Values CatLog read from this paper
Engineering the substrate binding site of benzoylformate decarboxylase
PMID 19621900doi:10.1021/bi9008402Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (58 values) from this paper: benzoylformate decarboxylase (BFDC) from Pseudomonas putida.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 2-ketobutyrate | 4 | 7.5 | 0.5 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC), 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 2-ketohexanoate | 5.3 | 4.1 | 1.3 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC), 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | 2-ketopentanoate | 11 | 6 | 1.8 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC), 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | benzoylformate | 320 | 0.27 | 1.18 × 10³ | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC), benzoylformate |
| benzoylformate decarboxylase (BFDC) | Pseudomonas putida | pyruvate | — | — | 0.012 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC), pyruvate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | 2-ketobutyrate | 6 | 1.3 | 4.6 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | 2-ketohexanoate | 4.4 | 0.28 | 16 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | 2-ketopentanoate | 6.2 | 0.28 | 22 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | benzoylformate | 1.7 | 0.04 | 43 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, benzoylformate |
| benzoylformate decarboxylase (BFDC)variant A460Y | Pseudomonas putida | pyruvate | 1.4 | 21 | 0.065 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant A460Y, pyruvate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | 2-ketobutyrate | 7.5 | 4.3 | 1.7 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L, 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | 2-ketohexanoate | 1.1 | 1.3 | 0.86 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L, 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | 2-ketopentanoate | 3.3 | 1.1 | 3 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L, 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | benzoylformate | 110 | 0.45 | 244 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L, benzoylformate |
| benzoylformate decarboxylase (BFDC)variant T377L | Pseudomonas putida | pyruvate | 2 | 4.8 | 0.42 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L, pyruvate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-ketobutyrate | 5.8 | 0.42 | 14 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-ketobutyrate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-ketohexanoate | 1.2 | 0.15 | 8 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-ketohexanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | 2-ketopentanoate | 0.81 | 0.1 | 8.1 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, 2-ketopentanoate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | benzoylformate | 25 | 1.5 | 16 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, benzoylformate |
| benzoylformate decarboxylase (BFDC)variant T377L/A460Y | Pseudomonas putida | pyruvate | 3.6 | 2 | 1.8 | pH 6, 30 °C | Open record: benzoylformate decarboxylase (BFDC) variant T377L/A460Y, pyruvate |