Values CatLog read from this paper
A modified consensus approach to mutagenesis inverts the cofactor specificity of Bacillus stearothermophilus lactate dehydrogenase
PMID 16012175doi:10.1093/protein/gzi043Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (27 values) from this paper: L-lactate dehydrogenase (BsLDH) from Bacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| L-lactate dehydrogenase (BsLDH) | Bacillus stearothermophilus | NAD+5 mM FBP (allosteric activator) | 32 | 0.105 | 300 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH), NAD+ |
| L-lactate dehydrogenase (BsLDH) | Bacillus stearothermophilus | NAD+no FBP | 3.6 | 0.167 | 20 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant C81S (S81) | Bacillus stearothermophilus | NAD+5 mM FBP (allosteric activator) | 39 | 0.088 | 430 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant C81S (S81), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant C81S (S81) | Bacillus stearothermophilus | NAD+no FBP | 3.9 | 0.248 | 15 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant C81S (S81), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant F16Q (Q16) | Bacillus stearothermophilus | NAD+5 mM FBP (allosteric activator) | 38 | 0.065 | 590 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant F16Q (Q16), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant F16Q (Q16) | Bacillus stearothermophilus | NAD+no FBP | 4.2 | 0.142 | 30 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant F16Q (Q16), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant F16Q/C81S/N85R (Mut31) | Bacillus stearothermophilus | NAD+5 mM FBP (allosteric activator) | 23 | 0.0081 | 2.8 × 10³ | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant F16Q/C81S/N85R (Mut31), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant F16Q/C81S/N85R (Mut31) | Bacillus stearothermophilus | NAD+no FBP | 13 | 0.042 | 310 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant F16Q/C81S/N85R (Mut31), NAD+ |
| L-lactate dehydrogenase (BsLDH)variant F16Q/I37K/D38S/C81S/N85R (Mut31 I37K:D38S) | Bacillus stearothermophilus | NADP+ | 23 | 4.7 | 5 | pH 8, 25 °C | Open record: L-lactate dehydrogenase (BsLDH) variant F16Q/I37K/D38S/C81S/N85R (Mut31 I37K:D38S), NADP+ |