Values CatLog read from this paper

Kinetic and mutation analyses of aspartate kinase from Thermus flavus

PMID 16232547doi:10.1016/s1389-1723(99)80146-7Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 56 records (84 values) from this paper: aspartate kinase from Thermus flavus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 28 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.2.4

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
aspartate kinase	Thermus flavus	adenosine 5'-triphosphate	—	0.5	—	pH 7.5, 60 °C	Open record: aspartate kinase, adenosine 5'-triphosphate
aspartate kinase	Thermus flavus	L-aspartic acid	25	0.27	93 calc.	pH 7.5, 60 °C	Open record: aspartate kinase, L-aspartic acid
aspartate kinasevariant A157L	Thermus flavus	adenosine 5'-triphosphate	—	0.45	—	pH 7.5, 60 °C	Open record: aspartate kinase variant A157L, adenosine 5'-triphosphate
aspartate kinasevariant A157L	Thermus flavus	L-aspartic acid	4.7	0.19	25 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant A157L, L-aspartic acid
aspartate kinasevariant A189L	Thermus flavus	adenosine 5'-triphosphate	—	0.98	—	pH 7.5, 60 °C	Open record: aspartate kinase variant A189L, adenosine 5'-triphosphate
aspartate kinasevariant A189L	Thermus flavus	L-aspartic acid	6	0.45	13 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant A189L, L-aspartic acid
aspartate kinasevariant A23L	Thermus flavus	adenosine 5'-triphosphate	—	0.72	—	pH 7.5, 60 °C	Open record: aspartate kinase variant A23L, adenosine 5'-triphosphate
aspartate kinasevariant A23L	Thermus flavus	L-aspartic acid	24.3	0.65	37 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant A23L, L-aspartic acid
aspartate kinasevariant A42S	Thermus flavus	adenosine 5'-triphosphate	—	1.02	—	pH 7.5, 60 °C	Open record: aspartate kinase variant A42S, adenosine 5'-triphosphate
aspartate kinasevariant A42S	Thermus flavus	L-aspartic acid	23.2	0.6	39 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant A42S, L-aspartic acid
aspartate kinasevariant D154A	Thermus flavus	adenosine 5'-triphosphate	—	1.05	—	pH 7.5, 60 °C	Open record: aspartate kinase variant D154A, adenosine 5'-triphosphate
aspartate kinasevariant D154A	Thermus flavus	L-aspartic acid	1.8	1.2	1.5 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant D154A, L-aspartic acid
aspartate kinasevariant D154N	Thermus flavus	adenosine 5'-triphosphate	—	0.63	—	pH 7.5, 60 °C	Open record: aspartate kinase variant D154N, adenosine 5'-triphosphate
aspartate kinasevariant D154N	Thermus flavus	L-aspartic acid	2.17	0.47	4.6 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant D154N, L-aspartic acid
aspartate kinasevariant D174A	Thermus flavus	adenosine 5'-triphosphate	—	0.43	—	pH 7.5, 60 °C	Open record: aspartate kinase variant D174A, adenosine 5'-triphosphate
aspartate kinasevariant D174A	Thermus flavus	L-aspartic acid	3	0.53	5.7 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant D174A, L-aspartic acid
aspartate kinasevariant D182A	Thermus flavus	adenosine 5'-triphosphate	—	2.45	—	pH 7.5, 60 °C	Open record: aspartate kinase variant D182A, adenosine 5'-triphosphate
aspartate kinasevariant D182A	Thermus flavus	L-aspartic acid	2.2	1.31	1.7 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant D182A, L-aspartic acid
aspartate kinasevariant E202A	Thermus flavus	adenosine 5'-triphosphate	—	0.29	—	pH 7.5, 60 °C	Open record: aspartate kinase variant E202A, adenosine 5'-triphosphate
aspartate kinasevariant E202A	Thermus flavus	L-aspartic acid	4	0.27	15 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant E202A, L-aspartic acid
aspartate kinasevariant F136A	Thermus flavus	adenosine 5'-triphosphate	—	0.57	—	pH 7.5, 60 °C	Open record: aspartate kinase variant F136A, adenosine 5'-triphosphate
aspartate kinasevariant F136A	Thermus flavus	L-aspartic acid	17	5.29	3.2 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant F136A, L-aspartic acid
aspartate kinasevariant G10A	Thermus flavus	adenosine 5'-triphosphate	—	3.57	—	pH 7.5, 60 °C	Open record: aspartate kinase variant G10A, adenosine 5'-triphosphate
aspartate kinasevariant G10A	Thermus flavus	L-aspartic acid	2.8	1.34	2.1 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant G10A, L-aspartic acid
aspartate kinasevariant G135A	Thermus flavus	adenosine 5'-triphosphate	—	0.52	—	pH 7.5, 60 °C	Open record: aspartate kinase variant G135A, adenosine 5'-triphosphate
aspartate kinasevariant G135A	Thermus flavus	L-aspartic acid	0.97	1.34	0.72 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant G135A, L-aspartic acid
aspartate kinasevariant G149A	Thermus flavus	adenosine 5'-triphosphate	—	0.41	—	pH 7.5, 60 °C	Open record: aspartate kinase variant G149A, adenosine 5'-triphosphate
aspartate kinasevariant G149A	Thermus flavus	L-aspartic acid	13.3	0.36	37 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant G149A, L-aspartic acid
aspartate kinasevariant G152A	Thermus flavus	adenosine 5'-triphosphate	—	0.5	—	pH 7.5, 60 °C	Open record: aspartate kinase variant G152A, adenosine 5'-triphosphate
aspartate kinasevariant G152A	Thermus flavus	L-aspartic acid	4.3	0.42	10 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant G152A, L-aspartic acid
aspartate kinasevariant G177A	Thermus flavus	adenosine 5'-triphosphate	—	0.27	—	pH 7.5, 60 °C	Open record: aspartate kinase variant G177A, adenosine 5'-triphosphate
aspartate kinasevariant G177A	Thermus flavus	L-aspartic acid	13.3	0.34	39 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant G177A, L-aspartic acid
aspartate kinasevariant G73A	Thermus flavus	adenosine 5'-triphosphate	—	1.28	—	pH 7.5, 60 °C	Open record: aspartate kinase variant G73A, adenosine 5'-triphosphate
aspartate kinasevariant G73A	Thermus flavus	L-aspartic acid	32	0.61	52 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant G73A, L-aspartic acid
aspartate kinasevariant I171A	Thermus flavus	adenosine 5'-triphosphate	—	0.6	—	pH 7.5, 60 °C	Open record: aspartate kinase variant I171A, adenosine 5'-triphosphate
aspartate kinasevariant I171A	Thermus flavus	L-aspartic acid	10.8	0.37	29 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant I171A, L-aspartic acid
aspartate kinasevariant L148A	Thermus flavus	adenosine 5'-triphosphate	—	0.97	—	pH 7.5, 60 °C	Open record: aspartate kinase variant L148A, adenosine 5'-triphosphate
aspartate kinasevariant L148A	Thermus flavus	L-aspartic acid	23	2.1	11 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant L148A, L-aspartic acid
aspartate kinasevariant P183A	Thermus flavus	adenosine 5'-triphosphate	—	1.9	—	pH 7.5, 60 °C	Open record: aspartate kinase variant P183A, adenosine 5'-triphosphate
aspartate kinasevariant P183A	Thermus flavus	L-aspartic acid	4.3	1.2	3.6 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant P183A, L-aspartic acid
aspartate kinasevariant R150A	Thermus flavus	adenosine 5'-triphosphate	—	0.46	—	pH 7.5, 60 °C	Open record: aspartate kinase variant R150A, adenosine 5'-triphosphate
aspartate kinasevariant R150A	Thermus flavus	L-aspartic acid	1.8	1.23	1.5 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant R150A, L-aspartic acid
aspartate kinasevariant S12A	Thermus flavus	adenosine 5'-triphosphate	—	0.52	—	pH 7.5, 60 °C	Open record: aspartate kinase variant S12A, adenosine 5'-triphosphate
aspartate kinasevariant S12A	Thermus flavus	L-aspartic acid	24.7	0.32	77 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant S12A, L-aspartic acid
aspartate kinasevariant S153A	Thermus flavus	adenosine 5'-triphosphate	—	0.44	—	pH 7.5, 60 °C	Open record: aspartate kinase variant S153A, adenosine 5'-triphosphate
aspartate kinasevariant S153A	Thermus flavus	L-aspartic acid	12.3	0.47	26 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant S153A, L-aspartic acid
aspartate kinasevariant S41A	Thermus flavus	adenosine 5'-triphosphate	—	1.39	—	pH 7.5, 60 °C	Open record: aspartate kinase variant S41A, adenosine 5'-triphosphate
aspartate kinasevariant S41A	Thermus flavus	L-aspartic acid	0.8	0.41	2.0 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant S41A, L-aspartic acid
aspartate kinasevariant T238A	Thermus flavus	adenosine 5'-triphosphate	—	0.56	—	pH 7.5, 60 °C	Open record: aspartate kinase variant T238A, adenosine 5'-triphosphate
aspartate kinasevariant T238A	Thermus flavus	L-aspartic acid	7.3	0.39	19 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant T238A, L-aspartic acid
aspartate kinasevariant T47A	Thermus flavus	adenosine 5'-triphosphate	—	2.1	—	pH 7.5, 60 °C	Open record: aspartate kinase variant T47A, adenosine 5'-triphosphate
aspartate kinasevariant T47A	Thermus flavus	L-aspartic acid	6.7	19.5	0.34 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant T47A, L-aspartic acid
aspartate kinasevariant V39A	Thermus flavus	adenosine 5'-triphosphate	—	0.89	—	pH 7.5, 60 °C	Open record: aspartate kinase variant V39A, adenosine 5'-triphosphate
aspartate kinasevariant V39A	Thermus flavus	L-aspartic acid	32	0.33	97 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant V39A, L-aspartic acid
aspartate kinasevariant Y8A	Thermus flavus	adenosine 5'-triphosphate	—	0.86	—	pH 7.5, 60 °C	Open record: aspartate kinase variant Y8A, adenosine 5'-triphosphate
aspartate kinasevariant Y8A	Thermus flavus	L-aspartic acid	13.8	0.32	43 calc.	pH 7.5, 60 °C	Open record: aspartate kinase variant Y8A, L-aspartic acid