Values CatLog read from this paper
Kinetic and chemical mechanism of alpha-isopropylmalate synthase from Mycobacterium tuberculosis
PMID 16846242doi:10.1021/bi0606602Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (31 values) from this paper: α-isopropylmalate synthase from Mycobacterium tuberculosis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| α-isopropylmalate synthase | Mycobacterium tuberculosis | acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1 | 2.1 | 0.136 | 15 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, acetyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1 | 5 | 0.279 | 18 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, acetyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1 | 7.6 | 0.057 | 130 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, acetyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | acetyl-Coenzyme A20 mM KCl and 20 mM MgCl2 (Methods standard assay); Table 1 | 4.5 | 0.074 | 61 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, acetyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | acetyl-Coenzyme Awithout added MgCl2; Table 2 | 0.06 | 0.37 | 0.15 | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, acetyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | acetyl-Coenzyme Awith added MgCl2; Table 2 | 0.03 | 0.16 | 0.17 | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, acetyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | crotonyl-Coenzyme Awithout added MgCl2 | 0.05 | 0.05 | 0.94 | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, crotonyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | crotonyl-Coenzyme Awith added MgCl2 | 0.04 | 0.095 | 0.4 | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, crotonyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | propionyl-Coenzyme A | 0.04 | 0.22 | 0.19 | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, propionyl-Coenzyme A |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | pyruvate | 6.1 | 9.5 | 0.64 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, pyruvate |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | α-ketobutyrate | 10.7 | 0.86 | 12 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, α-ketobutyrate |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | α-ketoisovalerate | 3.5 | 0.012 | 290 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, α-ketoisovalerate |
| α-isopropylmalate synthase | Mycobacterium tuberculosis | α-ketovalerate | 7 | 0.41 | 17 calc. | pH 7.4, 25 °C | Open record: α-isopropylmalate synthase, α-ketovalerate |