Values CatLog read from this paper

Engineered ketol-acid reductoisomerase and alcohol dehydrogenase enable anaerobic 2-methylpropan-1-ol production at theoretical yield in Escherichia coli

PMID 21515217doi:10.1016/j.ymben.2011.02.004Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 18 records (54 values) from this paper: ketol-acid reductoisomerase (KARI, IlvC), alcohol dehydrogenase (AdhA) from Escherichia coli, Lactococcus lactis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.2, EC 1.1.1.86

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
alcohol dehydrogenase (AdhA)	Lactococcus lactis	isobutyraldehyde	30	11.7	2.8	pH 7	Open record: alcohol dehydrogenase (AdhA), isobutyraldehyde
alcohol dehydrogenase (AdhA)variant N219Y/L264V	Lactococcus lactis	isobutyraldehyde	60	2.7	21	pH 7	Open record: alcohol dehydrogenase (AdhA) variant N219Y/L264V, isobutyraldehyde
alcohol dehydrogenase (AdhA)variant Y50F/I212T	Lactococcus lactis	isobutyraldehyde	80	3.9	20	pH 7	Open record: alcohol dehydrogenase (AdhA) variant Y50F/I212T, isobutyraldehyde
alcohol dehydrogenase (AdhA)variant Y50F/I212T/L264V	Lactococcus lactis	isobutyraldehyde	140	1.7	82	pH 7	Open record: alcohol dehydrogenase (AdhA) variant Y50F/I212T/L264V, isobutyraldehyde
ketol-acid reductoisomerase (KARI, IlvC)	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	0.3	1.08	0.3	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC), nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	3.6	0.04	87.3	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC), nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110A	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	2.6	0.04	60.3	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110A, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110A	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	0.6	0.57	1.1	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110A, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	2.3	0.03	74	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110V, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant A71S/R76D/S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	0.2	0.65	0.4	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant A71S/R76D/S78D/Q110V, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110A	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	3.1	0.28	11	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110A, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110A	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	7.2	0.024	302	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110A, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110V	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	3.3	0.14	24.8	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110V, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant Q110V	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	7.2	0.013	569	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant Q110V, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	3.6	0.13	27.6	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	2.5	0.66	3.8	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D, nicotinamide adenine dinucleotide phosphate (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide (reduced)	2	0.065	31.8	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D/Q110V, nicotinamide adenine dinucleotide (reduced)
ketol-acid reductoisomerase (KARI, IlvC)variant S78D/Q110V	Escherichia coli	nicotinamide adenine dinucleotide phosphate (reduced)	2.2	0.065	34.2	pH 7	Open record: ketol-acid reductoisomerase (KARI, IlvC) variant S78D/Q110V, nicotinamide adenine dinucleotide phosphate (reduced)