Values CatLog read from this paper

Aspartic acid 214 in Citrobacter freundii tyrosine phenol-lyase ensures sufficient C--H-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site

PMID 16793353doi:10.1016/j.bbapap.2006.05.001Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 17 records (33 values) from this paper: tyrosine phenol-lyase from Citrobacter freundii.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 4.1.99.2

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	3-fluoro-L-tyrosine	0.038	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214A, 3-fluoro-L-tyrosine
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	L-aspartic acid	0.25	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214A, L-aspartic acid
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	L-glutamic acid	0.148	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214A, L-glutamic acid
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	L-methionine	0.076	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214A, L-methionine
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	L-phenylalanine	0.0096	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214A, L-phenylalanine
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	S-(o-nitrophenyl)-L-cysteine	0.024	1.32	0.018	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214A, S-(o-nitrophenyl)-L-cysteine
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	S-benzyl-L-cysteine	0.02	7	0.00285	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214A, S-benzyl-L-cysteine
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	S-methyl-L-cysteine	0.0052	50	1.04 × 10⁻⁴	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214A, S-methyl-L-cysteine
tyrosine phenol-lyasevariant D214A	Citrobacter freundii	β-chloro-L-alanine	0.02	13.3	0.0015	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214A, β-chloro-L-alanine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	3-fluoro-L-tyrosine	0.022	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214N, 3-fluoro-L-tyrosine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	L-aspartic acid	0.038	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214N, L-aspartic acid
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	L-methionine	0.13	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214N, L-methionine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	L-phenylalanine	0.018	—	—	pH 8.2	Open record: tyrosine phenol-lyase variant D214N, L-phenylalanine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	S-(o-nitrophenyl)-L-cysteine	0.041	1.86	0.022	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214N, S-(o-nitrophenyl)-L-cysteine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	S-benzyl-L-cysteine	0.0067	5.1	0.0013	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214N, S-benzyl-L-cysteine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	S-methyl-L-cysteine	0.0016	13.4	1.2 × 10⁻⁴	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214N, S-methyl-L-cysteine
tyrosine phenol-lyasevariant D214N	Citrobacter freundii	β-chloro-L-alanine	0.0065	11.4	5.7 × 10⁻⁴	pH 8, 25 °C	Open record: tyrosine phenol-lyase variant D214N, β-chloro-L-alanine