Values CatLog read from this paper
Probing the substrate binding site of Candida tenuis xylose reductase (AKR2B5) with site-directed mutagenesis
PMID 16336198doi:10.1042/bj20050831Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 62 records (124 values) from this paper: xylose reductase (CtXR, AKR2B5) from Candida tenuis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | 2-deoxy-D-galactose | 2.1 | 742 | 9.3 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), 2-deoxy-D-galactose |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | 3-hydroxybutan-2-one | 0.8 | 451 | 0.0018 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), 3-hydroxybutan-2-one |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | 4,4-dimethyloxolane-2,3-dione | — | — | 0.008 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), 4,4-dimethyloxolane-2,3-dione |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | 4-Hydroxybutan-2-one | 0.02 | 392 | 5 × 10⁻⁵ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), 4-Hydroxybutan-2-one |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | Acetophenone | — | — | 5 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), Acetophenone |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | butane-2,3-dione | 12 | 15 | 0.8 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), butane-2,3-dione |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | D-galactose | 11 | 208 | 265 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), D-galactose |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | D-glucose | 4.8 | 236 | 833 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), D-glucose |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | D-xyloseNADH-dependent reduction | 12.4 | 91 | 680 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), D-xylose |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | D-xyloseNADH-dependent xylose reduction | 11 | 142 | 0.077 calc. | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), D-xylose |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | DL-glyceraldehyde | 7.8 | 3.5 | 8.9 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), DL-glyceraldehyde |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | ethyl 2-oxo-2-phenylacetate | — | — | 0.269 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), ethyl 2-oxo-2-phenylacetate |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | ethyl 2-oxopropanoate | 6.5 | 12 | 0.542 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), ethyl 2-oxopropanoate |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | ethyl 3-oxobutanoate | — | — | 7 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), ethyl 3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | ethyl 4,4,4-trifluoro-3-oxobutanoate | — | — | 3 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), ethyl 4,4,4-trifluoro-3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | ethyl 4-chloro-3-oxobutanoate | — | — | 0.034 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), ethyl 4-chloro-3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | NADH | — | 0.038 | — | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), NADH |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | NADPH | 19 | 0.0032 | 5.9 × 10³ calc. | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), NADPH |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | pentane-2,4-dione | — | — | 7 × 10⁻⁵ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), pentane-2,4-dione |
| xylose reductase (CtXR, AKR2B5) | Candida tenuis | xylitol | 1.1 | 334 | 0.0033 calc. | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5), xylitol |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | 2-deoxy-D-galactose | — | — | 6.3 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, 2-deoxy-D-galactose |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | D-galactose | 3.3 | 244 | 70 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, D-galactose |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | D-glucose | 2.2 | 486 | 188 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, D-glucose |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | D-xyloseNADH-dependent reduction | 3.4 | 243 | 14 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, D-xylose |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | D-xyloseNADH-dependent xylose reduction | 3.4 | 158 | 0.022 calc. | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, D-xylose |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | NADH | — | 0.04 | — | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, NADH |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | NADPH | 11 | 0.0023 | 4.8 × 10³ calc. | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, NADPH |
| xylose reductase (CtXR, AKR2B5)variant D50A | Candida tenuis | xylitol | 0.2 | 537 | 3.7 × 10⁻⁴ calc. | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant D50A, xylitol |
| xylose reductase (CtXR, AKR2B5)variant N309A | Candida tenuis | 2-deoxy-D-galactose | 0.9 | 178 | 17 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309A, 2-deoxy-D-galactose |
| xylose reductase (CtXR, AKR2B5)variant N309A | Candida tenuis | D-galactose | — | — | 9 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309A, D-galactose |
| xylose reductase (CtXR, AKR2B5)variant N309A | Candida tenuis | D-glucose | — | — | 8.3 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309A, D-glucose |
| xylose reductase (CtXR, AKR2B5)variant N309A | Candida tenuis | D-xylose | 6.8 | 1787 | 19 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309A, D-xylose |
| xylose reductase (CtXR, AKR2B5)variant N309A | Candida tenuis | DL-glyceraldehyde | 4.2 | 54 | 0.3 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309A, DL-glyceraldehyde |
| xylose reductase (CtXR, AKR2B5)variant N309D | Candida tenuis | 2-deoxy-D-galactose | 0.2 | 187 | 3.7 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309D, 2-deoxy-D-galactose |
| xylose reductase (CtXR, AKR2B5)variant N309D | Candida tenuis | D-galactose | 0.3 | 264 | 5.5 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309D, D-galactose |
| xylose reductase (CtXR, AKR2B5)variant N309D | Candida tenuis | D-glucose | — | — | 8.3 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309D, D-glucose |
| xylose reductase (CtXR, AKR2B5)variant N309D | Candida tenuis | D-xylose | 2.4 | 1349 | 9 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309D, D-xylose |
| xylose reductase (CtXR, AKR2B5)variant N309D | Candida tenuis | DL-glyceraldehyde | 2.3 | 35 | 0.3 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant N309D, DL-glyceraldehyde |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | 3-hydroxybutan-2-one | 0.7 | 851 | 8 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, 3-hydroxybutan-2-one |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | 4,4-dimethyloxolane-2,3-dione | — | — | 0.046 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, 4,4-dimethyloxolane-2,3-dione |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | 4-Hydroxybutan-2-one | 0.02 | 373 | 5 × 10⁻⁵ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, 4-Hydroxybutan-2-one |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | Acetophenone | — | — | 2 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, Acetophenone |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | butane-2,3-dione | 9.6 | 34 | 0.282 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, butane-2,3-dione |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | D-xylose | 7.4 | 1475 | 25 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, D-xylose |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | ethyl 2-oxo-2-phenylacetate | — | — | 0.911 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, ethyl 2-oxo-2-phenylacetate |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | ethyl 2-oxopropanoate | 2.4 | 15 | 0.16 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, ethyl 2-oxopropanoate |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | ethyl 3-oxobutanoate | — | — | 3 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, ethyl 3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | ethyl 4,4,4-trifluoro-3-oxobutanoate | — | — | 6 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, ethyl 4,4,4-trifluoro-3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | ethyl 4-chloro-3-oxobutanoate | — | — | 0.068 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, ethyl 4-chloro-3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5)variant W23F | Candida tenuis | pentane-2,4-dione | — | — | 3 × 10⁻⁵ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23F, pentane-2,4-dione |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | 3-hydroxybutan-2-one | 0.4 | 519 | 8 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, 3-hydroxybutan-2-one |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | 4,4-dimethyloxolane-2,3-dione | — | — | 0.036 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, 4,4-dimethyloxolane-2,3-dione |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | 4-Hydroxybutan-2-one | 0.01 | 289 | 3 × 10⁻⁵ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, 4-Hydroxybutan-2-one |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | Acetophenone | — | — | 2 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, Acetophenone |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | butane-2,3-dione | 13 | 107 | 0.121 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, butane-2,3-dione |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | D-xylose | — | — | 6.5 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, D-xylose |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | ethyl 2-oxo-2-phenylacetate | — | — | 0.636 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, ethyl 2-oxo-2-phenylacetate |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | ethyl 2-oxopropanoate | 0.8 | 14 | 0.057 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, ethyl 2-oxopropanoate |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | ethyl 3-oxobutanoate | — | — | 2 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, ethyl 3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | ethyl 4,4,4-trifluoro-3-oxobutanoate | — | — | 3 × 10⁻⁴ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, ethyl 4,4,4-trifluoro-3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | ethyl 4-chloro-3-oxobutanoate | — | — | 0.062 | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, ethyl 4-chloro-3-oxobutanoate |
| xylose reductase (CtXR, AKR2B5)variant W23Y | Candida tenuis | pentane-2,4-dione | — | — | 3 × 10⁻⁵ | pH 7, 25 °C | Open record: xylose reductase (CtXR, AKR2B5) variant W23Y, pentane-2,4-dione |