EC 1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA-acylating)
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CatLog read 43 records for EC 1.2.1.27 from 5 papers, for 4 organisms. Names in these papers: methylmalonate semialdehyde dehydrogenase (MSDH), methylmalonate semialdehyde dehydrogenase, methylmalonate-semialdehyde dehydrogenase (MMSDH).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 11 records for EC 1.2.1.27 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 1.2.1.27 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Adenine binding mode is a key factor in triggering the early release of NADH in coenzyme A-dependent methylmalonate semialdehyde dehydrogenase | Bacillus subtilis | 18 |
| Mechanistic characterization of the MSDH (methylmalonate semialdehyde dehydrogenase) from Bacillus subtilis | Bacillus subtilis | 10 |
| Properties of purified methylmalonate semialdehyde dehydrogenase of Pseudomonas aeruginosa | Pseudomonas aeruginosa | 8 |
| Purification and characterization of methylmalonate-semialdehyde dehydrogenase from rat liver. Identity to malonate-semialdehyde dehydrogenase | Rattus norvegicus | 4 |
| Unraveling the function of paralogs of the aldehyde dehydrogenase super family from Sulfolobus solfataricus | Sulfolobus solfataricus | 3 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | 2-mercaptoethanol | — | 0.054 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, 2-mercaptoethanol |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | coenzyme A | — | 0.0022 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, coenzyme A |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | methylmalonate semialdehydeacyl acceptor CoA; cosubstrate NAD; CoA | — | 0.0019 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | methylmalonate semialdehydeacyl acceptor 2-mercaptoethanol (replacing CoA) | — | 0.0035 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | NAD+acyl acceptor CoA; CoA | — | 0.0082 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, NAD+ |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | NAD+acyl acceptor 2-mercaptoethanol (replacing CoA) | — | 0.0087 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, NAD+ |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | propanalacyl acceptor CoA; 0.03 unit enzyme per ml | — | 0.61 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, propanal |
| methylmalonate semialdehyde dehydrogenase | Pseudomonas aeruginosa | propanalacyl acceptor 2-mercaptoethanol (replacing CoA) | — | 5.11 | — | pH 9.2, 37 °C | PMID 4314598 | Open record: methylmalonate semialdehyde dehydrogenase, propanal |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | coenzyme Acosubstrate MMSA, NAD | — | 0.12 | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | coenzyme Acosubstrate MSA, NAD | — | 0.03 | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | malonate semialdehyde | 7.4 | 0.12 | 62 calc. | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), malonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | methylmalonate semialdehydekcat per subunit | 1.1 | 0.06 | 18 calc. | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | methylmalonate semialdehydekcat per active site | 2.2 | — | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | NAD+cosubstrate MMSA, CoA | — | 2.3 | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Bacillus subtilis | NAD+cosubstrate MSA, CoA | — | 1.78 | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant ::G225 | Bacillus subtilis | coenzyme A | — | 0.151 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant ::G225, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant ::G225 | Bacillus subtilis | methylmalonate semialdehyde | 0.39 | 0.022 | 18 calc. | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant ::G225, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant ::G225 | Bacillus subtilis | NAD+ | — | 0.69 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant ::G225, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant C49A/C176A/C305A/C369A/C403A | Bacillus subtilis | coenzyme A | — | 0.1 | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant C49A/C176A/C305A/C369A/C403A, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant C49A/C176A/C305A/C369A/C403A | Bacillus subtilis | methylmalonate semialdehyde | 1.25 | 0.17 | 7.4 calc. | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant C49A/C176A/C305A/C369A/C403A, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant C49A/C176A/C305A/C369A/C403A | Bacillus subtilis | NAD+ | — | 1.22 | — | pH 8.2, 30 °C | PMID 16332250 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant C49A/C176A/C305A/C369A/C403A, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G | Bacillus subtilis | coenzyme A | — | 0.234 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G | Bacillus subtilis | methylmalonate semialdehyde | 0.78 | 0.054 | 14 calc. | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G | Bacillus subtilis | NAD+ | — | 0.57 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225 | Bacillus subtilis | coenzyme A | — | 0.063 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225 | Bacillus subtilis | methylmalonate semialdehyde | 0.21 | 0.027 | 7.8 calc. | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225 | Bacillus subtilis | NAD+ | — | 0.15 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/H226P | Bacillus subtilis | coenzyme A | — | 0.096 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/H226P, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/H226P | Bacillus subtilis | methylmalonate semialdehyde | 0.02 | 0.021 | 0.95 calc. | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/H226P, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/H226P | Bacillus subtilis | NAD+ | — | 0.77 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/H226P, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/Y252L/V253I | Bacillus subtilis | coenzyme A | — | 0.497 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/Y252L/V253I, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/Y252L/V253I | Bacillus subtilis | methylmalonate semialdehyde | 0.02 | 0.215 | 0.093 calc. | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/Y252L/V253I, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/Y252L/V253I | Bacillus subtilis | NAD+ | — | 9.44 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/Y252L/V253I, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/H226P | Bacillus subtilis | coenzyme A | — | 0.119 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/H226P, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/H226P | Bacillus subtilis | methylmalonate semialdehyde | 0.09 | 0.028 | 3.2 calc. | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/H226P, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/H226P | Bacillus subtilis | NAD+ | — | 0.66 | — | pH 8.2, 30 °C | PMID 22782904 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/H226P, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Sulfolobus solfataricus | D,L-methylmalonate semialdehyde | 0.003 | — | — | pH 8.2, 30 °C | PMID 23296511 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), D,L-methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Sulfolobus solfataricus | malonate semialdehyde | 0.02 | — | — | pH 8.2, 30 °C | PMID 23296511 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), malonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH) | Sulfolobus solfataricus | propionaldehyde | 0.03 | — | — | pH 8.2, 30 °C | PMID 23296511 | Open record: methylmalonate semialdehyde dehydrogenase (MSDH), propionaldehyde |
| methylmalonate-semialdehyde dehydrogenase (MMSDH) | Rattus norvegicus | 2-methyl-3-oxopropanoate | — | 0.0053 | — | PMID 2768248 | Open record: methylmalonate-semialdehyde dehydrogenase (MMSDH), 2-methyl-3-oxopropanoate | |
| methylmalonate-semialdehyde dehydrogenase (MMSDH) | Rattus norvegicus | coenzyme A | — | 0.03 | — | PMID 2768248 | Open record: methylmalonate-semialdehyde dehydrogenase (MMSDH), coenzyme A | |
| methylmalonate-semialdehyde dehydrogenase (MMSDH) | Rattus norvegicus | malonate semialdehyde | — | 0.0045 | — | PMID 2768248 | Open record: methylmalonate-semialdehyde dehydrogenase (MMSDH), malonate semialdehyde | |
| methylmalonate-semialdehyde dehydrogenase (MMSDH) | Rattus norvegicus | NAD+ | — | 0.15 | — | PMID 2768248 | Open record: methylmalonate-semialdehyde dehydrogenase (MMSDH), NAD+ |