Values CatLog read from this paper
Acyl transfer activity of an amidase from Rhodococcus sp. strain R312: formation of a wide range of hydroxamic acids
PMID 9687439doi:10.1128/aem.64.8.2844-2852.1998Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 56 records (81 values) from this paper: enantioselective amidase from Rhodococcus sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 25 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| enantioselective amidase | Rhodococcus sp. | Acetamide | 79 | 70 | 1.1 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Acetamide |
| enantioselective amidase | Rhodococcus sp. | Acrylamide | 16 | 3 | 5.3 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Acrylamide |
| enantioselective amidase | Rhodococcus sp. | Benzamide | 6 | 0.1 | 60 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Benzamide |
| enantioselective amidase | Rhodococcus sp. | Benzamide | 6.2 | — | — | 30 °C | Open record: enantioselective amidase, Benzamide |
| enantioselective amidase | Rhodococcus sp. | benzohydroxamic acid | 4.3 | — | — | 30 °C | Open record: enantioselective amidase, benzohydroxamic acid |
| enantioselective amidase | Rhodococcus sp. | Butyramide | 229 | 0.1 | 2.3 × 10³ calc. | pH 7, 30 °C | Open record: enantioselective amidase, Butyramide |
| enantioselective amidase | Rhodococcus sp. | D-Alaninamide | 3353 | 379 | 8.85 calc. | pH 8, 30 °C | Open record: enantioselective amidase, D-Alaninamide |
| enantioselective amidase | Rhodococcus sp. | D-Lactamide | 2504 | 270 | 9.3 calc. | pH 7, 30 °C | Open record: enantioselective amidase, D-Lactamide |
| enantioselective amidase | Rhodococcus sp. | DL-Lactamide | 426 | 51 | 8.4 calc. | pH 7, 30 °C | Open record: enantioselective amidase, DL-Lactamide |
| enantioselective amidase | Rhodococcus sp. | DL-Leucinamide | 24 | 2.4 | 10 calc. | pH 8, 30 °C | Open record: enantioselective amidase, DL-Leucinamide |
| enantioselective amidase | Rhodococcus sp. | DL-Methioninamide | 180 | 6.4 | 28 calc. | pH 8, 30 °C | Open record: enantioselective amidase, DL-Methioninamide |
| enantioselective amidase | Rhodococcus sp. | DL-Phenylalaninamide | 6.2 | 1.1 | 5.6 calc. | pH 8, 30 °C | Open record: enantioselective amidase, DL-Phenylalaninamide |
| enantioselective amidase | Rhodococcus sp. | Hexanoamide | 333 | 0.3 | 1.1 × 10³ calc. | pH 7, 30 °C | Open record: enantioselective amidase, Hexanoamide |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Acetamide | — | 20 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Propionamide | — | 82 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Butyramide | — | 178 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Isobutyramide | — | 134 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Valeramide | — | 230 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Pivalamide | — | 55 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Hexanoamide | — | 536 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Acrylamide | — | 5 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Methacrylamide | — | 7 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Benzamide | — | 14 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Nicotinamide | — | 13 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate Isonicotinamide | — | 62 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Lactamide | — | 41 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Alaninamide | — | 25 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Leucinamide | — | 39 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Methioninamide | — | 36 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Phenylalaninamide | — | 19 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Prolinamide | — | 206 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate L-Threoninamide | — | 25 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Methioninamide | — | 75 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Leucinamide | — | 187 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Phenylalaninamide | — | 19 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate DL-Lactamide | — | 507 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate D-Lactamide | — | 2923 | — | pH 7, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | hydroxylaminecosubstrate D-Alaninamide | — | 1939 | — | pH 8, 30 °C | Open record: enantioselective amidase, hydroxylamine |
| enantioselective amidase | Rhodococcus sp. | Isobutyramide | 109 | 0.1 | 1.1 × 10³ calc. | pH 7, 30 °C | Open record: enantioselective amidase, Isobutyramide |
| enantioselective amidase | Rhodococcus sp. | Isonicotinamide | 2 | 0.6 | 3.3 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Isonicotinamide |
| enantioselective amidase | Rhodococcus sp. | L-Alaninamide | 66 | 12 | 5.5 calc. | pH 8, 30 °C | Open record: enantioselective amidase, L-Alaninamide |
| enantioselective amidase | Rhodococcus sp. | L-Lactamide | 67 | 33 | 2.0 calc. | pH 7, 30 °C | Open record: enantioselective amidase, L-Lactamide |
| enantioselective amidase | Rhodococcus sp. | L-Leucinamide | 63 | 1.3 | 48 calc. | pH 8, 30 °C | Open record: enantioselective amidase, L-Leucinamide |
| enantioselective amidase | Rhodococcus sp. | L-leucine hydroxamic acid | — | 4.9 | — | 30 °C | Open record: enantioselective amidase, L-leucine hydroxamic acid |
| enantioselective amidase | Rhodococcus sp. | L-Methioninamide | 245 | 5.4 | 45 calc. | pH 8, 30 °C | Open record: enantioselective amidase, L-Methioninamide |
| enantioselective amidase | Rhodococcus sp. | L-Phenylalaninamide | 8.1 | 0.8 | 10 calc. | pH 8, 30 °C | Open record: enantioselective amidase, L-Phenylalaninamide |
| enantioselective amidase | Rhodococcus sp. | L-Prolinamide | 48 | 2 | 24 calc. | pH 8, 30 °C | Open record: enantioselective amidase, L-Prolinamide |
| enantioselective amidase | Rhodococcus sp. | L-Threoninamide | 38 | 16 | 2.4 calc. | pH 8, 30 °C | Open record: enantioselective amidase, L-Threoninamide |
| enantioselective amidase | Rhodococcus sp. | Methacrylamide | 8 | 0.1 | 80 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Methacrylamide |
| enantioselective amidase | Rhodococcus sp. | Nicotinamide | 8 | 0.3 | 27 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Nicotinamide |
| enantioselective amidase | Rhodococcus sp. | Pivalamide | 15 | 0.1 | 150 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Pivalamide |
| enantioselective amidase | Rhodococcus sp. | Propionamide | 192 | 1 | 190 calc. | pH 7, 30 °C | Open record: enantioselective amidase, Propionamide |
| enantioselective amidase | Rhodococcus sp. | Valeramide | 313 | 0.1 | 3.1 × 10³ calc. | pH 7, 30 °C | Open record: enantioselective amidase, Valeramide |
| enantioselective amidase | Rhodococcus sp. | Valeramide | 35 | — | — | 30 °C | Open record: enantioselective amidase, Valeramide |
| enantioselective amidase | Rhodococcus sp. | valerohydroxamic acidhydroxylamine presence and pH not stated for these values | 18 | — | — | 30 °C | Open record: enantioselective amidase, valerohydroxamic acid |
| enantioselective amidase | Rhodococcus sp. | valerohydroxamic acid | — | 0.2 | — | 30 °C | Open record: enantioselective amidase, valerohydroxamic acid |