CatLogenzyme kinetics catalog

Values CatLog read from this paper

Crystal structure of type-III geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae and the mechanism of product chain length determination

PMID 16554305doi:10.1074/jbc.m512886200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (36 values) from this paper: geranylgeranyl pyrophosphate synthase (type-III GGPPs) from Saccharomyces cerevisiae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 12 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.5.1.1, EC 2.5.1.10, EC 2.5.1.29

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
geranylgeranyl pyrophosphate synthase (type-III GGPPs)Saccharomyces cerevisiaefarnesyl pyrophosphate0.0250.00327.8 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)Saccharomyces cerevisiaegeranyl pyrophosphate0.0139 × 10⁻⁴14 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)Saccharomyces cerevisiaeisopentenyl pyrophosphateIPP varied with 20 µM FPP (saturating); cosubstrate FPP—8 × 10⁻⁴—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)Saccharomyces cerevisiaeisopentenyl pyrophosphateIPP varied with GPP as allylic substrate; cosubstrate GPP—0.0013—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant F108ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0470.002122 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant F108ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.0027—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant F108A/H139ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0160.00513.1 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant F108A/H139ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.136—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant H139ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0420.002517 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant H139ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.0051—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant L135A/H139ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0640.002724 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant L135A/H139ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.017—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant S71YSaccharomyces cerevisiaegeranyl pyrophosphate0.00340.00172.0 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant S71YSaccharomyces cerevisiaeisopentenyl pyrophosphate—0.169—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0560.001733 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.0047—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/F108ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0490.002619 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/F108ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.0033—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/F108A/H139ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0254 × 10⁻⁴62 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/F108A/H139ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.097—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/F108A/L135A/H139ASaccharomyces cerevisiaefarnesyl pyrophosphate0.00840.0018.4 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/F108A/L135A/H139ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.051—pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/H139ASaccharomyces cerevisiaefarnesyl pyrophosphate0.0390.001135 calc.pH 7.5, 25 °COpen
geranylgeranyl pyrophosphate synthase (type-III GGPPs)variant Y107A/H139ASaccharomyces cerevisiaeisopentenyl pyrophosphate—0.267—pH 7.5, 25 °COpen