoisomerase (KARI)variant R68D/K69L/K75V/R76D	Escherichia coli	NADPH	0.0328	0.222	0.148	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R68D/K69L/K75V/R76D, NADPH
ketol acid reductoisomerase (KARI)variant R68Q	Escherichia coli	NADP	1.5 × 10⁻⁴	0.029	0.0052	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R68Q, NADP
ketol acid reductoisomerase (KARI)variant R68Q	Escherichia coli	NADPH	0.0067	0.0245	0.27	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R68Q, NADPH
ketol acid reductoisomerase (KARI)variant R76D	Escherichia coli	NADH	0.088	0.082	1.08	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R76D, NADH
ketol acid reductoisomerase (KARI)variant R76D	Escherichia coli	NADPH	0.03	0.401	0.075	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R76D, NADPH
ketol acid reductoisomerase (KARI)variant R76Q	Escherichia coli	NADP	2 × 10⁻⁴	0.072	0.0028	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R76Q, NADP
ketol acid reductoisomerase (KARI)variant R76Q	Escherichia coli	NADPH	0.0062	0.0365	0.17	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R76Q, NADPH
ketol acid reductoisomerase (KARI)variant R76Q/R68A	Escherichia coli	NADH	0.037	0.193	18	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R76Q/R68A, NADH
ketol acid reductoisomerase (KARI)variant R76Q/R68A	Escherichia coli	NADPH	0.0043	0.16	0.027	pH 8, 22 °C	Open record: ketol acid reductoisomerase (KARI) variant R76Q/R68A, NADPH