Values CatLog read from this paper
Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida
PMID 15930043doi:10.1093/protein/gzi035Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 37 records (95 values) from this paper: pyruvate decarboxylase, benzoylformate decarboxylase from Zymomonas mobilis, Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxobutanoic acid | 17.3 | — | — | pH 6, 30 °C | Open record: benzoylformate decarboxylase, 2-oxobutanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxohexanoic acid | 21 | 4.1 | 5.1 | pH 6, 30 °C | Open record: benzoylformate decarboxylase, 2-oxohexanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxopentanoic acid | 44.3 | 6 | 7.4 | pH 6, 30 °C | Open record: benzoylformate decarboxylase, 2-oxopentanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 4-methyl-2-oxohexanoic acid | 8.6 | — | — | pH 6, 30 °C | Open record: benzoylformate decarboxylase, 4-methyl-2-oxohexanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 4-methyl-2-oxopentanoic acid | 11 | 9.6 | 1.1 | pH 6, 30 °C | Open record: benzoylformate decarboxylase, 4-methyl-2-oxopentanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 1349 | 0.8 | 1686 | pH 6, 30 °C | Open record: benzoylformate decarboxylase, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylase | Pseudomonas putida | pyruvic acid | 0.1 | — | — | pH 6, 30 °C | Open record: benzoylformate decarboxylase, pyruvic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 2-oxobutanoic acid | 18.8 | 18.3 | 1 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, 2-oxobutanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 2-oxohexanoic acid | 71.8 | 1 | 71.8 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, 2-oxohexanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 2-oxopentanoic acid | 40.6 | 1.2 | 33.8 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, 2-oxopentanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 4-methyl-2-oxohexanoic acid | 9.8 | 1.1 | 8.9 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, 4-methyl-2-oxohexanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 4-methyl-2-oxopentanoic acid | 38 | 3.4 | 11 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, 4-methyl-2-oxopentanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 62 | 0.2 | 310 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | pyruvic acid | 5.6 | — | — | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I, pyruvic acid |
| benzoylformate decarboxylasevariant A460I/F464I | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 21 | 1.1 | 19 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant A460I/F464I, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylasevariant F464I | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 123 | 2.3 | 53 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant F464I, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| pyruvate decarboxylase | Zymomonas mobilis | 2-oxobutanoic acid | 320 | 4.7 | 68 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase, 2-oxobutanoic acid |
| pyruvate decarboxylase | Zymomonas mobilis | 2-oxohexanoic acid | 4 | 12.7 | 0.3 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase, 2-oxohexanoic acid |
| pyruvate decarboxylase | Zymomonas mobilis | 2-oxopentanoic acid | 53 | 7.6 | 7 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase, 2-oxopentanoic acid |
| pyruvate decarboxylase | Zymomonas mobilis | 4-methyl-2-oxohexanoic acid | 0.4 | — | — | pH 6.5, 30 °C | Open record: pyruvate decarboxylase, 4-methyl-2-oxohexanoic acid |
| pyruvate decarboxylase | Zymomonas mobilis | pyruvic acid | 486 | 1.1 | 442 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase, pyruvic acid |
| pyruvate decarboxylasevariant I472A | Zymomonas mobilis | 2-oxobutanoic acid | 250 | 6.7 | 37 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A, 2-oxobutanoic acid |
| pyruvate decarboxylasevariant I472A | Zymomonas mobilis | 2-oxohexanoic acid | 130 | 0.2 | 650 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A, 2-oxohexanoic acid |
| pyruvate decarboxylasevariant I472A | Zymomonas mobilis | 2-oxopentanoic acid | 220 | 2.5 | 87 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A, 2-oxopentanoic acid |
| pyruvate decarboxylasevariant I472A | Zymomonas mobilis | 4-methyl-2-oxohexanoic acid | 7.7 | 1.7 | 4.5 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A, 4-methyl-2-oxohexanoic acid |
| pyruvate decarboxylasevariant I472A | Zymomonas mobilis | benzoylformic acid (2-oxo-2-phenylacetic acid) | 6.9 | 1.8 | 3.8 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| pyruvate decarboxylasevariant I472A | Zymomonas mobilis | pyruvic acid | 200 | 7.8 | 26 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A, pyruvic acid |
| pyruvate decarboxylasevariant I472A/I476F | Zymomonas mobilis | 2-oxobutanoic acid | 9 | 50 | 0.2 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A/I476F, 2-oxobutanoic acid |
| pyruvate decarboxylasevariant I472A/I476F | Zymomonas mobilis | 2-oxohexanoic acid | 82 | 0.5 | 164 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A/I476F, 2-oxohexanoic acid |
| pyruvate decarboxylasevariant I472A/I476F | Zymomonas mobilis | 2-oxopentanoic acid | 30 | 11 | 2.7 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A/I476F, 2-oxopentanoic acid |
| pyruvate decarboxylasevariant I472A/I476F | Zymomonas mobilis | 4-methyl-2-oxohexanoic acid | 8.1 | 3.7 | 2.2 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A/I476F, 4-methyl-2-oxohexanoic acid |
| pyruvate decarboxylasevariant I472A/I476F | Zymomonas mobilis | benzoylformic acid (2-oxo-2-phenylacetic acid) | 1.2 | 4.4 | 0.3 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A/I476F, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| pyruvate decarboxylasevariant I472A/I476F | Zymomonas mobilis | pyruvic acid | 8 | 50 | 0.2 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I472A/I476F, pyruvic acid |
| pyruvate decarboxylasevariant I476F | Zymomonas mobilis | 2-oxobutanoic acid | 32 | — | — | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I476F, 2-oxobutanoic acid |
| pyruvate decarboxylasevariant I476F | Zymomonas mobilis | 2-oxohexanoic acid | 0.8 | — | — | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I476F, 2-oxohexanoic acid |
| pyruvate decarboxylasevariant I476F | Zymomonas mobilis | 2-oxopentanoic acid | 11 | — | — | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I476F, 2-oxopentanoic acid |
| pyruvate decarboxylasevariant I476F | Zymomonas mobilis | pyruvic acid | 77 | 2.6 | 30 | pH 6.5, 30 °C | Open record: pyruvate decarboxylase variant I476F, pyruvic acid |