CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutation of active site residues of insulin-degrading enzyme alters allosteric interactions

PMID 15749695doi:10.1074/jbc.m501896200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 7 records (13 values) from this paper: insulin-degrading enzyme (IDE) from Rattus norvegicus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.4.24.56

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
insulin-degrading enzyme (IDE)Rattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine1.626 × 10⁵——pH 7.3Open
insulin-degrading enzyme (IDE)variant E111ARattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine17.50.20485.8 calc.pH 7.3Open
insulin-degrading enzyme (IDE)variant E111FRattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine11.40.0274416 calc.pH 7.3Open
insulin-degrading enzyme (IDE)variant E111LRattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine6.50.014460 calc.pH 7.3Open
insulin-degrading enzyme (IDE)variant E111VRattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine2.40.0156150 calc.pH 7.3Open
insulin-degrading enzyme (IDE)variant H112DRattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine23.70.0832285 calc.pH 7.3Open
insulin-degrading enzyme (IDE)variant H112QRattus norvegicus2-aminobenzoyl-GGFLRKHGQ-N-(2,4-dinitrophenyl)ethylenediamine19530.15821.235 × 10⁴ calc.pH 7.3Open