Values CatLog read from this paper
Directed evolution of thermotolerant malic enzyme for improved malate production
PMID 23932397doi:10.1016/j.jbiosc.2013.07.005Open in Browse
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CatLog read 12 records (36 values) from this paper: malic enzyme (TkME) from Thermococcus kodakarensis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| malic enzyme (TkME) | Thermococcus kodakarensis | NAD+ | 4.69 | 2.06 | 2.27 | pH 8, 70 °C | Open record: malic enzyme (TkME), NAD+ |
| malic enzyme (TkME) | Thermococcus kodakarensis | NADP+ | 31.1 | 0.008 | 3.76 × 10³ | pH 8, 70 °C | Open record: malic enzyme (TkME), NADP+ |
| malic enzyme (TkME)variant I310V | Thermococcus kodakarensis | NAD+ | 37.7 | 6.55 | 5.76 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant I310V, NAD+ |
| malic enzyme (TkME)variant I310V | Thermococcus kodakarensis | NADP+ | 43.4 | 0.021 | 2.07 × 10³ | pH 8, 70 °C | Open record: malic enzyme (TkME) variant I310V, NADP+ |
| malic enzyme (TkME)variant K228R | Thermococcus kodakarensis | NAD+ | 3.37 | 1.28 | 2.64 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant K228R, NAD+ |
| malic enzyme (TkME)variant K228R | Thermococcus kodakarensis | NADP+ | 2.97 | 0.019 | 156 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant K228R, NADP+ |
| malic enzyme (TkME)variant R221G | Thermococcus kodakarensis | NAD+ | 12.7 | 2.29 | 5.57 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant R221G, NAD+ |
| malic enzyme (TkME)variant R221G | Thermococcus kodakarensis | NADP+ | 42.6 | 0.025 | 1.7 × 10³ | pH 8, 70 °C | Open record: malic enzyme (TkME) variant R221G, NADP+ |
| malic enzyme (TkME)variant R221G/K228R | Thermococcus kodakarensis | NAD+ | 7.44 | 0.96 | 7.72 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant R221G/K228R, NAD+ |
| malic enzyme (TkME)variant R221G/K228R | Thermococcus kodakarensis | NADP+ | 6.48 | 0.244 | 27.5 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant R221G/K228R, NADP+ |
| malic enzyme (TkME)variant R221G/K228R/I310V | Thermococcus kodakarensis | NAD+ | 21.3 | 1.54 | 13.8 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant R221G/K228R/I310V, NAD+ |
| malic enzyme (TkME)variant R221G/K228R/I310V | Thermococcus kodakarensis | NADP+ | 40.1 | 0.384 | 104 | pH 8, 70 °C | Open record: malic enzyme (TkME) variant R221G/K228R/I310V, NADP+ |