CatLogenzyme kinetics catalog

EC 1.1.1.39: malate dehydrogenase (decarboxylating)

OxidoreductasesOpen in Browse

CatLog read 36 records for EC 1.1.1.39 from 2 papers, for 2 organisms. Names in these papers: mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme), malic enzyme (TkME).

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 84 records for EC 1.1.1.39 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.1.1.39 in Browse.

Papers

PaperOrganismsRecords
Characterization of the functional role of allosteric site residue Asp102 in the regulatory mechanism of human mitochondrial NAD(P)+-dependent malate dehydrogenase (malic enzyme)Homo sapiens24
Directed evolution of thermotolerant malic enzyme for improved malate productionThermococcus kodakarensis12

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
malic enzyme (TkME)Thermococcus kodakarensisNAD+4.692.062.27pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)Thermococcus kodakarensisNADP+31.10.0083.76 × 10³pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant I310VThermococcus kodakarensisNAD+37.76.555.76pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant I310VThermococcus kodakarensisNADP+43.40.0212.07 × 10³pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant K228RThermococcus kodakarensisNAD+3.371.282.64pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant K228RThermococcus kodakarensisNADP+2.970.019156pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant R221GThermococcus kodakarensisNAD+12.72.295.57pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant R221GThermococcus kodakarensisNADP+42.60.0251.7 × 10³pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant R221G/K228RThermococcus kodakarensisNAD+7.440.967.72pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant R221G/K228RThermococcus kodakarensisNADP+6.480.24427.5pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant R221G/K228R/I310VThermococcus kodakarensisNAD+21.31.5413.8pH 8, 70 °CPMID 23932397Open
malic enzyme (TkME)variant R221G/K228R/I310VThermococcus kodakarensisNADP+40.10.384104pH 8, 70 °CPMID 23932397Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensL-malateno fumarate130.512.9510.08 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensL-malate3 mM fumarate206.43.5358.5 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensL-malate—4.41—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensNAD+no fumarate—1.03—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensNAD+3 mM fumarate—0.35—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)Homo sapiensNAD+—0.22—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensL-malateno fumarate114.79.1212.6 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensL-malate3 mM fumarate127.13.8133.4 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensL-malate—4.53—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensNAD+no fumarate—0.4—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensNAD+3 mM fumarate—0.23—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102AHomo sapiensNAD+—0.21—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensL-malateno fumarate44.1413.013.393 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensL-malate3 mM fumarate60.614.5313.4 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensL-malate—5.6—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensNAD+no fumarate—0.8—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensNAD+3 mM fumarate—0.33—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102EHomo sapiensNAD+—0.45—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensL-malateno fumarate99.067.8612.6 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensL-malate3 mM fumarate111.15.4720.3 calc.pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensL-malate10 mM fumarate—6.87—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensNAD+no fumarate—0.44—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensNAD+3 mM fumarate—0.31—pH 7.4, 30 °CPMID 15989682Open
mitochondrial NAD(P)+-dependent malate dehydrogenase (m-NAD-MDH, malic enzyme)variant D102KHomo sapiensNAD+10 mM fumarate—0.33—pH 7.4, 30 °CPMID 15989682Open