CatLogenzyme kinetics catalog

Values CatLog read from this paper

Characterization of the functional role of allosteric site residue Asp102 in the regulatory mechanism of human mitochondrial NAD(P)+-dependent malate dehydrogenase (malic enzyme)

PMID 15989682doi:10.1042/bj20050641Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (32 values) from this paper: mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.39

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensL-malateno fumarate130.512.9510.08 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensL-malate3 mM fumarate206.43.5358.5 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensL-malate—4.41—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensNAD+no fumarate—1.03—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensNAD+3 mM fumarate—0.35—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensNAD+—0.22—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensL-malateno fumarate114.79.1212.6 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensL-malate3 mM fumarate127.13.8133.4 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensL-malate—4.53—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensNAD+no fumarate—0.4—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensNAD+3 mM fumarate—0.23—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensNAD+—0.21—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensL-malateno fumarate44.1413.013.393 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensL-malate3 mM fumarate60.614.5313.4 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensL-malate—5.6—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensNAD+no fumarate—0.8—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensNAD+3 mM fumarate—0.33—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensNAD+—0.45—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensL-malateno fumarate99.067.8612.6 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensL-malate3 mM fumarate111.15.4720.3 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensL-malate10 mM fumarate—6.87—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensNAD+no fumarate—0.44—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensNAD+3 mM fumarate—0.31—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensNAD+10 mM fumarate—0.33—pH 7.4, 30 °COpen