Values CatLog read from this paper
Identification of a novel trifunctional homoisocitrate dehydrogenase and modulation of the broad substrate specificity through site-directed mutagenesis
PMID 16139794doi:10.1016/j.bbrc.2005.08.139Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (23 values) from this paper: homoisocitrate dehydrogenase (Dra-HICDH) from Deinococcus radiodurans.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| homoisocitrate dehydrogenase (Dra-HICDH) | Deinococcus radiodurans | (2R,3S)-homoisocitrate | 46.2 | 0.211 | 219 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH), (2R,3S)-homoisocitrate |
| homoisocitrate dehydrogenase (Dra-HICDH) | Deinococcus radiodurans | D,L-3-isopropylmalate | 0.371 | 1.33 | 0.279 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH), D,L-3-isopropylmalate |
| homoisocitrate dehydrogenase (Dra-HICDH) | Deinococcus radiodurans | D,L-isocitrate | 42.8 | 0.291 | 147 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH), D,L-isocitrate |
| homoisocitrate dehydrogenase (Dra-HICDH) | Deinococcus radiodurans | NAD+ | 30.3 | 0.193 | 157 calc. | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH), NAD+ |
| homoisocitrate dehydrogenase (Dra-HICDH)variant R87T | Deinococcus radiodurans | (2R,3S)-homoisocitrate | 27.8 | 1.5 | 18.5 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH) variant R87T, (2R,3S)-homoisocitrate |
| homoisocitrate dehydrogenase (Dra-HICDH)variant R87V | Deinococcus radiodurans | (2R,3S)-homoisocitrate | 20.8 | 1 | 20.8 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH) variant R87V, (2R,3S)-homoisocitrate |
| homoisocitrate dehydrogenase (Dra-HICDH)ΔA80 (in-frame deletion of Ala80) | Deinococcus radiodurans | (2R,3S)-homoisocitrate | 5.46 | 0.819 | 6.67 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH) ΔA80 (in-frame deletion of Ala80), (2R,3S)-homoisocitrate |
| homoisocitrate dehydrogenase (Dra-HICDH)ΔA80 (in-frame deletion of Ala80) | Deinococcus radiodurans | D,L-isocitrate | 10.9 | 0.465 | 23.4 | pH 7.8, 30 °C | Open record: homoisocitrate dehydrogenase (Dra-HICDH) ΔA80 (in-frame deletion of Ala80), D,L-isocitrate |