Values CatLog read from this paper
Adenine binding mode is a key factor in triggering the early release of NADH in coenzyme A-dependent methylmalonate semialdehyde dehydrogenase
PMID 22782904doi:10.1074/jbc.m112.350272Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (24 values) from this paper: methylmalonate semialdehyde dehydrogenase (MSDH) from Bacillus subtilis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| methylmalonate semialdehyde dehydrogenase (MSDH)variant ::G225 | Bacillus subtilis | coenzyme A | — | 0.151 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant ::G225, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant ::G225 | Bacillus subtilis | methylmalonate semialdehyde | 0.39 | 0.022 | 18 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant ::G225, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant ::G225 | Bacillus subtilis | NAD+ | — | 0.69 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant ::G225, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G | Bacillus subtilis | coenzyme A | — | 0.234 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G | Bacillus subtilis | methylmalonate semialdehyde | 0.78 | 0.054 | 14 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G | Bacillus subtilis | NAD+ | — | 0.57 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225 | Bacillus subtilis | coenzyme A | — | 0.063 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225 | Bacillus subtilis | methylmalonate semialdehyde | 0.21 | 0.027 | 7.8 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225 | Bacillus subtilis | NAD+ | — | 0.15 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/H226P | Bacillus subtilis | coenzyme A | — | 0.096 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/H226P, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/H226P | Bacillus subtilis | methylmalonate semialdehyde | 0.02 | 0.021 | 0.95 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/H226P, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/H226P | Bacillus subtilis | NAD+ | — | 0.77 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/H226P, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/Y252L/V253I | Bacillus subtilis | coenzyme A | — | 0.497 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/Y252L/V253I, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/Y252L/V253I | Bacillus subtilis | methylmalonate semialdehyde | 0.02 | 0.215 | 0.093 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/Y252L/V253I, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/::G225/Y252L/V253I | Bacillus subtilis | NAD+ | — | 9.44 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/::G225/Y252L/V253I, NAD+ |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/H226P | Bacillus subtilis | coenzyme A | — | 0.119 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/H226P, coenzyme A |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/H226P | Bacillus subtilis | methylmalonate semialdehyde | 0.09 | 0.028 | 3.2 calc. | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/H226P, methylmalonate semialdehyde |
| methylmalonate semialdehyde dehydrogenase (MSDH)variant V229G/H226P | Bacillus subtilis | NAD+ | — | 0.66 | — | pH 8.2, 30 °C | Open record: methylmalonate semialdehyde dehydrogenase (MSDH) variant V229G/H226P, NAD+ |