Values CatLog read from this paper
Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism
PMID 18398009doi:10.1073/pnas.0709657105Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (51 values) from this paper: benzoylformate decarboxylase from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| benzoylformate decarboxylase | Pseudomonas putida | benzoylformate | 320 | 0.27 | 1.18 × 10³ | pH 6, 30 °C | Open record: benzoylformate decarboxylase, benzoylformate |
| benzoylformate decarboxylasevariant H281A | Pseudomonas putida | benzoylformate | 2.1 | 1.2 | 1.7 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281A, benzoylformate |
| benzoylformate decarboxylasevariant H281F | Pseudomonas putida | benzoylformate | 65 | 0.54 | 120 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281F, benzoylformate |
| benzoylformate decarboxylasevariant H281N | Pseudomonas putida | benzoylformate | 19 | 2.9 | 6.4 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281N, benzoylformate |
| benzoylformate decarboxylasevariant H281Q | Pseudomonas putida | benzoylformate | 8.6 | 0.29 | 29 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281Q, benzoylformate |
| benzoylformate decarboxylasevariant H281T | Pseudomonas putida | benzoylformate | 2 | 0.9 | 2.2 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281T, benzoylformate |
| benzoylformate decarboxylasevariant H281W | Pseudomonas putida | benzoylformate | 17 | 0.29 | 57 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281W, benzoylformate |
| benzoylformate decarboxylasevariant H281Y | Pseudomonas putida | benzoylformate | 6.9 | 0.49 | 14 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H281Y, benzoylformate |
| benzoylformate decarboxylasevariant H70A | Pseudomonas putida | benzoylformate | 0.46 | 1.5 | 0.31 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H70A, benzoylformate |
| benzoylformate decarboxylasevariant H70F | Pseudomonas putida | benzoylformate | 4.5 | 0.85 | 5.3 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H70F, benzoylformate |
| benzoylformate decarboxylasevariant H70L | Pseudomonas putida | benzoylformate | 14 | 0.38 | 36 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H70L, benzoylformate |
| benzoylformate decarboxylasevariant H70S | Pseudomonas putida | benzoylformate | 5.6 | 0.94 | 5.9 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H70S, benzoylformate |
| benzoylformate decarboxylasevariant H70T | Pseudomonas putida | benzoylformate | 42 | 0.89 | 47 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant H70T, benzoylformate |
| benzoylformate decarboxylasevariant S26A | Pseudomonas putida | benzoylformate | 15 | 7.8 | 1.9 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant S26A, benzoylformate |
| benzoylformate decarboxylasevariant S26L | Pseudomonas putida | benzoylformate | 132 | 1.2 | 110 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant S26L, benzoylformate |
| benzoylformate decarboxylasevariant S26M | Pseudomonas putida | benzoylformate | 26 | 0.8 | 32 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant S26M, benzoylformate |
| benzoylformate decarboxylasevariant S26T | Pseudomonas putida | benzoylformate | 94 | 0.85 | 110 | pH 6, 30 °C | Open record: benzoylformate decarboxylase variant S26T, benzoylformate |