Values CatLog read from this paper
Substrate ambiguity and crystal structure of Pyrococcus furiosus 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase: an ancestral 3-deoxyald-2-ulosonate-phosphate synthase?
PMID 16142893doi:10.1021/bi050577zOpen in Browse
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CatLog read 10 records (20 values) from this paper: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) from Pyrococcus furiosus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | 2-deoxy-D-erythrose 4-phosphate | 3 | 0.006 | 490 | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), 2-deoxy-D-erythrose 4-phosphate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | 2-deoxy-D-ribose 5-phosphate | 1.7 | 2.5 | 0.69 | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), 2-deoxy-D-ribose 5-phosphate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | D-arabinose 5-phosphate | 1.1 | 2.7 | 0.4 | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), D-arabinose 5-phosphate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | D-erythrose 4-phosphate | 1.4 | 0.009 | 160 | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), D-erythrose 4-phosphate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | D-ribose 5-phosphate | 2.5 | 1.58 | 1.6 | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), D-ribose 5-phosphate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | phosphoenolpyruvate20-600 µM PEP varied with 480 µM E4P; cosubstrate E4P | — | 0.093 | — | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), phosphoenolpyruvate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | phosphoenolpyruvate20-600 µM PEP varied with 66 µM 2dE4P; cosubstrate 2dE4P | — | 0.033 | — | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), phosphoenolpyruvate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | phosphoenolpyruvate20-600 µM PEP varied with 9 mM A5P; cosubstrate A5P | — | 0.062 | — | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), phosphoenolpyruvate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | phosphoenolpyruvate20-600 µM PEP varied with 4.5 mM R5P; cosubstrate R5P | — | 0.036 | — | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), phosphoenolpyruvate |
| 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS) | Pyrococcus furiosus | phosphoenolpyruvate28-280 µM PEP varied with 4.5 mM 2dR5P; cosubstrate 2dR5P | — | 0.035 | — | pH 6.8, 60 °C | Open record: 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (Pf-DAH7PS), phosphoenolpyruvate |