Values CatLog read from this paper
Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis
PMID 16332250doi:10.1042/bj20051525Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (13 values) from this paper: methylmalonate semialdehyde dehydrogenase (MSDH) from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 3 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | coenzyme Acosubstrate MMSA, NAD | — | 0.12 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | coenzyme Acosubstrate MSA, NAD | — | 0.03 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | malonate semialdehyde | 7.4 | 0.12 | 62 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), malonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | methylmalonate semialdehydekcat per subunit | 1.1 | 0.06 | 18 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | methylmalonate semialdehydekcat per active site | 2.2 | — | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | NAD+cosubstrate MMSA, CoA | — | 2.3 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | NAD+cosubstrate MSA, CoA | — | 1.78 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant C49A/C176A/C305A/C369A/C403A | Bacillus subtilis | coenzyme A | — | 0.1 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant C49A/C176A/C305A/C369A/C403A, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant C49A/C176A/C305A/C369A/C403A | Bacillus subtilis | methylmalonate semialdehyde | 1.25 | 0.17 | 7.4 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant C49A/C176A/C305A/C369A/C403A, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant C49A/C176A/C305A/C369A/C403A | Bacillus subtilis | NAD+ | — | 1.22 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant C49A/C176A/C305A/C369A/C403A, NAD+ |