Values CatLog read from this paper
Kinetic properties of wild-type and altered recombinant amidases by the use of ion-selective electrode assay method
PMID 16792995doi:10.1016/j.ab.2006.04.023Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 44 records (132 values) from this paper: amidase (aliphatic amidase, acylamide amidohydrolase) from Pseudomonas aeruginosa.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 5.73 | 1.33 | 4.4 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide10 mM hydroxylamine; Michaelis-Menten plot fit | 13.74 | 1.14 | 12.1 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide30 mM hydroxylamine; Michaelis-Menten plot fit | 21.83 | 1.72 | 12.7 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 4.04 | 0.71 | 5.7 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide10 mM hydroxylamine; Lineweaver-Burk plot fit | 14.02 | 1.19 | 11.8 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide30 mM hydroxylamine; Lineweaver-Burk plot fit | 15.56 | 1.02 | 15.3 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamide20 mM potassium phosphate buffer; acrylamide varied 0-100 mM | 59.4 | 3.3 | 18.1 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; 3 ml stirred | 64.04 | 11.2 | 5.7 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamide20 mM potassium phosphate buffer; acrylamide varied 0-100 mM | 44.78 | 2.7 | 16.5 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; 3 ml stirred | 61.18 | 10.1 | 6.1 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 77.29 | 9.72 | 7.95 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 74.75 | 10.4 | 7.21 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 74.76 | 8.62 | 8.76 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 71.4 | 9.77 | 7.33 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 0.81 | 1.69 | 0.491 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Michaelis-Menten plot fit | 2.48 | 3.81 | 0.651 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide2 mM hydroxylamine; Michaelis-Menten plot fit | 1.79 | 2.33 | 0.765 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 0.65 | 1.23 | 0.527 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Lineweaver-Burk plot fit | 1.66 | 2.59 | 0.642 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide2 mM hydroxylamine; Lineweaver-Burk plot fit | 1.37 | 1.83 | 0.752 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 12.35 | 0.364 | 34 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide5 mM hydroxylamine; Michaelis-Menten plot fit | 20.14 | 0.479 | 42.3 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Michaelis-Menten plot fit | 30.68 | 0.872 | 35.2 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 11.85 | 0.354 | 33.8 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide5 mM hydroxylamine; Lineweaver-Burk plot fit | 19.06 | 0.495 | 39.5 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Lineweaver-Burk plot fit | 31.04 | 0.937 | 33.3 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 2.9 | 0.3 | 10 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Michaelis-Menten plot fit | 11.45 | 1 | 11.6 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide200 mM hydroxylamine; Michaelis-Menten plot fit | 30.52 | 18 | 1.7 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 2.3 | 0.23 | 10.3 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Lineweaver-Burk plot fit | 12.97 | 1.19 | 11.2 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide200 mM hydroxylamine; Lineweaver-Burk plot fit | 29.12 | 15.9 | 1.9 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 0.81 | 9.5 | 0.086 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide5 mM hydroxylamine; Michaelis-Menten plot fit | 1.1 | 7.3 | 0.165 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide20 mM hydroxylamine; Michaelis-Menten plot fit | 1.46 | 12.1 | 0.122 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 0.77 | 9 | 0.086 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide5 mM hydroxylamine; Lineweaver-Burk plot fit | 1.18 | 10.4 | 0.114 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide20 mM hydroxylamine; Lineweaver-Burk plot fit | 1.67 | 17.6 | 0.096 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 8.18 | 0.56 | 14.8 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Michaelis-Menten plot fit | 22.84 | 1.16 | 19.8 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide10 mM hydroxylamine; Michaelis-Menten plot fit | 45.71 | 2.18 | 21 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 8.96 | 0.6 | 15.2 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Lineweaver-Burk plot fit | 21.98 | 1.11 | 19.9 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide10 mM hydroxylamine; Lineweaver-Burk plot fit | 53.98 | 2.6 | 20.7 | pH 7.2, 25 °C | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |