Values CatLog read from this paper
Duck liver 'malic' enzyme. Expression in Escherichia coli and characterization of the wild-type enzyme and site-directed mutants
PMID 1622402doi:10.1042/bj2840869Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (12 values) from this paper: malic enzyme from duck.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| malic enzyme | duck | L-malatepurified from duck liver (natural enzyme) | 160 | 0.073 | 2.2 × 10³ calc. | pH 7, 30 °C | Open record: malic enzyme, L-malate |
| malic enzyme | duck | L-malateexpressed from pUC-8 in E. coli TG-2 | 169 | 0.081 | 2.1 × 10³ calc. | pH 7, 30 °C | Open record: malic enzyme, L-malate |
| malic enzyme | duck | NADP+purified from duck liver (natural enzyme) | — | 0.0046 | — | pH 7, 30 °C | Open record: malic enzyme, NADP+ |
| malic enzyme | duck | NADP+expressed from pUC-8 in E. coli TG-2 | — | 0.005 | — | pH 7, 30 °C | Open record: malic enzyme, NADP+ |
| malic enzymevariant C99S | duck | L-malate | 56 | 0.292 | 190 calc. | pH 7, 30 °C | Open record: malic enzyme variant C99S, L-malate |
| malic enzymevariant C99S | duck | NADP+ | — | 0.0047 | — | pH 7, 30 °C | Open record: malic enzyme variant C99S, NADP+ |
| malic enzymevariant R70Q | duck | L-malate | 113 | 0.126 | 897 calc. | pH 7, 30 °C | Open record: malic enzyme variant R70Q, L-malate |
| malic enzymevariant R70Q | duck | NADP+ | — | 0.0036 | — | pH 7, 30 °C | Open record: malic enzyme variant R70Q, NADP+ |