EC 4.1.1.7: benzoylformate decarboxylase
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CatLog read 74 records for EC 4.1.1.7 from 6 papers, for 1 organism. Names in these papers: benzoylformate decarboxylase, benzoylformate decarboxylase (BFDC).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 100 records for EC 4.1.1.7 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.1.7 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Engineering the substrate binding site of benzoylformate decarboxylase | Pseudomonas putida | 20 |
| Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism | Pseudomonas putida | 17 |
| Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida | Pseudomonas putida | 16 |
| doi:10.3390/catal6120190 | Pseudomonas putida | 11 |
| Kinetics and mechanism of benzoylformate decarboxylase using 13C and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues | Pseudomonas putida | 6 |
| Structural and kinetic analysis of catalysis by a thiamin diphosphate-dependent enzyme, benzoylformate decarboxylase | Pseudomonas putida | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| benzoylformate decarboxylase | Pseudomonas putida | 2-(3-fluorophenyl)-2-oxoacetate | — | 0.023 | — | pH 7.2, 25 °C | PMID 3378056 | Open record: benzoylformate decarboxylase, 2-(3-fluorophenyl)-2-oxoacetate |
| benzoylformate decarboxylase | Pseudomonas putida | 2-(4-chlorophenyl)-2-oxoacetate | — | 0.18 | — | pH 7.2, 25 °C | PMID 3378056 | Open record: benzoylformate decarboxylase, 2-(4-chlorophenyl)-2-oxoacetate |
| benzoylformate decarboxylase | Pseudomonas putida | 2-(4-methoxyphenyl)-2-oxoacetate | — | 0.056 | — | pH 7.2, 25 °C | PMID 3378056 | Open record: benzoylformate decarboxylase, 2-(4-methoxyphenyl)-2-oxoacetate |
| benzoylformate decarboxylase | Pseudomonas putida | 2-(4-methylphenyl)-2-oxoacetate | — | 0.19 | — | pH 7.2, 25 °C | PMID 3378056 | Open record: benzoylformate decarboxylase, 2-(4-methylphenyl)-2-oxoacetate |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxo-2-phenylacetate | — | 0.059 | — | pH 7.2, 25 °C | PMID 3378056 | Open record: benzoylformate decarboxylase, 2-oxo-2-phenylacetate |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxo-2-phenylacetate | — | 0.08 | — | pH 6.1, 25 °C | PMID 3378056 | Open record: benzoylformate decarboxylase, 2-oxo-2-phenylacetate |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxobutanoic acid | 17.3 | — | — | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, 2-oxobutanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxohexanoic acid | 21 | 4.1 | 5.1 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, 2-oxohexanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 2-oxopentanoic acid | 44.3 | 6 | 7.4 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, 2-oxopentanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 4-methyl-2-oxohexanoic acid | 8.6 | — | — | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, 4-methyl-2-oxohexanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | 4-methyl-2-oxopentanoic acid | 11 | 9.6 | 1.1 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, 4-methyl-2-oxopentanoic acid |
| benzoylformate decarboxylase | Pseudomonas putida | benzoylformate | 241 | 0.37 | 651 | pH 7, 20 °C | PMID 12590569 | Open record: benzoylformate decarboxylase, benzoylformate |
| benzoylformate decarboxylase | Pseudomonas putida | benzoylformate | 320 | 0.27 | 1.18 × 10³ | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase, benzoylformate |
| benzoylformate decarboxylase | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 1349 | 0.8 | 1686 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylase | Pseudomonas putida | pyruvic acid | 0.1 | — | — | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase, pyruvic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 2-oxobutanoic acid | 18.8 | 18.3 | 1 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, 2-oxobutanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 2-oxohexanoic acid | 71.8 | 1 | 71.8 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, 2-oxohexanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 2-oxopentanoic acid | 40.6 | 1.2 | 33.8 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, 2-oxopentanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 4-methyl-2-oxohexanoic acid | 9.8 | 1.1 | 8.9 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, 4-methyl-2-oxohexanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | 4-methyl-2-oxopentanoic acid | 38 | 3.4 | 11 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, 4-methyl-2-oxopentanoic acid |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 62 | 0.2 | 310 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylasevariant A460I | Pseudomonas putida | pyruvic acid | 5.6 | — | — | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I, pyruvic acid |
| benzoylformate decarboxylasevariant A460I/F464I | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 21 | 1.1 | 19 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant A460I/F464I, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylasevariant F464I | Pseudomonas putida | benzoylformic acid (2-oxo-2-phenylacetic acid) | 123 | 2.3 | 53 | pH 6, 30 °C | PMID 15930043 | Open record: benzoylformate decarboxylase variant F464I, benzoylformic acid (2-oxo-2-phenylacetic acid) |
| benzoylformate decarboxylasevariant H281A | Pseudomonas putida | benzoylformate | 1.41 | 1.68 | 0.84 | pH 7, 20 °C | PMID 12590569 | Open record: benzoylformate decarboxylase variant H281A, benzoylformate |
| benzoylformate decarboxylasevariant H281A | Pseudomonas putida | benzoylformate | 2.1 | 1.2 | 1.7 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281A, benzoylformate |
| benzoylformate decarboxylasevariant H281F | Pseudomonas putida | benzoylformate | 65 | 0.54 | 120 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281F, benzoylformate |
| benzoylformate decarboxylasevariant H281N | Pseudomonas putida | benzoylformate | 19 | 2.9 | 6.4 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281N, benzoylformate |
| benzoylformate decarboxylasevariant H281Q | Pseudomonas putida | benzoylformate | 8.6 | 0.29 | 29 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281Q, benzoylformate |
| benzoylformate decarboxylasevariant H281T | Pseudomonas putida | benzoylformate | 2 | 0.9 | 2.2 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281T, benzoylformate |
| benzoylformate decarboxylasevariant H281W | Pseudomonas putida | benzoylformate | 17 | 0.29 | 57 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281W, benzoylformate |
| benzoylformate decarboxylasevariant H281Y | Pseudomonas putida | benzoylformate | 6.9 | 0.49 | 14 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H281Y, benzoylformate |
| benzoylformate decarboxylasevariant H70A | Pseudomonas putida | benzoylformate | 0.07 | 1.92 | 0.037 | pH 7, 20 °C | PMID 12590569 | Open record: benzoylformate decarboxylase variant H70A, benzoylformate |
| benzoylformate decarboxylasevariant H70A | Pseudomonas putida | benzoylformate | 0.46 | 1.5 | 0.31 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H70A, benzoylformate |
| benzoylformate decarboxylasevariant H70F | Pseudomonas putida | benzoylformate | 4.5 | 0.85 | 5.3 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H70F, benzoylformate |
| benzoylformate decarboxylasevariant H70L | Pseudomonas putida | benzoylformate | 14 | 0.38 | 36 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H70L, benzoylformate |
| benzoylformate decarboxylasevariant H70S | Pseudomonas putida | benzoylformate | 5.6 | 0.94 | 5.9 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H70S, benzoylformate |
| benzoylformate decarboxylasevariant H70T | Pseudomonas putida | benzoylformate | 42 | 0.89 | 47 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant H70T, benzoylformate |
| benzoylformate decarboxylasevariant S26A | Pseudomonas putida | benzoylformate | 4.45 | 8.62 | 0.52 | pH 7, 20 °C | PMID 12590569 | Open record: benzoylformate decarboxylase variant S26A, benzoylformate |
| benzoylformate decarboxylasevariant S26A | Pseudomonas putida | benzoylformate | 15 | 7.8 | 1.9 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant S26A, benzoylformate |
| benzoylformate decarboxylasevariant S26L | Pseudomonas putida | benzoylformate | 132 | 1.2 | 110 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant S26L, benzoylformate |
| benzoylformate decarboxylasevariant S26M | Pseudomonas putida | benzoylformate | 26 | 0.8 | 32 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant S26M, benzoylformate |
| benzoylformate decarboxylasevariant S26T | Pseudomonas putida | benzoylformate | 94 | 0.85 | 110 | pH 6, 30 °C | PMID 18398009 | Open record: benzoylformate decarboxylase variant S26T, benzoylformate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 2-ketobutyrate | 4 | 7.5 | 0.5 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC), 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 2-ketohexanoate | 5.3 | 4.1 | 1.3 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC), 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 2-ketopentanoate | 11 | 6 | 1.8 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC), 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 4-(methylsulfanyl)-2-oxobutanoate | 4.5 | 1.3 | 3.5 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC), 4-(methylsulfanyl)-2-oxobutanoate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | benzoylformate | 320 | 0.27 | 1.18 × 10³ | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC), benzoylformate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | pyruvate | — | — | 0.012 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC), pyruvate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | 2-ketobutyrate | 6 | 1.3 | 4.6 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | 2-ketohexanoate | 4.4 | 0.28 | 16 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | 2-ketopentanoate | 6.2 | 0.28 | 22 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | benzoylformate | 1.7 | 0.04 | 43 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, benzoylformate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | pyruvate | 1.4 | 21 | 0.065 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, pyruvate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | 2-ketobutyrate | 7.5 | 4.3 | 1.7 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L, 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | 2-ketohexanoate | 1.1 | 1.3 | 0.86 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L, 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | 2-ketopentanoate | 3.3 | 1.1 | 3 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L, 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | benzoylformate | 110 | 0.45 | 244 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L, benzoylformate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | pyruvate | 2 | 4.8 | 0.42 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L, pyruvate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-ketobutyrate | 5.8 | 0.42 | 14 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-ketohexanoate | 1.2 | 0.15 | 8 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-ketopentanoate | 0.81 | 0.1 | 8.1 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-oxobutanoate | 6 | 0.39 | 16 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-oxobutanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-oxohexanoate | 5.9 | 0.14 | 42 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-oxohexanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-oxopentanoate | 1.1 | 0.15 | 7.5 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-oxopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 3-methyl-2-oxobutanoate | 12 | 0.77 | 15 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 3-methyl-2-oxobutanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 3-methyl-2-oxopentanoate | 16 | 1.2 | 13 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 3-methyl-2-oxopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 4-(methylsulfanyl)-2-oxobutanoate | 4.6 | 0.14 | 33 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 4-(methylsulfanyl)-2-oxobutanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 4-methyl-2-oxopentanoate | 2.7 | 0.14 | 20 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 4-methyl-2-oxopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | benzoylformate | 25 | 1.5 | 16 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, benzoylformate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | phenylglyoxylate | 15 | 0.45 | 34 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, phenylglyoxylate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | Phenylpyruvate | 2 | 0.82 | 2.5 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, Phenylpyruvate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | pyruvate | 3.6 | 2 | 1.8 | pH 6, 30 °C | PMID 19621900 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, pyruvate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | Pyruvate | 3.3 | 4.6 | 0.72 | pH 6, 30 °C | doi:10.3390/catal6120190 | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, Pyruvate |