CatLogenzyme kinetics catalog

Values CatLog read from this paper

Functional roles of ATP-binding residues in the catalytic site of human mitochondrial NAD(P)+-dependent malic enzyme

PMID 16171388doi:10.1021/bi050510bOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 16 records (24 values) from this paper: mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME) from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 1.1.1.38

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)Homo sapiensL-malateno fumarate added115.58.3913.8 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)Homo sapiensL-malate3 mM fumarate added207.93.0967.3 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)Homo sapiensnicotinamide adenine dinucleotideno fumarate added—0.35—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)Homo sapiensnicotinamide adenine dinucleotide3 mM fumarate added—0.23—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant E314AHomo sapiensL-malateno fumarate added35.143.659.63 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant E314AHomo sapiensL-malate3 mM fumarate added43.922.1920.1 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant E314AHomo sapiensnicotinamide adenine dinucleotideno fumarate added—0.05—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant E314AHomo sapiensnicotinamide adenine dinucleotide3 mM fumarate added—0.03—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant N259AHomo sapiensL-malateno fumarate added78.137.849.97 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant N259AHomo sapiensL-malate3 mM fumarate added157.13.7442.0 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant N259AHomo sapiensnicotinamide adenine dinucleotideno fumarate added—0.42—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant N259AHomo sapiensnicotinamide adenine dinucleotide3 mM fumarate added—0.29—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant R165AHomo sapiensL-malateno fumarate added0.0417.90.0052 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant R165AHomo sapiensL-malate3 mM fumarate added0.0437.810.0055 calc.pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant R165AHomo sapiensnicotinamide adenine dinucleotideno fumarate added—1.67—pH 7.4, 30 °COpen
mitochondrial NAD(P)+-dependent malic enzyme (m-NAD-ME)variant R165AHomo sapiensnicotinamide adenine dinucleotide3 mM fumarate added—0.6—pH 7.4, 30 °COpen