CatLogenzyme kinetics catalog

EC 2.1.1.228: tRNA (guanine37-N1)-methyltransferase

TransferasesOpen in Browse

CatLog read 48 records for EC 2.1.1.228 from 3 papers, for 4 organisms. Names in these papers: tRNA-(N1G37) methyltransferase TRM5, tRNA(m1G37) methyl transferase Trm5, TrmD (tRNA m1G37 methyltransferase), tRNA-(N1G37) methyltransferase TrmD.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.

CatLog also holds 7 records for EC 2.1.1.228 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 2.1.1.228 in Browse.

Papers

PaperOrganismsRecords
Isolation and characterization of the human tRNA-(N1G37) methyltransferase (TRM5) and comparison to the Escherichia coli TrmD proteinEscherichia coli, Homo sapiens23
Catalysis by the second class of tRNA(m1G37) methyl transferase requires a conserved prolineMethanocaldococcus jannaschii15
Structural basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmDHaemophilus influenzae10

Records

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)ConditionsPaper
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeHaemophilus influenzae tRNAArg in vitro transcript0.0780.0272.9pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeHaemophilus influenzae tRNALeu in vitro transcript0.320.00840pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeHaemophilus influenzae tRNAPro in vitro transcript0.130.001130pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, G36A variant0.360.0027130pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, G36C variant0.360.1842pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, G36U variant0.170.1141.5pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)Haemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, wild type0.248.2 × 10⁻⁴290pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)variant D169AHaemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, wild type0.00470.0670.07pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)variant R154AHaemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, wild type0.00140.0640.022pH 8PMID 26183229Open
TrmD (tRNA m1G37 methyltransferase)variant S165AHaemophilus influenzaeThermotoga maritima tRNAGln(CUG) in vitro transcript, wild type0.340.004478pH 8PMID 26183229Open
tRNA(m1G37) methyl transferase Trm5Methanocaldococcus jannaschiiS-adenosyl methionine0.01220.00112pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5Methanocaldococcus jannaschiitRNACys0.00837 × 10⁻⁴12pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant D223AMethanocaldococcus jannaschiitRNACys3.3 × 10⁻⁴0.0030.13pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant G205A/G207AMethanocaldococcus jannaschiitRNACys0.00150.00460.33pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant N225AMethanocaldococcus jannaschiitRNACys0.00370.00720.5pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant N265AMethanocaldococcus jannaschiitRNACys0.00130.00830.17pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant N265HMethanocaldococcus jannaschiitRNACys0.00338 × 10⁻⁴4.2pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant N265H/P267AMethanocaldococcus jannaschiitRNACys1 × 10⁻⁴0.00510.017pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant N265QMethanocaldococcus jannaschiitRNACys5 × 10⁻⁴0.00320.18pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant N265Q/P267AMethanocaldococcus jannaschiitRNACys1.2 × 10⁻⁴0.00420.017pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant P226AMethanocaldococcus jannaschiitRNACys0.00180.0030.63pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant P267AMethanocaldococcus jannaschiiS-adenosyl methionine1 × 10⁻⁴5 × 10⁻⁴0.17pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant P267AMethanocaldococcus jannaschiitRNACys1 × 10⁻⁴0.0060.017pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant R144AMethanocaldococcus jannaschiitRNACys0.00330.00430.67pH 8, 50 °CPMID 16768442Open
tRNA(m1G37) methyl transferase Trm5variant Y176AMethanocaldococcus jannaschiitRNACys0.00130.00250.5pH 8, 50 °CPMID 16768442Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensE. coli tRNALeu1 transcript—5.46 × 10⁻⁵—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensE. coli tRNALeu1 transcript + 3 bp in acceptor stem + 3' extension—5.67 × 10⁻⁵—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensE. coli tRNALeu1 transcript G19A (has C36)—0.00597—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensE. coli tRNALeu1 transcript minus V-stem-loop—0.00619—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensE. coli tRNALeu1 transcript with bulge in acceptor stem—4.99 × 10⁻⁵—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapienshuman mitochondrial tRNAPro transcript—0.00546—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapienshuman mitochondrial tRNAPro transcript A36—0.018—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensS-adenosyl-L-methionine(extrapolated to zero)—0.0028—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensS-adenosyl-L-methionine(saturating, extrapolated)—0.0011—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensSalmonella typhimurium GT875 bulk tRNAs (m1G37-deficient)—0.00527—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript A36—5.9 × 10⁻⁴—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript C36 (Asp wild-type, C36pG37)—7.5 × 10⁻⁵—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript U36—5.8 × 10⁻⁴—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript with extended D-stem—0.0026—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript with G36 (G36pG37)—2.44 × 10⁻⁴—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript with G36 (tRNAAspGG)(extrapolated to zero)—1.63 × 10⁻⁴—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAAsp transcript with G36 (tRNAAspGG)(saturating, extrapolated)—6.5 × 10⁻⁵—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TRM5Homo sapiensyeast tRNAPhe transcript (A36)—4.57 × 10⁻⁵—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TrmDEscherichia coliE. coli tRNALeu1 transcript—0.0057—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TrmDEscherichia colihuman mitochondrial tRNAPro transcript—0.0086—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TrmDEscherichia coliyeast tRNAAsp transcript A36—0.046—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TrmDEscherichia coliyeast tRNAAsp transcript with G36 (G36pG37)—0.019—pH 7.34, 37 °CPMID 15248782Open
tRNA-(N1G37) methyltransferase TrmDEscherichia coliyeast tRNAPhe transcript (A36)—0.01—pH 7.34, 37 °CPMID 15248782Open