EC 4.1.99.2: tyrosine phenol-lyase
LyasesOpen in Browse
CatLog read 60 records for EC 4.1.99.2 from 6 papers, for 1 organism. Names in these papers: tyrosine phenol-lyase, tyrosine phenol-lyase (TPL), tyrosine phenol lyase (CfTPL).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units.
CatLog also holds 150 records for EC 4.1.99.2 that are not yet re-read: from its earlier version, built from the enzyme databases and partly checked against the papers. All records for EC 4.1.99.2 in Browse.
Papers
| Paper | Organisms | Records |
|---|---|---|
| Aspartic acid 214 in Citrobacter freundii tyrosine phenol-lyase ensures sufficient C--H-acidity of the external aldimine intermediate and proper orientation of the cofactor at the active site | Citrobacter freundii | 17 |
| Serine 51 residue of Citrobacter freundii tyrosine phenol-lyase assists in C-α-proton abstraction and transfer in the reaction with substrate | Citrobacter freundii | 12 |
| M379A Mutant Tyrosine Phenol-lyase from Citrobacter freundii Has Altered Conformational Dynamics | Citrobacter freundii | 11 |
| doi:10.1021/acscatal.6b01495 | Citrobacter freundii | 9 |
| Phage-assisted continuous evolution of enzymes for noncanonical tyrosine biosynthesis | Citrobacter freundii | 6 |
| Hidden Activities of Tyrosine Phenol-Lyase and Tryptophan Indole-Lyase: Recombinant PLP-Dependent C-C Lyases as New Biocatalysts for Antimicrobial Thiosulfinate Generation | Citrobacter freundii | 5 |
Records
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Paper | Open record |
|---|---|---|---|---|---|---|---|---|
| tyrosine phenol lyase (CfTPL)variant M379V | Citrobacter freundii | 3-iodotyrosine | 0.042 | 0.267 | 0.156 | pH 8, 37 °C | PMID 42721086 | Open record: tyrosine phenol lyase (CfTPL) variant M379V, 3-iodotyrosine |
| tyrosine phenol lyase (CfTPL)variant M379V | Citrobacter freundii | L-tyrosine | 0.25 | 0.814 | 0.307 | pH 8, 37 °C | PMID 42721086 | Open record: tyrosine phenol lyase (CfTPL) variant M379V, L-tyrosine |
| tyrosine phenol lyase (CfTPL)variant M379V/C179S (N1.2-4) | Citrobacter freundii | 3-iodotyrosine | 0.042 | 0.248 | 0.169 | pH 8, 37 °C | PMID 42721086 | Open record: tyrosine phenol lyase (CfTPL) variant M379V/C179S (N1.2-4), 3-iodotyrosine |
| tyrosine phenol lyase (CfTPL)variant M379V/C179S (N1.2-4) | Citrobacter freundii | L-tyrosine | 0.283 | 0.767 | 0.37 | pH 8, 37 °C | PMID 42721086 | Open record: tyrosine phenol lyase (CfTPL) variant M379V/C179S (N1.2-4), L-tyrosine |
| tyrosine phenol lyase (CfTPL)variant M379V/V16I/V141I/C179S/N390H (N2.2-1) | Citrobacter freundii | 3-iodotyrosine | 0.057 | 0.392 | 0.146 | pH 8, 37 °C | PMID 42721086 | Open record: tyrosine phenol lyase (CfTPL) variant M379V/V16I/V141I/C179S/N390H (N2.2-1), 3-iodotyrosine |
| tyrosine phenol lyase (CfTPL)variant M379V/V16I/V141I/C179S/N390H (N2.2-1) | Citrobacter freundii | L-tyrosine | 0.19 | 1.048 | 0.182 | pH 8, 37 °C | PMID 42721086 | Open record: tyrosine phenol lyase (CfTPL) variant M379V/V16I/V141I/C179S/N390H (N2.2-1), L-tyrosine |
| tyrosine phenol-lyase | Citrobacter freundii | 3-chloro-L-alanine | 6 | 2.12 | 2.84 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase, 3-chloro-L-alanine |
| tyrosine phenol-lyase | Citrobacter freundii | S-(2-nitrophenyl)-L-cysteine | 9.65 | 0.21 | 46 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase, S-(2-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyase | Citrobacter freundii | S-benzyl-L-cysteine | 0.63 | 0.12 | 5.26 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase, S-benzyl-L-cysteine |
| tyrosine phenol-lyase | Citrobacter freundii | S-ethyl-L-cysteine | 5.4 | 1.57 | 3.43 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase, S-ethyl-L-cysteine |
| tyrosine phenol-lyase | Citrobacter freundii | S-methyl-L-cysteine | 1.13 | 2.32 | 0.49 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase, S-methyl-L-cysteine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | 3-fluoro-L-tyrosine | 0.038 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, 3-fluoro-L-tyrosine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | L-aspartic acid | 0.25 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, L-aspartic acid |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | L-glutamic acid | 0.148 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, L-glutamic acid |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | L-methionine | 0.076 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, L-methionine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | L-phenylalanine | 0.0096 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, L-phenylalanine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | S-(o-nitrophenyl)-L-cysteine | 0.024 | 1.32 | 0.018 | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, S-(o-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | S-benzyl-L-cysteine | 0.02 | 7 | 0.00285 | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, S-benzyl-L-cysteine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | S-methyl-L-cysteine | 0.0052 | 50 | 1.04 × 10⁻⁴ | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, S-methyl-L-cysteine |
| tyrosine phenol-lyasevariant D214A | Citrobacter freundii | β-chloro-L-alanine | 0.02 | 13.3 | 0.0015 | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214A, β-chloro-L-alanine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | 3-fluoro-L-tyrosine | 0.022 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, 3-fluoro-L-tyrosine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | L-aspartic acid | 0.038 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, L-aspartic acid |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | L-methionine | 0.13 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, L-methionine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | L-phenylalanine | 0.018 | — | — | pH 8.2 | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, L-phenylalanine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | S-(o-nitrophenyl)-L-cysteine | 0.041 | 1.86 | 0.022 | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, S-(o-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | S-benzyl-L-cysteine | 0.0067 | 5.1 | 0.0013 | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, S-benzyl-L-cysteine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | S-methyl-L-cysteine | 0.0016 | 13.4 | 1.2 × 10⁻⁴ | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, S-methyl-L-cysteine |
| tyrosine phenol-lyasevariant D214N | Citrobacter freundii | β-chloro-L-alanine | 0.0065 | 11.4 | 5.7 × 10⁻⁴ | pH 8, 25 °C | PMID 16793353 | Open record: tyrosine phenol-lyase variant D214N, β-chloro-L-alanine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | 3-chloro-L-alanine | 0.15 | 0.8 | 0.19 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, 3-chloro-L-alanine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | 3-fluoro-L-tyrosine | 0.012 | 1.3 | 0.0092 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, 3-fluoro-L-tyrosine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | L-tyrosine | 0.03 | 1.3 | 0.022 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, L-tyrosine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | S-(2-nitrophenyl)-L-cysteine | 8.7 | 2.2 | 3.9 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, S-(2-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | S-benzyl-L-cysteine | 0.015 | 4.1 | 0.0037 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, S-benzyl-L-cysteine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | S-ethyl-L-cysteine | 0.083 | 5.4 | 0.015 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, S-ethyl-L-cysteine |
| tyrosine phenol-lyasevariant S51A | Citrobacter freundii | S-methyl-L-cysteine | 0.044 | 10.5 | 0.0042 | pH 8, 30 °C | PMID 29183854 | Open record: tyrosine phenol-lyase variant S51A, S-methyl-L-cysteine |
| tyrosine phenol-lyase (TPL) | Citrobacter freundii | L-3,4-dihydroxyphenylalanine | — | — | 0.0272 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL), L-3,4-dihydroxyphenylalanine | |
| tyrosine phenol-lyase (TPL) | Citrobacter freundii | S-allyl-L-cysteine sulfoxide | 15.4 | 3.42 | 4.5 | pH 8, 37 °C | PMID 41754831 | Open record: tyrosine phenol-lyase (TPL), S-allyl-L-cysteine sulfoxide |
| tyrosine phenol-lyase (TPL) | Citrobacter freundii | S-benzyl-L-cysteine sulfoxide | 19 | 2.4 | 7.9 | pH 8, 37 °C | PMID 41754831 | Open record: tyrosine phenol-lyase (TPL), S-benzyl-L-cysteine sulfoxide |
| tyrosine phenol-lyase (TPL) | Citrobacter freundii | S-ethyl-L-cysteine sulfoxide | 7.9 | 3.8 | 2.1 | pH 8, 37 °C | PMID 41754831 | Open record: tyrosine phenol-lyase (TPL), S-ethyl-L-cysteine sulfoxide |
| tyrosine phenol-lyase (TPL) | Citrobacter freundii | S-methyl-L-cysteine sulfoxide | 3.9 | 3.8 | 1 | pH 8, 37 °C | PMID 41754831 | Open record: tyrosine phenol-lyase (TPL), S-methyl-L-cysteine sulfoxide |
| tyrosine phenol-lyase (TPL) | Citrobacter freundii | S-propyl-L-cysteine sulfoxide | 15.8 | 3.7 | 4.3 | pH 8, 37 °C | PMID 41754831 | Open record: tyrosine phenol-lyase (TPL), S-propyl-L-cysteine sulfoxide |
| tyrosine phenol-lyase (TPL)variant F448A | Citrobacter freundii | L-tyrosine | 2.6 × 10⁻⁴ | — | — | 25 °C | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F448A, L-tyrosine |
| tyrosine phenol-lyase (TPL)variant F448A | Citrobacter freundii | S-(o-nitrophenyl)-L-cysteine | 2.5 | — | 9.6 | pH 8, 25 °C | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F448A, S-(o-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyase (TPL)variant F448A | Citrobacter freundii | S-ethyl-L-cysteine | 0.51 | — | 0.965 | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F448A, S-ethyl-L-cysteine | |
| tyrosine phenol-lyase (TPL)variant F448L | Citrobacter freundii | L-tyrosine | 0.005 | — | 0.0348 | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F448L, L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant F448L | Citrobacter freundii | S-(o-nitrophenyl)-L-cysteine | 0.26 | — | 4.5 | pH 8, 25 °C | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F448L, S-(o-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyase (TPL)variant F448L | Citrobacter freundii | S-ethyl-L-cysteine | 0.23 | — | 0.994 | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F448L, S-ethyl-L-cysteine | |
| tyrosine phenol-lyase (TPL)variant F449A | Citrobacter freundii | L-tyrosine | 1.6 × 10⁻⁴ | — | — | 25 °C | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F449A, L-tyrosine |
| tyrosine phenol-lyase (TPL)variant F449A | Citrobacter freundii | S-(o-nitrophenyl)-L-cysteine | 0.36 | — | 6.2 | pH 8, 25 °C | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F449A, S-(o-nitrophenyl)-L-cysteine |
| tyrosine phenol-lyase (TPL)variant F449A | Citrobacter freundii | S-ethyl-L-cysteine | 0.12 | — | 0.088 | doi:10.1021/acscatal.6b01495 | Open record: tyrosine phenol-lyase (TPL) variant F449A, S-ethyl-L-cysteine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | 3-(methylthio)-L-tyrosine | — | — | 0.0442 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, 3-(methylthio)-L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | 3-bromo-L-tyrosine | 0.25 | — | 0.06 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, 3-bromo-L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | 3-chloro-L-tyrosine | 0.38 | — | 0.43 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, 3-chloro-L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | 3-fluoro-L-tyrosine | 0.73 | — | 0.774 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, 3-fluoro-L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | 3-methoxy-L-tyrosine | 0.81 | — | 0.091 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, 3-methoxy-L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | 3-methyl-L-tyrosine | 0.1 | — | 0.0097 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, 3-methyl-L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | L-3,4-dihydroxyphenylalanine | — | — | 6.7 × 10⁻⁴ | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, L-3,4-dihydroxyphenylalanine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | L-tyrosine | — | — | 0.148 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, L-tyrosine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | S-(2-nitrophenyl)-L-cysteine | 7.1 | — | 96 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, S-(2-nitrophenyl)-L-cysteine | |
| tyrosine phenol-lyase (TPL)variant M379A | Citrobacter freundii | S-ethyl-L-cysteine | — | — | 0.21 | PMID 35577764 | Open record: tyrosine phenol-lyase (TPL) variant M379A, S-ethyl-L-cysteine |