Values CatLog read from this paper
Catalysis by the second class of tRNA(m1G37) methyl transferase requires a conserved proline
PMID 16768442doi:10.1021/bi0602314Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 15 records (45 values) from this paper: tRNA(m1G37) methyl transferase Trm5 from Methanocaldococcus jannaschii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| tRNA(m1G37) methyl transferase Trm5 | Methanocaldococcus jannaschii | S-adenosyl methionine | 0.0122 | 0.001 | 12 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5, S-adenosyl methionine |
| tRNA(m1G37) methyl transferase Trm5 | Methanocaldococcus jannaschii | tRNACys | 0.0083 | 7 × 10⁻⁴ | 12 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant D223A | Methanocaldococcus jannaschii | tRNACys | 3.3 × 10⁻⁴ | 0.003 | 0.13 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant D223A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant G205A/G207A | Methanocaldococcus jannaschii | tRNACys | 0.0015 | 0.0046 | 0.33 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant G205A/G207A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant N225A | Methanocaldococcus jannaschii | tRNACys | 0.0037 | 0.0072 | 0.5 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant N225A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant N265A | Methanocaldococcus jannaschii | tRNACys | 0.0013 | 0.0083 | 0.17 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant N265A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant N265H | Methanocaldococcus jannaschii | tRNACys | 0.0033 | 8 × 10⁻⁴ | 4.2 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant N265H, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant N265H/P267A | Methanocaldococcus jannaschii | tRNACys | 1 × 10⁻⁴ | 0.0051 | 0.017 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant N265H/P267A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant N265Q | Methanocaldococcus jannaschii | tRNACys | 5 × 10⁻⁴ | 0.0032 | 0.18 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant N265Q, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant N265Q/P267A | Methanocaldococcus jannaschii | tRNACys | 1.2 × 10⁻⁴ | 0.0042 | 0.017 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant N265Q/P267A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant P226A | Methanocaldococcus jannaschii | tRNACys | 0.0018 | 0.003 | 0.63 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant P226A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant P267A | Methanocaldococcus jannaschii | S-adenosyl methionine | 1 × 10⁻⁴ | 5 × 10⁻⁴ | 0.17 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant P267A, S-adenosyl methionine |
| tRNA(m1G37) methyl transferase Trm5variant P267A | Methanocaldococcus jannaschii | tRNACys | 1 × 10⁻⁴ | 0.006 | 0.017 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant P267A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant R144A | Methanocaldococcus jannaschii | tRNACys | 0.0033 | 0.0043 | 0.67 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant R144A, tRNACys |
| tRNA(m1G37) methyl transferase Trm5variant Y176A | Methanocaldococcus jannaschii | tRNACys | 0.0013 | 0.0025 | 0.5 | pH 8, 50 °C | Open record: tRNA(m1G37) methyl transferase Trm5 variant Y176A, tRNACys |