Values CatLog read from this paper
Structural basis for methyl-donor-dependent and sequence-specific binding to tRNA substrates by knotted methyltransferase TrmD
PMID 26183229doi:10.1073/pnas.1422981112Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (30 values) from this paper: TrmD (tRNA m1G37 methyltransferase) from Haemophilus influenzae.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Haemophilus influenzae tRNAArg in vitro transcript | 0.078 | 0.027 | 2.9 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Haemophilus influenzae tRNAArg in vitro transcript |
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Haemophilus influenzae tRNALeu in vitro transcript | 0.32 | 0.008 | 40 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Haemophilus influenzae tRNALeu in vitro transcript |
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Haemophilus influenzae tRNAPro in vitro transcript | 0.13 | 0.001 | 130 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Haemophilus influenzae tRNAPro in vitro transcript |
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, G36A variant | 0.36 | 0.0027 | 130 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Thermotoga maritima tRNAGln(CUG) in vitro transcript, G36A variant |
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, G36C variant | 0.36 | 0.184 | 2 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Thermotoga maritima tRNAGln(CUG) in vitro transcript, G36C variant |
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, G36U variant | 0.17 | 0.114 | 1.5 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Thermotoga maritima tRNAGln(CUG) in vitro transcript, G36U variant |
| TrmD (tRNA m1G37 methyltransferase) | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type | 0.24 | 8.2 × 10⁻⁴ | 290 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase), Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type |
| TrmD (tRNA m1G37 methyltransferase)variant D169A | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type | 0.0047 | 0.067 | 0.07 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase) variant D169A, Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type |
| TrmD (tRNA m1G37 methyltransferase)variant R154A | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type | 0.0014 | 0.064 | 0.022 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase) variant R154A, Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type |
| TrmD (tRNA m1G37 methyltransferase)variant S165A | Haemophilus influenzae | Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type | 0.34 | 0.0044 | 78 | pH 8 | Open record: TrmD (tRNA m1G37 methyltransferase) variant S165A, Thermotoga maritima tRNAGln(CUG) in vitro transcript, wild type |