CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structural basis for the alteration of coenzyme specificity in a malate dehydrogenase mutant

PMID 16828705doi:10.1016/j.bbrc.2006.06.131Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (66 values) from this paper: malate dehydrogenase (tMDH) from Thermus flavus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.37

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
malate dehydrogenase (tMDH)Thermus flavusNADH122.40.00246.11 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)Thermus flavusNADPH74.20.04261.7 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)Thermus flavusoxaloacetateNADH; cosubstrate NADH146.70.0034.89 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)Thermus flavusoxaloacetateNADPH; cosubstrate NADPH72.70.7402100pH 7, 30 °COpen
malate dehydrogenase (tMDH)Thermus flavusreduced nicotinamide hypoxanthine dinucleotide297.50.03757.9 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX3 (E41G/I42S/A45S)Thermus flavusNADH98.10.09451 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX3 (E41G/I42S/A45S)Thermus flavusNADPH140.50.09851.4 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX3 (E41G/I42S/A45S)Thermus flavusoxaloacetateNADH; cosubstrate NADH104.10.03173.3 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX3 (E41G/I42S/A45S)Thermus flavusoxaloacetateNADPH; cosubstrate NADPH145.70.00542.7 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX4 (E41G/I42S/Q44R/A45S)Thermus flavusNADH188.40.16411.1 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX4 (E41G/I42S/Q44R/A45S)Thermus flavusNADPH246.70.00514.83 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX4 (E41G/I42S/Q44R/A45S)Thermus flavusoxaloacetateNADH; cosubstrate NADH111.10.0151.26 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX4 (E41G/I42S/Q44R/A45S)Thermus flavusoxaloacetateNADPH; cosubstrate NADPH245.40.01172.1 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX6 (E41G/I42S/P43E/A45S/M46F/K47Q)Thermus flavusNADH173.40.16081.1 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX6 (E41G/I42S/P43E/A45S/M46F/K47Q)Thermus flavusNADPH192.20.02757 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX6 (E41G/I42S/P43E/A45S/M46F/K47Q)Thermus flavusoxaloacetateNADH; cosubstrate NADH86.80.0091.92 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX6 (E41G/I42S/P43E/A45S/M46F/K47Q)Thermus flavusoxaloacetateNADPH; cosubstrate NADPH184.70.03534.8 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX7 (E41G/I42S/P43E/Q44R/A45S/M46F/K47Q)Thermus flavusNADH80.20.0809900pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX7 (E41G/I42S/P43E/Q44R/A45S/M46F/K47Q)Thermus flavusNADPH111.70.00741.51 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX7 (E41G/I42S/P43E/Q44R/A45S/M46F/K47Q)Thermus flavusoxaloacetateNADH; cosubstrate NADH78.20.03172.5 × 10³pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX7 (E41G/I42S/P43E/Q44R/A45S/M46F/K47Q)Thermus flavusoxaloacetateNADPH; cosubstrate NADPH1120.00542.07 × 10⁴pH 7, 30 °COpen
malate dehydrogenase (tMDH)variant EX7 (E41G/I42S/P43E/Q44R/A45S/M46F/K47Q)Thermus flavusreduced nicotinamide hypoxanthine dinucleotide phosphate70.40.00729.7 × 10³pH 7, 30 °COpen