Values CatLog read from this paper
Kinetic and crystallographic analysis of active site mutants of Escherichia coli gamma-aminobutyrate aminotransferase
PMID 15723541doi:10.1021/bi048657aOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 53 records (69 values) from this paper: γ-aminobutyrate aminotransferase (GABA-AT) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | 2-oxoglutarateping-pong GABA/α-KG transamination; global fit | — | 1.07 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | 2-oxoglutarateL-aspartate/α-KG transamination; cosubstrate L-aspartate | — | 0.8 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | D,L-α-methylaspartate | 8.6 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | D,L-α-methylglutamate | 9.2 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | D-glutamate | 1.5 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | gamma-aminobutyrate | 47.4 | 5.8 | 8.172 | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | L-aspartate | 0.016 | 150 | 1.1 × 10⁻⁴ calc. | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), L-aspartate |
| γ-aminobutyrate aminotransferase (GABA-AT) | Escherichia coli | L-glutamate | 6 × 10⁻⁵ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT), L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | 2-oxoglutarateping-pong GABA/α-KG transamination; global fit | — | 0.0021 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | 2-oxoglutarateL-aspartate/α-KG transamination; cosubstrate L-aspartate | — | 0.0018 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | D,L-α-methylglutamate | 2.4 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | D-glutamate | 1.8 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | gamma-aminobutyrate | 0.56 | 86 | 0.0065 | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | L-aspartate | 0.073 | 56 | 0.0013 calc. | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, L-aspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S | Escherichia coli | L-glutamate | 1.6 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G | Escherichia coli | D,L-α-methylglutamate | 1.1 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G, D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G | Escherichia coli | gamma-aminobutyrate | 0.003 | — | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G | Escherichia coli | L-glutamate | 9 × 10⁻⁵ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77K | Escherichia coli | 2-oxoglutarate | — | 0.72 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77K, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77K | Escherichia coli | D,L-α-methylaspartate | 3.8 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77K, D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77K | Escherichia coli | D,L-α-methylglutamate | 0.00104 | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77K, D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77K | Escherichia coli | D-glutamate | 6.9 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77K, D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77K | Escherichia coli | gamma-aminobutyrate | 0.25 | 5.9 | 0.042 | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77K, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77K | Escherichia coli | L-glutamate | 5.2 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77K, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77R | Escherichia coli | D,L-α-methylaspartate | 1.4 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77R, D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77R | Escherichia coli | D-glutamate | 1.1 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77R, D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77R | Escherichia coli | gamma-aminobutyrate | 5 × 10⁻⁴ | — | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77R, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50G/C77R | Escherichia coli | L-glutamate | 1.9 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50G/C77R, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80D | Escherichia coli | D,L-α-methylaspartate | 7.4 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80D, D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80D | Escherichia coli | D,L-α-methylglutamate | 0.0017 | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80D, D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80D | Escherichia coli | D-glutamate | 9.8 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80D, D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80D | Escherichia coli | gamma-aminobutyrate | 2.3 × 10⁻⁴ | — | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80D, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80D | Escherichia coli | L-glutamate | 6.1 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80D, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80T | Escherichia coli | 2-oxoglutarate | — | 0.063 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80T, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80T | Escherichia coli | D,L-α-methylaspartate | 1.4 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80T, D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80T | Escherichia coli | D-glutamate | 1.5 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80T, D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50H/V80T | Escherichia coli | gamma-aminobutyrate | 0.106 | 146 | 7.3 × 10⁻⁴ | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50H/V80T, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50N/V80D | Escherichia coli | gamma-aminobutyrate | 0.0075 | — | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50N/V80D, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50N/V80T | Escherichia coli | D,L-α-methylaspartate | 1.4 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50N/V80T, D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50N/V80T | Escherichia coli | D,L-α-methylglutamate | 2.8 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50N/V80T, D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50N/V80T | Escherichia coli | gamma-aminobutyrate | 0.0058 | — | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50N/V80T, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50N/V80T | Escherichia coli | L-glutamate | 1.9 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50N/V80T, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant E211S/I50Q/G295Y/V241A | Escherichia coli | gamma-aminobutyrate | 0.004 | — | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant E211S/I50Q/G295Y/V241A, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q | Escherichia coli | 2-oxoglutarate | — | 46 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q | Escherichia coli | D,L-α-methylaspartate | 4 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q, D,L-α-methylaspartate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q | Escherichia coli | D,L-α-methylglutamate | 7 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q, D,L-α-methylglutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q | Escherichia coli | D-glutamate | 1 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q, D-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q | Escherichia coli | gamma-aminobutyrate | 13.5 | 68 | 0.199 | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q | Escherichia coli | L-glutamate | 2.2 × 10⁻⁴ | — | — | 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q, L-glutamate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q/G295Y | Escherichia coli | 2-oxoglutarate | — | 0.78 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q/G295Y, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant I50Q/G295Y | Escherichia coli | gamma-aminobutyrate | 0.028 | 4.7 | 0.0059 | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant I50Q/G295Y, gamma-aminobutyrate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant V241A | Escherichia coli | 2-oxoglutarate | — | 0.13 | — | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant V241A, 2-oxoglutarate |
| γ-aminobutyrate aminotransferase (GABA-AT)variant V241A | Escherichia coli | gamma-aminobutyrate | 1.93 | 30 | 0.064 | pH 7.8, 25 °C | Open record: γ-aminobutyrate aminotransferase (GABA-AT) variant V241A, gamma-aminobutyrate |