EC 3.5.1.4: amidase
HydrolasesOpen in Browse
CatLog read 145 records for EC 3.5.1.4 from 6 papers, for 4 organisms. Names in these papers: enantioselective amidase, amidase (aliphatic amidase, acylamide amidohydrolase), signature amidase (SSAM), (+)-γ-lactamase Sslact and 2 more.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 59 records for EC 3.5.1.4 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 3.5.1.4 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Acyl transfer activity of an amidase from Rhodococcus sp. strain R312: formation of a wide range of hydroxamic acids | Rhodococcus sp. | 56 |
| Kinetic properties of wild-type and altered recombinant amidases by the use of ion-selective electrode assay method | Pseudomonas aeruginosa | 44 |
| Oligomerization of Sulfolobus solfataricus signature amidase is promoted by acidic pH and high temperature | Sulfolobus solfataricus | 30 |
| Enhancement in the catalytic activity of Sulfolobus solfataricus P2 (+)-γ-lactamase by semi-rational design with the aid of a newly established high-throughput screening method | Sulfolobus solfataricus | 6 |
| Nitrilase superfamily aryl acylamidase from the halotolerant mangrove Streptomyces sp. 211726 | Streptomyces sp. 211726 | 5 |
| Structure and characterization of amidase from Rhodococcus sp. N-771: Insight into the molecular mechanism of substrate recognition | Rhodococcus sp. | 4 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| (+)-γ-lactamase Sslact | Sulfolobus solfataricus | 2-azabicyclo[2.2.1]hept-5-en-3-one | 1.41 | 61.3 | 0.023 | PMID 30310965 | Open record: (+)-γ-lactamase Sslact, 2-azabicyclo[2.2.1]hept-5-en-3-one | |
| (+)-γ-lactamase Sslactvariant I336R | Sulfolobus solfataricus | 2-azabicyclo[2.2.1]hept-5-en-3-one | 4.76 | 55.9 | 0.08515 | PMID 30310965 | Open record: (+)-γ-lactamase Sslact variant I336R, 2-azabicyclo[2.2.1]hept-5-en-3-one | |
| (+)-γ-lactamase Sslactvariant V203N/I336R | Sulfolobus solfataricus | 2-azabicyclo[2.2.1]hept-5-en-3-one | 8.06 | 26.5 | 0.3041 | PMID 30310965 | Open record: (+)-γ-lactamase Sslact variant V203N/I336R, 2-azabicyclo[2.2.1]hept-5-en-3-one | |
| (+)-γ-lactamase Sslactvariant V203N/Y388H | Sulfolobus solfataricus | 2-azabicyclo[2.2.1]hept-5-en-3-one | 1.54 | 22 | 0.07 | PMID 30310965 | Open record: (+)-γ-lactamase Sslact variant V203N/Y388H, 2-azabicyclo[2.2.1]hept-5-en-3-one | |
| (+)-γ-lactamase Sslactvariant V203N/Y388H/I336R | Sulfolobus solfataricus | 2-azabicyclo[2.2.1]hept-5-en-3-one | 9.04 | 17.5 | 0.5166 | PMID 30310965 | Open record: (+)-γ-lactamase Sslact variant V203N/Y388H/I336R, 2-azabicyclo[2.2.1]hept-5-en-3-one | |
| (+)-γ-lactamase Sslactvariant Y388H/I336R | Sulfolobus solfataricus | 2-azabicyclo[2.2.1]hept-5-en-3-one | 7.69 | 60.8 | 0.1265 | PMID 30310965 | Open record: (+)-γ-lactamase Sslact variant Y388H/I336R, 2-azabicyclo[2.2.1]hept-5-en-3-one | |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 5.73 | 1.33 | 4.4 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide10 mM hydroxylamine; Michaelis-Menten plot fit | 13.74 | 1.14 | 12.1 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide30 mM hydroxylamine; Michaelis-Menten plot fit | 21.83 | 1.72 | 12.7 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 4.04 | 0.71 | 5.7 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide10 mM hydroxylamine; Lineweaver-Burk plot fit | 14.02 | 1.19 | 11.8 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acetamide30 mM hydroxylamine; Lineweaver-Burk plot fit | 15.56 | 1.02 | 15.3 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamide20 mM potassium phosphate buffer; acrylamide varied 0-100 mM | 59.4 | 3.3 | 18.1 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; 3 ml stirred | 64.04 | 11.2 | 5.7 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamide20 mM potassium phosphate buffer; acrylamide varied 0-100 mM | 44.78 | 2.7 | 16.5 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; 3 ml stirred | 61.18 | 10.1 | 6.1 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 77.29 | 9.72 | 7.95 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 74.75 | 10.4 | 7.21 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 74.76 | 8.62 | 8.76 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | acrylamideISE method: ammonium ion-selective electrode; Tris buffer | 71.4 | 9.77 | 7.33 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 0.81 | 1.69 | 0.491 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Michaelis-Menten plot fit | 2.48 | 3.81 | 0.651 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide2 mM hydroxylamine; Michaelis-Menten plot fit | 1.79 | 2.33 | 0.765 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 0.65 | 1.23 | 0.527 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Lineweaver-Burk plot fit | 1.66 | 2.59 | 0.642 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase) | Pseudomonas aeruginosa | p-nitroacetanilide2 mM hydroxylamine; Lineweaver-Burk plot fit | 1.37 | 1.83 | 0.752 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase), p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 12.35 | 0.364 | 34 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide5 mM hydroxylamine; Michaelis-Menten plot fit | 20.14 | 0.479 | 42.3 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Michaelis-Menten plot fit | 30.68 | 0.872 | 35.2 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 11.85 | 0.354 | 33.8 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide5 mM hydroxylamine; Lineweaver-Burk plot fit | 19.06 | 0.495 | 39.5 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant C91A | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Lineweaver-Burk plot fit | 31.04 | 0.937 | 33.3 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant C91A, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 2.9 | 0.3 | 10 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Michaelis-Menten plot fit | 11.45 | 1 | 11.6 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide200 mM hydroxylamine; Michaelis-Menten plot fit | 30.52 | 18 | 1.7 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 2.3 | 0.23 | 10.3 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide20 mM hydroxylamine; Lineweaver-Burk plot fit | 12.97 | 1.19 | 11.2 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acetamide200 mM hydroxylamine; Lineweaver-Burk plot fit | 29.12 | 15.9 | 1.9 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acetamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 0.81 | 9.5 | 0.086 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide5 mM hydroxylamine; Michaelis-Menten plot fit | 1.1 | 7.3 | 0.165 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide20 mM hydroxylamine; Michaelis-Menten plot fit | 1.46 | 12.1 | 0.122 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 0.77 | 9 | 0.086 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide5 mM hydroxylamine; Lineweaver-Burk plot fit | 1.18 | 10.4 | 0.114 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | acrylamide20 mM hydroxylamine; Lineweaver-Burk plot fit | 1.67 | 17.6 | 0.096 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, acrylamide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Michaelis-Menten plot fit | 8.18 | 0.56 | 14.8 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Michaelis-Menten plot fit | 22.84 | 1.16 | 19.8 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide10 mM hydroxylamine; Michaelis-Menten plot fit | 45.71 | 2.18 | 21 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilideno hydroxylamine (0 mM); Lineweaver-Burk plot fit | 8.96 | 0.6 | 15.2 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide0.5 mM hydroxylamine; Lineweaver-Burk plot fit | 21.98 | 1.11 | 19.9 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (aliphatic amidase, acylamide amidohydrolase)variant T103I | Pseudomonas aeruginosa | p-nitroacetanilide10 mM hydroxylamine; Lineweaver-Burk plot fit | 53.98 | 2.6 | 20.7 | pH 7.2, 25 °C | PMID 16792995 | Open record: amidase (aliphatic amidase, acylamide amidohydrolase) variant T103I, p-nitroacetanilide |
| amidase (RhAmidase) | Rhodococcus sp. | acetamide | 20.2 | 19 | 1.14 | pH 5.9, 55 °C | PMID 19819352 | Open record: amidase (RhAmidase), acetamide |
| amidase (RhAmidase) | Rhodococcus sp. | benzamide | 157.7 | 1.03 | 153.5 | pH 5.9, 55 °C | PMID 19819352 | Open record: amidase (RhAmidase), benzamide |
| amidase (RhAmidase) | Rhodococcus sp. | prop-2-enamide | 2.1 | 24.1 | 0.087 | pH 5.9, 55 °C | PMID 19819352 | Open record: amidase (RhAmidase), prop-2-enamide |
| amidase (RhAmidase) | Rhodococcus sp. | propanamide | 155.3 | 34.2 | 4.54 | pH 5.9, 55 °C | PMID 19819352 | Open record: amidase (RhAmidase), propanamide |
| Azl13 (nitrilase superfamily amidase / aryl acylamidase) | Streptomyces sp. 211726 | 4-guanidinobutanamide | 55.6 | 2.3 | 24.17 | pH 8, 35 °C | PMID 24752846 | Open record: Azl13 (nitrilase superfamily amidase / aryl acylamidase), 4-guanidinobutanamide |
| Azl13 (nitrilase superfamily amidase / aryl acylamidase) | Streptomyces sp. 211726 | Acetamide | 29.9 | 39.4 | 0.76 | pH 8, 35 °C | PMID 24752846 | Open record: Azl13 (nitrilase superfamily amidase / aryl acylamidase), Acetamide |
| Azl13 (nitrilase superfamily amidase / aryl acylamidase) | Streptomyces sp. 211726 | Benzamide | — | — | 0.034 | pH 8, 35 °C | PMID 24752846 | Open record: Azl13 (nitrilase superfamily amidase / aryl acylamidase), Benzamide |
| Azl13 (nitrilase superfamily amidase / aryl acylamidase) | Streptomyces sp. 211726 | Nicotinamide | 5.6 | 135.6 | 0.042 | pH 8, 35 °C | PMID 24752846 | Open record: Azl13 (nitrilase superfamily amidase / aryl acylamidase), Nicotinamide |
| Azl13 (nitrilase superfamily amidase / aryl acylamidase) | Streptomyces sp. 211726 | Propionamide | 28.6 | 45.1 | 0.64 | pH 8, 35 °C | PMID 24752846 | Open record: Azl13 (nitrilase superfamily amidase / aryl acylamidase), Propionamide |
| enantioselective amidase | Rhodococcus sp. | Acetamide | 79 | 70 | 1.1 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Acetamide |
| enantioselective amidase | Rhodococcus sp. | Acrylamide | 16 | 3 | 5.3 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Acrylamide |
| enantioselective amidase | Rhodococcus sp. | Benzamide | 6 | 0.1 | 60 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Benzamide |
| enantioselective amidase | Rhodococcus sp. | Benzamide | 6.2 | — | — | 30 °C | PMID 9687439 | Open record: enantioselective amidase, Benzamide |
| enantioselective amidase | Rhodococcus sp. | benzohydroxamic acid | 4.3 | — | — | 30 °C | PMID 9687439 | Open record: enantioselective amidase, benzohydroxamic acid |
| enantioselective amidase | Rhodococcus sp. | Butyramide | 229 | 0.1 | 2.3 × 10³ calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Butyramide |
| enantioselective amidase | Rhodococcus sp. | D-Alaninamide | 3353 | 379 | 8.85 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, D-Alaninamide |
| enantioselective amidase | Rhodococcus sp. | D-Lactamide | 2504 | 270 | 9.3 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, D-Lactamide |
| enantioselective amidase | Rhodococcus sp. | DL-Lactamide | 426 | 51 | 8.4 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, DL-Lactamide |
| enantioselective amidase | Rhodococcus sp. | DL-Leucinamide | 24 | 2.4 | 10 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, DL-Leucinamide |
| enantioselective amidase | Rhodococcus sp. | DL-Methioninamide | 180 | 6.4 | 28 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, DL-Methioninamide |
| enantioselective amidase | Rhodococcus sp. | DL-Phenylalaninamide | 6.2 | 1.1 | 5.6 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, DL-Phenylalaninamide |
| enantioselective amidase | Rhodococcus sp. | Hexanoamide | 333 | 0.3 | 1.1 × 10³ calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Hexanoamide |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Acetamide | — | 20 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Propionamide | — | 82 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Butyramide | — | 178 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Isobutyramide | — | 134 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Valeramide | — | 230 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Pivalamide | — | 55 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Hexanoamide | — | 536 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Acrylamide | — | 5 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Methacrylamide | — | 7 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Benzamide | — | 14 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Nicotinamide | — | 13 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Isonicotinamide | — | 62 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Lactamide | — | 41 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Alaninamide | — | 25 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Leucinamide | — | 39 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Methioninamide | — | 36 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Phenylalaninamide | — | 19 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Prolinamide | — | 206 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Threoninamide | — | 25 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Methioninamide | — | 75 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Leucinamide | — | 187 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Phenylalaninamide | — | 19 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Lactamide | — | 507 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate D-Lactamide | — | 2923 | — | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate D-Alaninamide | — | 1939 | — | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | Isobutyramide | 109 | 0.1 | 1.1 × 10³ calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Isobutyramide |
| enantioselective amidase | Rhodococcus sp. | Isonicotinamide | 2 | 0.6 | 3.3 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Isonicotinamide |
| enantioselective amidase | Rhodococcus sp. | L-Alaninamide | 66 | 12 | 5.5 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Alaninamide |
| enantioselective amidase | Rhodococcus sp. | L-Lactamide | 67 | 33 | 2.0 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Lactamide |
| enantioselective amidase | Rhodococcus sp. | L-Leucinamide | 63 | 1.3 | 48 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Leucinamide |
| enantioselective amidase | Rhodococcus sp. | L-leucine hydroxamic acid | — | 4.9 | — | 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-leucine hydroxamic acid |
| enantioselective amidase | Rhodococcus sp. | L-Methioninamide | 245 | 5.4 | 45 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Methioninamide |
| enantioselective amidase | Rhodococcus sp. | L-Phenylalaninamide | 8.1 | 0.8 | 10 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Phenylalaninamide |
| enantioselective amidase | Rhodococcus sp. | L-Prolinamide | 48 | 2 | 24 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Prolinamide |
| enantioselective amidase | Rhodococcus sp. | L-Threoninamide | 38 | 16 | 2.4 calc. | pH 8, 30 °C | PMID 9687439 | Open record: enantioselective amidase, L-Threoninamide |
| enantioselective amidase | Rhodococcus sp. | Methacrylamide | 8 | 0.1 | 80 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Methacrylamide |
| enantioselective amidase | Rhodococcus sp. | Nicotinamide | 8 | 0.3 | 27 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Nicotinamide |
| enantioselective amidase | Rhodococcus sp. | Pivalamide | 15 | 0.1 | 150 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Pivalamide |
| enantioselective amidase | Rhodococcus sp. | Propionamide | 192 | 1 | 190 calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Propionamide |
| enantioselective amidase | Rhodococcus sp. | Valeramide | 313 | 0.1 | 3.1 × 10³ calc. | pH 7, 30 °C | PMID 9687439 | Open record: enantioselective amidase, Valeramide |
| enantioselective amidase | Rhodococcus sp. | Valeramide | 35 | — | — | 30 °C | PMID 9687439 | Open record: enantioselective amidase, Valeramide |
| enantioselective amidase | Rhodococcus sp. | valerohydroxamic acidhydroxylamine presence and pH not stated for these values | 18 | — | — | 30 °C | PMID 9687439 | Open record: enantioselective amidase, valerohydroxamic acid |
| enantioselective amidase | Rhodococcus sp. | valerohydroxamic acid | — | 0.2 | — | 30 °C | PMID 9687439 | Open record: enantioselective amidase, valerohydroxamic acid |
| signature amidase (SSAM) | Sulfolobus solfataricus | 2-methylbenzamide | 0.4 | 0.1 | 4 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), 2-methylbenzamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | 2-methylpropanamide | 48.9 | 7 | 6.99 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), 2-methylpropanamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | 3-phenylpropanamide | 15.3 | 3 | 5.1 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), 3-phenylpropanamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | 4-aminobenzamide | 0.8 | 0.2 | 4 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), 4-aminobenzamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | 4-hydroxybenzamide | 1.9 | 0.1 | 19 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), 4-hydroxybenzamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | 4-methylbenzamide | 12.2 | 0.2 | 61 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), 4-methylbenzamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | Acetamide | 28.9 | 22 | 1.31 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), Acetamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | Benzamide | 6.7 | 0.9 | 7.44 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), Benzamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | butanamide | 34.3 | 14 | 2.45 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), butanamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | Hexanamide | 19.5 | 4.5 | 4.33 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), Hexanamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | indole-3-acetamide (2-(1H-indol-3-yl)acetamide) | 2.2 | 0.8 | 2.75 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), indole-3-acetamide (2-(1H-indol-3-yl)acetamide) |
| signature amidase (SSAM) | Sulfolobus solfataricus | methacrylamide (2-methylprop-2-enamide) | 4.8 | 0.3 | 16 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), methacrylamide (2-methylprop-2-enamide) |
| signature amidase (SSAM) | Sulfolobus solfataricus | Nicotinamide | 6.4 | 1.5 | 4.26 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), Nicotinamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | Pentanamide | 26.8 | 2.5 | 10.72 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), Pentanamide |
| signature amidase (SSAM) | Sulfolobus solfataricus | propanamide | 82.9 | 10 | 8.29 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM), propanamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | 2-methylbenzamide | 0.8 | 0.2 | 4 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, 2-methylbenzamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | 2-methylpropanamide | 58.4 | 6.5 | 8.98 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, 2-methylpropanamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | 3-phenylpropanamide | 18 | 2.5 | 7.2 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, 3-phenylpropanamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | 4-aminobenzamide | 2.5 | 0.3 | 8.33 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, 4-aminobenzamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | 4-hydroxybenzamide | 2.1 | 0.1 | 21 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, 4-hydroxybenzamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | 4-methylbenzamide | 14.2 | 0.3 | 47.33 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, 4-methylbenzamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | Acetamide | 30.1 | 37 | 0.81 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, Acetamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | Benzamide | 7.2 | 0.6 | 12 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, Benzamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | butanamide | 17.5 | 3.2 | 5.47 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, butanamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | Hexanamide | 21.6 | 4.5 | 4.8 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, Hexanamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | indole-3-acetamide (2-(1H-indol-3-yl)acetamide) | 1.2 | 0.8 | 1.5 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, indole-3-acetamide (2-(1H-indol-3-yl)acetamide) |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | methacrylamide (2-methylprop-2-enamide) | 3 | 0.5 | 6 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, methacrylamide (2-methylprop-2-enamide) |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | Nicotinamide | 2.2 | 0.4 | 5.5 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, Nicotinamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | Pentanamide | 28.6 | 1.5 | 19.06 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, Pentanamide |
| signature amidase (SSAM)variant Y41C | Sulfolobus solfataricus | propanamide | 99.4 | 10 | 9.94 | pH 5, 70 °C | PMID 16243781 | Open record: signature amidase (SSAM) variant Y41C, propanamide |